Git1 (NM_031814) Rat Tagged ORF Clone

CAT#: RR204123

Git1 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor kinase interacting ArfGAP 1 (Git1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Git1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204123 representing NM_031814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGAAGGGGCCGCGAGCGGAGGTGTGTGCGGACTGCAGCGCCCCTGACCCTGGCTGGGCATCTA
  TCAGCAGAGGTGTGCTGGTTTGTGACGAGTGCTGCAGTGTGCACCGGAGCCTGGGACGACACATCTCCAT
  TGTCAAACACCTTCGCCACAGCGCCTGGCCTCCTACGCTGCTACAGATGGTGCACACGCTTGCCAGCAAC
  GGGGCCAACTCTATCTGGGAGCATTCCCTGCTGGACCCTGCACAAGTGCAAAGTGGCCGGCGCAAAGCCA
  ACCCCCAAGACAAAGTCCACCCCATCAAGTCAGAGTTCATCAGGGCAAAGTACCAGATGCTGGCGTTTGT
  GCACAAGCTTCCCTGCCGTGATGATGATGGGGTCACCGCCAAAGACCTCAGCAAGCAACTGCACTCGAGT
  GTGCGAACAGGCAACCTGGAGACCTGTCTGCGCCTGCTTTCCCTGGGTGCCCAGGCCAACTTCTTCCACC
  CAGAAAAGGGCACTACACCTCTACACGTGGCTGCCAAGGCAGGGCAGACACTGCAGGCTGAGCTGCTAGT
  AGTGTATGGGGCTGACCCAGGCTCCCCTGATGTCAATGGCCGCACACCCATTGACTATGCCAGGCAGGCG
  GGGCACCATGAACTGGCAGAAAGGCTAGTTGAGTGCCAGTATGAGCTCACTGACCGGTTGGCCTTCTACC
  TCTGTGGACGGAAGCCTGATCACAAGAATGGGCATTACATCATACCACAGATGGCTGACAGATCTCGGCA
  AAAGTGCATGTCTCAGAGTTTGGATCTGTCTGAATTGGCCAAAGCTGCCAAGAAGAAGCTACAAGCACTC
  AGCAACCGGCTCTTTGAGGAGCTCGCCATGGATGTGTATGACGAAGTGGACCGGAGAGAAAATGATGCAG
  TGTGGCTAGCTACCCAGAATCATAGCACCCTGGTGACAGAACGCAGTGCTGTGCCCTTCCTGCCAGTCAA
  CCCTGAATACTCAGCCACTCGGAATCAGGGACGACAGAAATTGGCTCGTTTTAATGCCCGAGAGTTTGCC
  ACCTTGATCATCGACATCCTCAGTGAGGCCAAACGGAGGCAGCAGGGCAAGAGTCTGAGCAGCCCCACAG
  ACAACCTCGAGCTGTCTGCACGGAACCAAAGTGACCTGGACGACCAGCACGACTACGATAGTGTGGCTTC
  TGACGAAGACACAGACCAGGAGCCCTTGCCCAGTGCTGGCGCCACGCGGAACAATCGTGCCAGGAGCATG
  GACTCCTCAGATCTGTCGGATGGGGCTGTGACGCTGCAGGAGTACCTGGAGCTGAAGAAGGCTCTGGCTA
  CCTCCGAGGCCAAAGTGCAGCAGCTCATGAAAGTCAACAGCAGTCTGAGTGACGAGCTTCGGAAGCTGCA
  GAGGGAGATCCACAAACTGCAGGCAGAGAACCTGCAGCTCCGGCAGCCACCAGGACCAGTGCCTGTACCC
  TCACTTCCCAGTGAACGGGCAGAACACACACTCATGGGCCCTGGTGGAAGTACCCATCGCAGGGACCGCC
  AGGCCTTCTCTATGTATGAGCCAGGCTCCGCCCTGAAGCCCTTTGGAGGTGCACCTGGGGATGAGCTCGC
  CACACGGCTCCAGCCTTTCCACAGCACAGAGCTGGAAGATGATGCCATCTATTCAGTACATGTCCCTGCT
  GGCCTTTACCGGATCCGGAAGGGGGTGTCTGCCTCTTCTGTGACCTTCACTCCCTCCTCCCCACTGCTGT
  CAAGCTCCCAAGAAGGAAGTCGCCATGCGAGCAAGCTTTCACGCCACGGCAGTGGCGCAGAGAGTGACTA
  TGAGAACACACAGAGTGGAGAGCCTCTGCTGGGACTTGAAGGGAAGCGATTCCTAGAGCTGAGCAAGGAG
  GATGAGCTGCACGCTGAGCTGGAGAGCTTAGATGGAGACCCAGACCCCGGGCTCCCCAGCACAGAAGATG
  TCATCCTAAAGACAGAGCAGGTCACCAAGAACATTCAGGAGCTATTGCGGGCAGCCCAGGAGTTCAAACA
  TGACAGCTTTGTGCCCTGTTCAGAAAAGATCCATTTGGCTGTGACTGAGATGGCCTCTCTCTTCCCAAAG
  AGGCCAGCCCTGGAGCCTGTGCGCAGTTCACTGCGGCTGCTCAACGCCAGCGCCTACCGGCTGCAGAGTG
  AATGCCGGAAGACAGTGCCTCCAGAACCTGGCGCCCCTGTGGACTTCCAGCTACTGACTCAGCAGGTGAT
  CCAGTGCGCCTATGACATCGCCAAGGCTGCCAAGCAGCTGGTCACCATCACCACCCGAGAGAAGAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204123 representing NM_031814
  Red=Cloning site Green=Tags(s)
  
  MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASN
  GANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSS
  VRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQA
  GHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQAL
  SNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFA
  TLIIDILSEAKRRQQGKSLSSPTDNLELSARNQSDLDDQHDYDSVASDEDTDQEPLPSAGATRNNRARSM
  DSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRKLQREIHKLQAENLQLRQPPGPVPVP
  SLPSERAEHTLMGPGGSTHRRDRQAFSMYEPGSALKPFGGAPGDELATRLQPFHSTELEDDAIYSVHVPA
  GLYRIRKGVSASSVTFTPSSPLLSSSQEGSRHASKLSRHGSGAESDYENTQSGEPLLGLEGKRFLELSKE
  DELHAELESLDGDPDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPK
  RPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031814
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031814.1, NP_114002.1
• RefSeq Size: 3236
• RefSeq ORF: 2313
• Locus ID: 83709
• MW: 85.2 kDa
• Gene Summary: mediates beta2-adrenergic receptor endocytosis [RGD, Feb 2006]