Trex1 (NM_001024989) Rat Tagged ORF Clone

CAT#: RR203882

Trex1 (Myc-DDK-tagged ORF) - Rat three prime repair exonuclease 1 (Trex1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trex1
• Synonyms: RGD1309596
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203882 representing NM_001024989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCACAGGCCCTGCCCCACGGTCACATGCAGACCCTCATCTTCTTAGACCTGGAAGCCACTGGCC
  TGCCTTACTCTCAGCCCAAAATCACAGAGCTGTGCCTGCTGGCTGTCCACAGACATGCCCTGGAGAACTC
  CTCCATGTCTGAGGGGCAGCCACCTCCGGTGCCCAAACCACCCCGTGTGGTGGACAAGCTCTCTCTCTGC
  ATTGCTCCAGGGAAACCCTGCAGTTCTGGGGCCAGTGAGATCACAGGTCTGACCACAGCTGGGCTGGAAG
  CACACGGGCGTCAACGCTTCAATGACAACCTGGCCACCCTGCTCCAAGTCTTCCTGCAGCGCCAGCCACA
  GCCCTGCTGCCTTGTGGCCCACAACGGTGACCGCTACGACTTTCCTCTGCTCCAGGCAGAGCTTGCTTCG
  CTGAGCGTTATCAGTCCTCTGGACGGTACCTTCTGTGTGGATAGCATCGCTGCCCTGAAGACCTTGGAGC
  AAGCCAGCAGCCCCTCAGAGCATGGTCCGAGGAAAAGCTATAGCCTGGGTAGTATCTACACCCGCCTGTA
  CGGGCAAGCACCAACAGACTCACACACTGCTGAAGGCGACGTTCTAGCCCTGCTCAGCATCTGTCAGTGG
  AAGCCACAGGCCCTACTGCAGTGGGTGGACAAACACGCCCGGCCCTTTAGCACCATCAAGCCCATGTATG
  GCATGGCAGCTACTACTGGAACGGCCAGCCCAAGGCTGTGTGCTGCCACAACCTCCTCACCCCTGGCCAC
  AGCCAACTTAAGTCCCAGCAATGGCAGGAGCAGGGGCAAGCGACCTACGAGTCCTCCTCCAGAGAACGTC
  CCAGAAGCCCCATCACGGGAGGGGCTGCTGGCCCCACTGGGCCTGCTGACCTTCCTGACCTTGGCAATAG
  CCGTTCTGTATGGAATCTTCCTGGCCTCACCTGGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203882 representing NM_001024989
  Red=Cloning site Green=Tags(s)
  
  MGSQALPHGHMQTLIFLDLEATGLPYSQPKITELCLLAVHRHALENSSMSEGQPPPVPKPPRVVDKLSLC
  IAPGKPCSSGASEITGLTTAGLEAHGRQRFNDNLATLLQVFLQRQPQPCCLVAHNGDRYDFPLLQAELAS
  LSVISPLDGTFCVDSIAALKTLEQASSPSEHGPRKSYSLGSIYTRLYGQAPTDSHTAEGDVLALLSICQW
  KPQALLQWVDKHARPFSTIKPMYGMAATTGTASPRLCAATTSSPLATANLSPSNGRSRGKRPTSPPPENV
  PEAPSREGLLAPLGLLTFLTLAIAVLYGIFLASPGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024989
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024989.1, NP_001020160.1
• RefSeq Size: 1101 bp
• RefSeq ORF: 951 bp
• Locus ID: 100049583
• Cytogenetics: 8q32
• MW: 33.6 kDa