Itgam (NM_012711) Rat Tagged ORF Clone

CAT#: RR203904

Itgam (Myc-DDK-tagged ORF) - Rat integrin alpha M (Itgam), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgam
• Synonyms: Cd11b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203904 representing NM_012711
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTTAAAGTTCTTCTGGCGACAGTCCTAACCTTGTGTCATGGCTTCAATTTGGACACTGAAAATC
  CCATGACCTTCCAAGAGAATGCAAGTGGCTTCGGGCAGAGTGTGATCCAGCTTGGTGAAACCCGAGTGGT
  TGTTGCAGCCCCCCAGGAGGTAAAGGCGGTTAACCAGACAGGTGCCCTCTACCAGTGTGACTACAGCACA
  AACCGGTGCGACCCCATTCCCCTGCAAGTACCTCCAGAGGCTGTGAATATGTCCTTGGGCCTGTCCCTGG
  CTGCTACTACCGTCCCTCCACAGCTGCTGGCTTGTGGCCCCACAGTACACCAGAACTGCAAGGAGAACAC
  TTACGTCAATGGATTGTGCTACCTATTCGGCTCCAACCTGCTGAGGAAGCCCCAGCAGTTCCCAGAGGCT
  CTCAGAGGATGTCCTCAGCAGGAGAGCAACATTGCCTTCTTGATTGACGGCTCCGGTAGCATCAACACGA
  TCGACTTTCAGAAGATGAAGGAATTTGTCTCAACTGTGATGGACCAGTTCCAAAAGTCTAAAACCTTGTT
  CTCTTTGATGCAGTACTCTGATGAATTCCGGACTCACTTCACCTTCAATGATTTCAAGAGAAACCCTGAC
  CCAAAATCACATGTGAGACCCATAAGGCAGCTGAATGGAAGGACAAAAACTGCCTCAGGGATCCGTAAAG
  TAGTGAGAGAACTGTTTCAGAAAATCAATGGGGCCCGGGACAATGCCGCGAAGATCCTAGTTGTCATCAC
  AGACGGAGAAAAGTTTGGTGACCCCCTAAATTATGAGGATGTCATTCCTGAGGCAGAGGAAGCAGGGATC
  ATTCGCTACGTTATTGGGGTGGGAAACGCCTTCCACAAACCACAGTCCCGCAGAGAGCTTGACACCATCG
  CGTCTAAGCCAGCTGGTGATCACGTGTTCCAAGTGGACAACTTTGAAGCTCTGAATACCATTCGGAACCA
  GCTCCAGGAAAAGATCTTTGCAATTGAGGGCACCCAGACAGGAAGTACCAGCTCCTTTGAGCATGAGATG
  TCTCAAGAAGGCTTTAGCGCTGCCATTACCTCCAACGGTCCCTTGCTGGGCTCCGTGGGGAGCTTTGACT
  GGGCAGGTGGAGCCTTCCTGTATCCATCAAAGGATAAAGCCAGTTTCATCAACACAACCAGAATAGATTC
  AGACATGAACGATGCTTACTTGGGTTATGCTTCTGCAGTCATCTCGAGGAACCGCGTCCAAAGCTTGGTT
  TTAGGGGCACCTCGATATCAGCATATCGGCCTGGTGGTGATGTTCAAGCAGAATTTCGGTGCCTGGGAGC
  CCCACACTGATATCAAGGGCAGCCAGATCGGCTCTTATTTTGGGGCCTCCCTCTGTTCTGTGGACATGGA
  CGCTGATGGCAATACCAACTTGATCCTCATTGGGGCCCCTCATCACTATGAGAAGACCCGAGGGGGCCAG
  GTGTCAGTGTGCCCCTTGCCTAGGGGGAGAGCACGGTGGCAGTGTGAAGCTATTCTCCATGGCGATCAGG
  GCCATCCATGGGGTCGCTTTGGGGCAGCATTGACAGTGCTGGGAGATGTGAATGGAGACAAACTGACAGA
  TGTGGCCATTGGGGCCCCAGGAGAGCAGGAGAATCAGGGTGCTGTCTACATTTTTCATGGAGCATCAGTA
  GCCAGCATCAGTACCCCTCACAGCCAGCGGATCGCAGGCGCCCGCTTCTCCCCCGGGCTCCAGTACTTTG
  GGCAGTCTCTGAGTGGGGGCAAGGATCTCACAATGGACGGCCTGATGGACTTGGCTGTGGGGGCGCAGGG
  GCGTCTTCTGCTGATCAGAGCCCAGCCTGTGCTGAGACTGGAGGCAACCATGGAATTCAGCCCCAAGAAT
  CTAGCAAGGAGTGTGTTTGCGTGTCAAGAAGAAGTAGTCAAAAACAAGGATGCTGGGGAGGTCAGAGTCT
  GCCTCCAAGTCCGCAAGAACACCAAGGACAGGCTGCGAGAAGGAGACATCCAGAGCACTGTCACTTATGA
  CCTGGCTTTAGACCCCGGCCGCTCAGTTGTCCGAGCCTTCTTTGATGAAACAAAGAACGGCATACTCAGG
  CGCATCCGGGTCTTTGGATTGACACAGAAATGTGAAACCCTGAAGCTAATTTTACCGGACTGTGTGGACA
  ACTCAGTGAGTCCCATCATCCTGCGCCTTAATTACACACTGGTTGGGGAGCCCTTGCGGTCCTCTAGGGA
  CCTTCGGCCAGTTCTGGCTATGGAGGCTCAGAGGATCTTCACAGCTATGTTTCCCTTTGAGAAGAATTGT
  GGCAATGACACCATTTGCCAGGATGATCTCAGCATCACCGTGAGTTCCACACGCGTGAACACTTTGGTGG
  TGGGAGACTCCCGGGACTTTGACGTGAGTGTGACTCTGAGAAATGATGGCGAGGATTCCTATGGGACCAA
  GGTTACCTGCTATTACCCATCTGGCTTGTCTTACCGGAAGGTGTCAGCAAGCCAGAACCAGTTCTCCAAG
  AAGCCTTGGCGTGTGATAGCTGAGCCCAGTTCTTCTGAAGGGCAAGGGGTTCTGAAGAGCACCATCTGGG
  ACATAAACCATCCCATCTTTCCCGCTAATTCTGAGGTCACGTTTAATGTCACATTTGATGTGGACTCTGA
  TGCCTCCCTTGGGAACAAACTGCTCCTCAAGGTCGTTGTGACCAGTGAGAACAACGTGTCCAGGACCGAC
  AAAACTGAGTCTCAGTTGGAGTTGCCTGTGAAGTACGCCATCTACATGGTCGTCACCAGTGGTGAGAGTT
  CTATCAAATATCTCAACTTCACAGCTTCAGAGATGACCAGTAAGGTCATACAGCATCAGTACCAGTTCAA
  CAACCTGGGCCAGAGGAGCTTCCCTGTCAGCGTGGTCTTCTGGATCCCTGTCCAGATCAACAAGGTGACC
  ATATGGGATCCTCCCCAGGTCACCTTCTCCCAGAACCTCTCAAGTGTCTGTCGCACTGAGCAGAAATCCC
  CCTCTCACTCCAAGTTCCAGGATGAGCTTGAAAGGACCCCAGTGCTGAACTGCTCTGTTGCAGTCTGCAA
  GAGAATCCAGTGTGACATCCCTTCCTTCAACAGTAAAGAAATATTCAACGTCACCCTCCAGGGCAATCTG
  CTATTTGACTGGTACATCGAGACTTCTCATGACCACCTCCTGCTTGTGAGCACTGCCGAGATCCTCTTTA
  ACGACTCCGCATTTGCCCTACTTCCGGGGCAGGAGACGTTTGTGAAGGCTCAGACAGAGACCAAAGTGGA
  GCCATATACAGTTCACAATCCTGTACCACTCATTGTGGGCAGCTCCGTCGGAGGCCTGGTGCTCTTGGCT
  CTCATCACTGCTGGCCTATACAAGCTTGGCTTTTTCAAACGACAGTACAAGGACATGATGAATGAAGCCG
  GTGGCCAAGATGGCCCACCTCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203904 representing NM_012711
  Red=Cloning site Green=Tags(s)
  
  MTLKVLLATVLTLCHGFNLDTENPMTFQENASGFGQSVIQLGETRVVVAAPQEVKAVNQTGALYQCDYST
  NRCDPIPLQVPPEAVNMSLGLSLAATTVPPQLLACGPTVHQNCKENTYVNGLCYLFGSNLLRKPQQFPEA
  LRGCPQQESNIAFLIDGSGSINTIDFQKMKEFVSTVMDQFQKSKTLFSLMQYSDEFRTHFTFNDFKRNPD
  PKSHVRPIRQLNGRTKTASGIRKVVRELFQKINGARDNAAKILVVITDGEKFGDPLNYEDVIPEAEEAGI
  IRYVIGVGNAFHKPQSRRELDTIASKPAGDHVFQVDNFEALNTIRNQLQEKIFAIEGTQTGSTSSFEHEM
  SQEGFSAAITSNGPLLGSVGSFDWAGGAFLYPSKDKASFINTTRIDSDMNDAYLGYASAVISRNRVQSLV
  LGAPRYQHIGLVVMFKQNFGAWEPHTDIKGSQIGSYFGASLCSVDMDADGNTNLILIGAPHHYEKTRGGQ
  VSVCPLPRGRARWQCEAILHGDQGHPWGRFGAALTVLGDVNGDKLTDVAIGAPGEQENQGAVYIFHGASV
  ASISTPHSQRIAGARFSPGLQYFGQSLSGGKDLTMDGLMDLAVGAQGRLLLIRAQPVLRLEATMEFSPKN
  LARSVFACQEEVVKNKDAGEVRVCLQVRKNTKDRLREGDIQSTVTYDLALDPGRSVVRAFFDETKNGILR
  RIRVFGLTQKCETLKLILPDCVDNSVSPIILRLNYTLVGEPLRSSRDLRPVLAMEAQRIFTAMFPFEKNC
  GNDTICQDDLSITVSSTRVNTLVVGDSRDFDVSVTLRNDGEDSYGTKVTCYYPSGLSYRKVSASQNQFSK
  KPWRVIAEPSSSEGQGVLKSTIWDINHPIFPANSEVTFNVTFDVDSDASLGNKLLLKVVVTSENNVSRTD
  KTESQLELPVKYAIYMVVTSGESSIKYLNFTASEMTSKVIQHQYQFNNLGQRSFPVSVVFWIPVQINKVT
  IWDPPQVTFSQNLSSVCRTEQKSPSHSKFQDELERTPVLNCSVAVCKRIQCDIPSFNSKEIFNVTLQGNL
  LFDWYIETSHDHLLLVSTAEILFNDSAFALLPGQETFVKAQTETKVEPYTVHNPVPLIVGSSVGGLVLLA
  LITAGLYKLGFFKRQYKDMMNEAGGQDGPPQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012711
• ORF Size: 3453 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012711.1, NP_036843.1
• RefSeq Size: 3456
• RefSeq ORF: 3456
• Locus ID: 25021
• MW: 126.9 kDa
• Gene Summary: mouse homolog is an integrin alpha subunit that interacts with integrin beta 2 subunit to form Mac-1; involved in immune response [RGD, Feb 2006]