Arhgef6 (NM_001005565) Rat Tagged ORF Clone

CAT#: RR204115

Arhgef6 (Myc-DDK-tagged ORF) - Rat Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (Arhgef6), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204115 representing NM_001005565
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCAGAAGAACGCGTTGTGACCTGGCTTATATCTTTGGGAGTTCTAGAGTCCCCCAAAAAGACCA
  TCTGTGATCCAGAAGAGTTCTTGAAGTCTTCGCTGAAAAATGGGGTAGTGTTGTGCAAACTCATAAGCAG
  ACTTCTGCCTGGCTCGGTGGAGAAGTATTGTCAGGAACCCCAAACTGAGGCCGACTGCATCGACAACATC
  AATGACTTCCTGAAAGGATGTGCAACCCTTCAAGTGGAAGTGTTTGAGCCTGATGACCTCTATTCTGGGG
  CCAATTTCTCCAAGGTTCTGAATACCCTTCTAGCTGTCAACAAAGCGACAGAAGATCAGCTATCAGAAAG
  ACCATGTGGACGGTCCTCTTCTCTTAGTGCTACGACCAGCTCTCAGACAAACCCACAGGCAGCCGTTCCC
  AGCACAACTCCAGAGCAGCAAAGCGAGGAAAAGGCAGCGGAGATGACGGAAAATGGAAGTCACCAGTTGA
  TAGTAAAGGCAAGATTCAACTTCAAGCAGACCAATGAAGATGAATTGTCAGTGTGTAAAGGGGACATCAT
  TTATGTCACTCGAGTTGAAGAAGGAGGCTGGTGGGAAGGCACATTAAATGGGAGAACCGGATGGTTCCCT
  AGTAATTATGTTCGAGAAATTAAACCCAGTGAGAGACCTCTGTCCCCTAAGGCTGTCAAAGGATTTGACA
  CTGCTCCACTTACCAAGAATTACTACACTGTGGTGTTACAGAACATCTTGGACACTGAAAAAGAATATGC
  CAAAGAACTTCAGTCTCTTCTTGTTACTTACCTAAGACCTTTGCAGTCTAATAACAACCTGAGTACTGTG
  GAGTTTACATGCTTATTGGGAAACTTTGAAGAAGTATGCACATTTCAACAGACACTCTGCCAAGCCCTGG
  AAGAATGCTCAAAGTCTCCAGAAAACCAGCACAAAGTAGGAGGCTGCCTACTGAACCTCATGCCTCATTT
  TAAATCGATGTACCTGGCTTACTGTGCAAATCATCCTTCAGCTGTCAACGTGCTCACCCAGCATAGTGAT
  GATCTAGAACGATTCATGGAAAATCAAGGTGCATCCAGCCCCGGTATCCTCATTTTAACAACAAGTCTCA
  GCAAACCATTCATGCGACTGGAAAAGTATGTTACTCTCTTGCAAGAACTGGAACGGCATATGGAGGACAC
  TCATCCAGATCATCAGGATATTCTGAAAGCTATCATAGCATTCAAAAGCCTCATGGGGCAGTGCCAAGAT
  CTGAGAAAGAGAAAGCAGCTGGAATTGCAGATCCTTTCGGAACCTATTCAGGCATGGGAAGGAGATGATA
  TTAAGACTTTGGGAAATGTGATCTTTATGTCACAAGTAGTGATGCAGCATGGAGCCTGTGAGGAAAAAGA
  GGAACGATATTTTCTGCTCTTTTCAAGTGTCTTGATCATGTTGTCTGCAAGTCCGCGGATGAGTGGCTTT
  ATGTATCAGGGAAAGGTACCAATAGCAGGGATGGTCGTGACCAGATTGGATGAAATTGAAGGAAATGACT
  GTACATTCGAAATCACCGGTAGCACGGTAGAGAGAATTGTGGTCCACTGCAACAATAACCAGGACTTCCA
  GGAGTGGATGGAGCAGCTGAACAGACTGACCAAAGGACCAGCCTCCTGCAGTTCATTGTCTAAGACCTCC
  TCATCATCATGTAGTACTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTGGAGCCTCCTC
  AAATTATAAAACCGTGGAGTTTAAGTTGCCTACGACCTGCACCTCCACTTAGACCATCAGCAGCACTAGG
  TTATAAAGAGAGGATGTCTTATATCTTAAAGGAATCTAGTAAGAGCCCCAAAACGATGAAGAAATTTCTT
  CACAAAAGAAAGACGGAGAGAAAGACATCCGAGGAAGAATACGTGATTAGGAAAAGTACTGCTGCCCTGG
  AAGAGGATGCTCAGATTCTTAAGGTGATTGAAGCTTATTGCACCAGCGCCAGTTTTCAACAAGGTACTCG
  TAAAGATTCAGTTCCACAAGTACTACTCCCCGAGGAAGAGAAACTCATTATTGAAGAAACTAGAAGTAAT
  GGGCAGACCATCATTGAAGAGAAGAGCCTTGTTGATACTGTCTATGCCTTGAAGGATGAGGTCAAGGAAT
  TGAAGCAGGAGAATAAAAAAATGAAGCAATGTCTGGAAGAAGAATTGAAGTCGAGGAAGGACCTAGAAAA
  ACTGGTCCGGAAGCTGTTGAAGCAAACCGATGAAAGCATTCGCGCTGAATCCAGCAGCAAGACCTCGATT
  CTCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204115 representing NM_001005565
  Red=Cloning site Green=Tags(s)
  
  MNPEERVVTWLISLGVLESPKKTICDPEEFLKSSLKNGVVLCKLISRLLPGSVEKYCQEPQTEADCIDNI
  NDFLKGCATLQVEVFEPDDLYSGANFSKVLNTLLAVNKATEDQLSERPCGRSSSLSATTSSQTNPQAAVP
  STTPEQQSEEKAAEMTENGSHQLIVKARFNFKQTNEDELSVCKGDIIYVTRVEEGGWWEGTLNGRTGWFP
  SNYVREIKPSERPLSPKAVKGFDTAPLTKNYYTVVLQNILDTEKEYAKELQSLLVTYLRPLQSNNNLSTV
  EFTCLLGNFEEVCTFQQTLCQALEECSKSPENQHKVGGCLLNLMPHFKSMYLAYCANHPSAVNVLTQHSD
  DLERFMENQGASSPGILILTTSLSKPFMRLEKYVTLLQELERHMEDTHPDHQDILKAIIAFKSLMGQCQD
  LRKRKQLELQILSEPIQAWEGDDIKTLGNVIFMSQVVMQHGACEEKEERYFLLFSSVLIMLSASPRMSGF
  MYQGKVPIAGMVVTRLDEIEGNDCTFEITGSTVERIVVHCNNNQDFQEWMEQLNRLTKGPASCSSLSKTS
  SSSCSTHSSFSSTGQPRGPLEPPQIIKPWSLSCLRPAPPLRPSAALGYKERMSYILKESSKSPKTMKKFL
  HKRKTERKTSEEEYVIRKSTAALEEDAQILKVIEAYCTSASFQQGTRKDSVPQVLLPEEEKLIIEETRSN
  GQTIIEEKSLVDTVYALKDEVKELKQENKKMKQCLEEELKSRKDLEKLVRKLLKQTDESIRAESSSKTSI
  LQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005565
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005565.1, NP_001005565.1
• RefSeq Size: 2319 bp
• RefSeq ORF: 2319 bp
• Locus ID: 363509
• Cytogenetics: Xq37
• MW: 87 kDa