Cyp7b1 (NM_019138) Rat Tagged ORF Clone

CAT#: RR204118

Cyp7b1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp7b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204118 representing NM_019138
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGAGCCACGACCCCAGATGCCGCTTCGCCCGGGCCTCTCTCCCTACTAGGCCTTCTCTTTGCGG
  TCACCTTGCTGCTCCCAGTCCTGTTCCTCCTCACCCGGCGCACAAGGCGTCCTTGTGAACCTCCCTTGAT
  AAAAGGTTGGATTCCTTATCTTGGCATGGCCCTGAAATTCTGGAAGGATCCGTTAGCTTTCTTGCAAACT
  CTTCAAAGGCAATGTGGTGACACTTTCACTGTCTTACTTGGAGGGAAGTATATAACATTTGTTCTGAACC
  CTTTCCAGTACCAGTATGTAATGAAAAACCCAAAACAATTAAGCTTTGAGAAGTTCAGCCGAAGATTATC
  AGCGAAAGCCTTCTCTGTCAAGAAGCTGCTAACTAATGACGACCTTAGCAATGACATTCACAGAGGCTAT
  CTTCTTTTACAAGGCAAATCTCTGGATGGTCTTCTGGAAACCATGATCCAAGAAGTAAAAGAAATATTTG
  AGTCCAGACTGCTAAAACTCACAGATTGGAATACAGCAAGAGTATTTGATTTCTGTAGTTCACTGGTATT
  TGAAATCACATTTACAACTATATATGGAAAAATTCTTGCTGCTAACAAAAAACAAATTATCAGTGAGCTG
  AGGGATGATTTTTTAAAATTTGATGACCATTTCCCATACTTAGTATCTGACATACCTATTCAGCTTCTAA
  GAAATGCAGAATTTATGCAGAAGAAAATTATAAAATGTCTCACACCAGAAAAAGTAGCTCAGATGCAAAG
  ACGGTCAGAAATTGTTCAGGAGAGGCAGGAGATGCTGAAAAAATACTACGGGCATGAAGAGTTTGAAATA
  GGAGCACATCATCTTGGCTTGCTCTGGGCCTCTCTAGCAAACACCATTCCAGCTATGTTCTGGGCAATGT
  ATTATCTTCTTCAGCATCCAGAAGCTATGGAAGTCCTGCGTGACGAAATTGACAGCTTCCTGCAGTCAAC
  AGGTCAAAAGAAAGGACCTGGAATTTCTGTCCACTTCACCAGAGAACAATTGGACAGCTTGGTCTGCCTG
  GAAAGCGCTATTCTTGAGGTTCTGAGGTTGTGCTCCTACTCCAGCATCATCCGTGAAGTGCAAGAGGATA
  TGGATTTCAGCTCAGAGAGTAGGAGCTACCGTCTGCGGAAAGGAGACTTTGTAGCTGTCTTTCCTCCAAT
  GATACACAATGACCCAGAAGTCTTCGATGCTCCAAAGGACTTTAGGTTTGATCGCTTCGTAGAAGATGGT
  AAGAAGAAAACAACGTTTTTCAAAGGAGGAAAAAAGCTGAAGAGTTACATTATACCATTTGGACTTGGAA
  CAAGCAAATGTCCAGGCAGATACTTTGCAATTAATGAAATGAAGCTACTAGTGATTATACTTTTAACTTA
  TTTTGATTTAGAAGTCATTGACACTAAGCCTATAGGACTAAACCACAGTCGCATGTTTCTGGGCATTCAG
  CATCCAGACTCTGACATCTCATTTAGGTACAAGGCAAAATCTTGGAGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204118 representing NM_019138
  Red=Cloning site Green=Tags(s)
  
  MEGATTPDAASPGPLSLLGLLFAVTLLLPVLFLLTRRTRRPCEPPLIKGWIPYLGMALKFWKDPLAFLQT
  LQRQCGDTFTVLLGGKYITFVLNPFQYQYVMKNPKQLSFEKFSRRLSAKAFSVKKLLTNDDLSNDIHRGY
  LLLQGKSLDGLLETMIQEVKEIFESRLLKLTDWNTARVFDFCSSLVFEITFTTIYGKILAANKKQIISEL
  RDDFLKFDDHFPYLVSDIPIQLLRNAEFMQKKIIKCLTPEKVAQMQRRSEIVQERQEMLKKYYGHEEFEI
  GAHHLGLLWASLANTIPAMFWAMYYLLQHPEAMEVLRDEIDSFLQSTGQKKGPGISVHFTREQLDSLVCL
  ESAILEVLRLCSYSSIIREVQEDMDFSSESRSYRLRKGDFVAVFPPMIHNDPEVFDAPKDFRFDRFVEDG
  KKKTTFFKGGKKLKSYIIPFGLGTSKCPGRYFAINEMKLLVIILLTYFDLEVIDTKPIGLNHSRMFLGIQ
  HPDSDISFRYKAKSWRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019138
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019138.1, NP_062011.1
• RefSeq Size: 2626
• RefSeq ORF: 1524
• Locus ID: 25429
• MW: 58.6 kDa
• Gene Summary: member of the p450 subfamily of cholesterol metabolizing enzymes that performs 7alpha-hydroxylation of 27-hydroxycholesterol in a bile synthesis pathway alternative to the classical cholesterol disposal pathway [RGD, Feb 2006]