Alox15 (NM_031010) Rat Tagged ORF Clone

CAT#: RR204005

Alox15 (Myc-DDK-tagged ORF) - Rat arachidonate 15-lipoxygenase (Alox15), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alox15
• Synonyms: 12-LOX; 15-LOX; Alox12; Alox12l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204005 representing NM_031010
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTCTACCGCATCCGCGTCTCCACCGGAGACTCCAAGTACGCGGGCTCCAACAACGAGGTCTACC
  TGTGGTTGGTTGGACAGCATGGAGAGGCATCTCTCGGGAAGCTGCTACGACCCTGTCGGGACTCGGAAGC
  AGAATTCAAAGTGGATGTGTCAGGATACCTTGGGCCACTGCTGTTCGTAAGAGTGCAGAAATGGCATTAT
  CTCACGGATGACGCCTGGTTCTGCAACTGGATTTCTGTGAAGGGCCCCGGAGACCAAGGATCAGAGTACA
  TGTTCCCCTGTTACCGATGGGTTCAGGGCAGAAGCATCCTGAGCCTCCCTGAGGGCACTGGCTGCACCGT
  GGTTGAAGATTCTCAAGGACTGTTCAGGAAACATAGGGAAGAGGAGCTTGAAGAGAGGAGGAGTCTGTAC
  AGGTGGGGCAACTGGAAGGATGGCTCAATCCTGAATGTGGCGGCGGCCAGTATATCTGACCTCCCTGTAG
  ACCAACGATTTCGAGAGGACAAAAGAATTGAATTTGAAGCTTCACAGGTTATAGGGGTAATGGATACTGT
  TGTCAACTTTCCTATAAACACTGTGACCTGCTGGAAAAGCCTAGATGACTTCAACTGCGTTTTCAAGAGT
  GGCCATACCAAAATGGCTGAGCGGGTTCGAAACTCCTGGAAGGAAGATGCGTTCTTTGGGTACCAATTCC
  TCAATGGTGCTAACCCCATGGTGCTGAAGCGCTCTACTTGTCTTCCTGCCCGCCTGGTATTCCCTCCAGG
  AATGGAGAAGCTACAGGCCCAGCTGAACAAGGAGCTCCAGAAAGGCACTCTGTTTGAAGCGGATTTCTTC
  CTTCTGGATGGGATCAAGGCCAATGTCATCCTTTGTAGCCAGCAGTACCTGGCTGCCCCTCTCGTCATGC
  TGAAGCTGATGCCTGATGGACAACTCTTGCCCATAGCCATCCAGCTTGAACTTCCCAAAACTGGGTCTAC
  TCCACCACCTATTTTCACGCCCTCGGATCCCCCAATGGACTGGCTCCTAGCCAAATGCTGGGTCCGGAGC
  TCCGACTTACAGCTCCATGAGCTGCAGGCTCATCTTCTGAGGGGACACTTGATGGCTGAGGTCTTTGCTG
  TGGCCACCATGAGGTGCCTGCCTTCCGTGCACCCTGTTTTTAAGCTTCTAGTTCCTCATCTGCTTTACAC
  CATGGAAATTAATGTCCGGGCCAGGAGTGACCTGATCTCAGAGAGAGGCTTTTTTGACAAGGCAATGAGC
  ACAGGTGGGGGAGGCCACCTGGATCTTCTCAAGCAAGCTGGAGCCTTTCTGACCTATTGCTCATTGTGTC
  CCCCCGATGACTTGGCTGAGCGAGGACTCTTGGATATCGAGACTTGCTTCTATGCTAAAGACGCCCTGCG
  ACTCTGGCAGATCATGAATCGGTACGTGGTGGGAATGTTCAATCTCCACTACAAGACCGACAAAGCTGTG
  CAAGACGACTATGAACTGCAGAGCTGGTGTCGAGAGATCACTGACATTGGTCTTCAAGGGGCCCAGGACA
  GAGGCTTCCCTACCTCTCTTCAGTCCCGGGCTCAGGCTTGCTACTTCATCACCATGTGCATCTTCACGTG
  CACCGCACAGCACTCTTCCGTCCATCTTGGCCAGCTGGATTGGTTCTACTGGGTTCCTAATGCACCCTGC
  ACCATGCGGCTGCCACCACCCACCACCAAGGAAGCAACAATGGAGAAGCTGATGGCTACACTGCCCAACC
  CTAATCAGTCTACTCTCCAGATAAATGTCGTTTGGCTCCTGGGCAGACGCCAGGCTGTTATGGTGCCCCT
  GGGCCAGCATTCAGAGGAACACTTTCCAAACCCTGAGGCCAAGGCTGTGCTGAAGAAGTTCAGAGAGGAA
  CTGGCTGCCTTGGATAAGGAAATTGAGATTCGTAATAAGAGCTTGGACATACCTTATGAGTACCTGCGGC
  CCAGCATGGTGGAAAACAGCGTGGCCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204005 representing NM_031010
  Red=Cloning site Green=Tags(s)
  
  MGVYRIRVSTGDSKYAGSNNEVYLWLVGQHGEASLGKLLRPCRDSEAEFKVDVSGYLGPLLFVRVQKWHY
  LTDDAWFCNWISVKGPGDQGSEYMFPCYRWVQGRSILSLPEGTGCTVVEDSQGLFRKHREEELEERRSLY
  RWGNWKDGSILNVAAASISDLPVDQRFREDKRIEFEASQVIGVMDTVVNFPINTVTCWKSLDDFNCVFKS
  GHTKMAERVRNSWKEDAFFGYQFLNGANPMVLKRSTCLPARLVFPPGMEKLQAQLNKELQKGTLFEADFF
  LLDGIKANVILCSQQYLAAPLVMLKLMPDGQLLPIAIQLELPKTGSTPPPIFTPSDPPMDWLLAKCWVRS
  SDLQLHELQAHLLRGHLMAEVFAVATMRCLPSVHPVFKLLVPHLLYTMEINVRARSDLISERGFFDKAMS
  TGGGGHLDLLKQAGAFLTYCSLCPPDDLAERGLLDIETCFYAKDALRLWQIMNRYVVGMFNLHYKTDKAV
  QDDYELQSWCREITDIGLQGAQDRGFPTSLQSRAQACYFITMCIFTCTAQHSSVHLGQLDWFYWVPNAPC
  TMRLPPPTTKEATMEKLMATLPNPNQSTLQINVVWLLGRRQAVMVPLGQHSEEHFPNPEAKAVLKKFREE
  LAALDKEIEIRNKSLDIPYEYLRPSMVENSVAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031010
• ORF Size: 1989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031010.1, NM_031010.2, NP_112272.2
• RefSeq Size: 2216
• RefSeq ORF: 1992
• Locus ID: 81639
• MW: 75.3 kDa
• Gene Summary: demonstrates 12-lipoxygenase and some 15-lipoxygenase enzyme activity [RGD, Feb 2006]