Smpd2 (NM_031360) Rat Tagged ORF Clone

CAT#: RR204041

Smpd2 (Myc-DDK-tagged ORF) - Rat sphingomyelin phosphodiesterase 2, neutral (Smpd2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smpd2
• Synonyms: nSMase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204041 representing NM_031360
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCACAACTTTTCTCTGCGGCTGAGGGTTTTCAACCTCAACTGCTGGGACATCCCCTACCTAAGCA
  AGCATAGGGCCGACCGCATGAAGCGCTTGGGAGACTTTCTGAACTTGGAGAGCTTTGACCTGGCTCTGCT
  GGAGGAGGTGTGGAGTGAGCAGGACTTCCAGTACTTAAAGCAAAAGCTATCGCTCACCTACCCGGATGCA
  CACTACTTCAGAAGCGGAATCATTGGCAGTGGCCTCTGTGTGTTCTCCAGACACCCAATCCAGGAAATTG
  TCCAGCACGTCTATACTCTCAATGGTTACCCCTACAAGTTCTATCATGGAGACTGGTTCTGTGGGAAGGC
  TGTGGGGCTGCTGGTGCTCCATCTAAGCGGACTGGTGCTCAACGCCTACGTGACCCATCTGCACGCTGAG
  TACAGCCGGCAGAAGGATATCTACTTCGCACACCGTGTGGCCCAAGCTTGGGAACTGGCCCAGTTCATCC
  ACCACACATCCAAGAAAGCAAATGTGGTCCTGTTGTGCGGGGATCTCAACATGCACCCCAAAGACCTGGG
  CTGCTGCCTACTGAAAGAGTGGACGGGGCTCCGTGATGCCTTTGTTGAGACCGAGGACTTTAAGGGCTCA
  GAGGACGGCTGTACCATGGTACCCAAGAACTGCTACGTCAGCCAGCAGGACCTGGGGCCGTTTCCCTTTG
  GCGTCCGTATCGACTACGTGCTTTACAAGGCAGTCTCTGGCTTCCACATCTGCTGTAAGACTCTGAAAAC
  CACTACGGGCTGTGACCCCCACAATGGCACCCCCTTCTCTGACCACGAGGCCCTCATGGCTACTTTGTGT
  GTGAAGCACAGCCCCCCTCAGGAAGACCCTTGCTCTGCCCATGGATCAGCGGAAAGGTCAGCATTGATCA
  GCGCGCTAAGGGAGGCGAGGACAGAGCTGGGGCGAGGCATAGCTCAAGCTCGCTGGTGGGCTGCACTCTT
  TGGCTATGTGATGATTTTGGGGCTGTCCCTCCTGGTGTTGCTGTGTGTCCTGGCTGCAGGAGAAGAGGCC
  AGGGAAGTGGCCATAATGCTCTGGACACCCAGTGTGGGTCTGGTGCTGGGGGCAGGCGCAGTCTACCTGT
  TCCACAAGCAGGAGGCCAAAAGCTTATGTCGGGCCCAGGCTGAGATACAACATGTTCTGACAAGGACAAC
  GGAGACCCAGGACCTAGGCTCAGAGCCTCACCCAACCCACTGCCGGCAGCAGGAGGCGGACAGAGCTGAA
  GAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204041 representing NM_031360
  Red=Cloning site Green=Tags(s)
  
  MKHNFSLRLRVFNLNCWDIPYLSKHRADRMKRLGDFLNLESFDLALLEEVWSEQDFQYLKQKLSLTYPDA
  HYFRSGIIGSGLCVFSRHPIQEIVQHVYTLNGYPYKFYHGDWFCGKAVGLLVLHLSGLVLNAYVTHLHAE
  YSRQKDIYFAHRVAQAWELAQFIHHTSKKANVVLLCGDLNMHPKDLGCCLLKEWTGLRDAFVETEDFKGS
  EDGCTMVPKNCYVSQQDLGPFPFGVRIDYVLYKAVSGFHICCKTLKTTTGCDPHNGTPFSDHEALMATLC
  VKHSPPQEDPCSAHGSAERSALISALREARTELGRGIAQARWWAALFGYVMILGLSLLVLLCVLAAGEEA
  REVAIMLWTPSVGLVLGAGAVYLFHKQEAKSLCRAQAEIQHVLTRTTETQDLGSEPHPTHCRQQEADRAE
  EK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031360
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031360.1, NM_031360.2, NP_112650.1
• RefSeq Size: 1676
• RefSeq ORF: 1269
• Locus ID: 83537
• MW: 47.6 kDa
• Gene Summary: catalyzes the Mg2+ dependent hydrolysis of sphingomyelin to form N-acylsphingosine and choline phosphate; also has a lower activity for the hydrolysis of lyso-platelet activating factor to 1-alkylglycerol [RGD, Feb 2006]