Lmna (NM_001002016) Rat Tagged ORF Clone

CAT#: RR204013

Lmna (Myc-DDK-tagged ORF) - Rat lamin A (Lmna), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lmna
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204013 representing NM_001002016
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCCGTCTCAGCGGCGCGCCACCCGCAGTGGGGCGCAGGCCAGCTCTACCCCGCTGTCGCCCA
  CTCGGATCACACGGCTGCAGGAGAAGGAGGACCTGCAGGAGCTCAATGACCGTCTGGCCGTGTACATCGA
  TCGCGTGCGCTCCCTGGAGACGGAGAACGCGGGGCTGCGCCTTCGCATCACTGAGTCTGAAGAGGTGGTC
  AGCCGAGAGGTGTCCGGCATCAAGGCGGCCTACGAGGCCGAGCTGGGGGATGCCCGCAAGACCCTCGACT
  CTGTGGCCAAGGAGCGCGCCCGCCTCCAGTTGGAGCTGAGCAAAGTGCGTGAGGAGTTCAAGGAGCTGAA
  GGCGCGCAATACCAAGAAGGAGGGGGATTTGTTGGCTGCGCAGGCCCGGCTCAAGGACCTGGAGGCTCTT
  CTCAACTCCAAGGAGGCTGCCCTGAGCACTGCTCTCAGTGAGAAACGCACGTTGGAAGGCGAGCTCCATG
  ATCTGCGGGGGCAGGTAGCCAAGCTTGAGGCAGCCCTGGGTGAGGCTAAGAAGCAACTCCAGGATGAGAT
  GCTGAGGCGAGTGGATGCTGAGAACAGGCTACAGACCCTGAAGGAGGAGCTGGATTTCCAGAAGAACATT
  TACAGTGAGGAACTGCGTGAGACCAAGCGCCGGCACGAGACCCGGCTGGTGGAGATTGATAATGGGAAGC
  AGCGAGAGTTTGAGAGCCGGCTGGCAGATGCTCTGCAGGAGCTGCGGGCCCAGCATGAGGACCAGGTGGA
  GCAGTACAAGAAGGAGCTAGAAAAGACATACTCTGCCAAGCTGGATAATGCCCGGCAGTCTGCCGAGAGG
  AACAGCAACCTCGTGGGAGCTGCCCATGAGGAGCTCCAGCAGTCTCGAATCCGCATTGACAGCCTCTCAG
  CCCAGCTCAGCCAGCTCCAGAAGCAGTTGGCAGCCAAGGAGGCCAAGCTGCGTGACCTGGAGGACTCCCT
  GGCCCGTGAGCGGGATACCAGCCGGCGCCTGCTGGCTGAAAAAGAGCGGGAGATGGCTGAGATGCGGGCA
  AGGATGCAGCAGCAGCTGGATGAGTACCAGGAATTGCTGGACATCAAGCTGGCCCTGGACATGGAGATCC
  ATGCCTACCGAAAGCTGCTGGAGGGCGAGGAGGAGAGGCTACGCCTGTCCCCCAGCCCTACCTCGCAGCG
  CAGCCGTGGCCGCGCCTCCTCCCACTCCTCCCAGTCTCAGGGTGGAGGCAGCGTCACCAAAAAGCGCAAG
  CTGGAGTCTTCTGAGAGCCGGAGCAGCTTCTCGCAGCATGCTCGCACTAGCGGCCGTGTGGCCGTAGAGG
  AAGTAGATGAAGAGGGCAAGTTCGTGCGGCTGCGCAACAAGTCCAACGAGGACCAGTCCATGGGCAACTG
  GCAGATCAGGCGTCAGAATGGTGATGATCCTTTGATGACTTATCGCTTCCCACCGAAGTTCACCCTAAAG
  GCCGGGCAGGTGGTGACGATCTGGGCTTCAGGAGCTGGGGCCACCCATAGCCCCCCTACTGACTTGGTGT
  GGAAGGCACAGAACACTTGGGGCTGTGGGAGCAGCCTTCGCACGGCTCTCATCAACGCCACTGGAGAAGA
  AGTGGCCATGCGCAAGCTGGTGCGCTCACTGACCATGGTTGAGGACAATGACGATGAGGAAGAGGATGGA
  GATGAGCTGCTCCATCACCATCGTGGCTCCCACTGCAGCAGCTCGGGGGACCCCGCTGAGTACAACCTGC
  GCTCACGCACCGTGCTGTGCGGGACATGTGGGCAGCCTGCTGACAAGGCTGCCAGTGGCTCAGGAGCCCA
  GGTGGGCGGATCCATCTCCTCTGGCTCTTCTGCCTCCAGTGTCACAGTCACTCGAAGCTTCCGCAGTGTG
  GGGGGCAGTGGGGGTGGCAGCTTCGGGGACAACCTAGTCACCCGCTCCTACCTCCTGGGCAACTCCAGTC
  CCCGGACCCAGAGCTCCCAGAACTGCAGCATCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204013 representing NM_001002016
  Red=Cloning site Green=Tags(s)
  
  METPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVV
  SREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLLAAQARLKDLEAL
  LNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTLKEELDFQKNI
  YSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAER
  NSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEKEREMAEMRA
  RMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQSQGGGSVTKKRK
  LESSESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIRRQNGDDPLMTYRFPPKFTLK
  AGQVVTIWASGAGATHSPPTDLVWKAQNTWGCGSSLRTALINATGEEVAMRKLVRSLTMVEDNDDEEEDG
  DELLHHHRGSHCSSSGDPAEYNLRSRTVLCGTCGQPADKAASGSGAQVGGSISSGSSASSVTVTRSFRSV
  GGSGGGSFGDNLVTRSYLLGNSSPRTQSSQNCSIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002016
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002016.2, NP_001002016.2
• RefSeq Size: 3112 bp
• RefSeq ORF: 1998 bp
• Locus ID: 60374
• Cytogenetics: 2q34
• MW: 74.3 kDa
• Gene Summary: a component of the nuclear membrane on the nucleoplasmic side or lamina [RGD, Feb 2006]