Acadsb (NM_013084) Rat Tagged ORF Clone

CAT#: RR204129

Acadsb (Myc-DDK-tagged ORF) - Rat acyl-Coenzyme A dehydrogenase, short/branched chain (Acadsb), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acadsb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204129 representing NM_013084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGTCTGCCTTCCAGCTGTGGCGTGCGGGCGGGCTGCTAAGAAGAAACTTCCTGACTCACTCGT
  CTTCTTGGAAAATCCCTCCTCGTGTCCTCAAATCCTCACAACCAGAAGCTCTACTCAGTGTAACAAATAA
  TGCCCTATGTTTCGCACCTCTGCAGACATTTACTGATGAGGATATTATGATGCAGAAGGCAGTCAAAAAA
  TTTGCACAGGAACAAATTGCTCCTTTGGTTTCAACTATGGATGAAAATTCAAAAATGGAGAAATCAGTGA
  TACAAGGATTATTCCAACAAGGGATGATGGGCATTGAAGTTGAAGCAAAATATGGAGGAACAGAAGCCTC
  CTTCTTGTGCTCTGTCCTGGTGATAGAGGAACTAGCTAAGGTGGATGCGTCCGTGGCTCTCCTATGTGAC
  ATCCAGAACACAGTCATTAACAAACTGTTTAGGAAGCACGGGACAGAAGAACAGAAGGCCACCTATTTGC
  CAAAGCTGGTTACGGAAAAATTAGGGAGTTTTTGCCTCTCTGAAGCTGGAGCTGGTAGCGACTCTTTTGC
  TTTGAAAACAAGAGCTGATAAAAGTGGAAATTACTACGTCATCAATGGATCGAAGATGTGGATCAGCAAT
  GCTGAACATGCAGAGCTCTTCCTGGTTTTTGCAAATGTGGACCCCCCCTCTGGGTACAGAGGCATCACCT
  GCTTCTTAGTAGACCGAGATACAGAAGGTTTTCAAATAGGGAGACGAGAAAACAAAATGGGGATCAGAGC
  TTCTTCCACCTGTCAGTTAACATTTGAAAATGTCAAGGTTCCAGAAACCAGTGTTTTGGGGAAAATTGGG
  CACGGTTATAAATATGCCATAGGAAGTCTTAACGAAGGTAGAATTGGAATTGCTGCCCAGATGCTAGGAC
  TGGCCCAAGGATGTTTTGACTACACTATTCCATACATAAAAGAAAGGATGCAGTTCGGCAAACGAATATT
  TGATTTTCAGGGGCTCCAACACCAAGTGGCTCACGTGGCCACCCAGCTGGAAGCTGCGCGGTTGCTGACG
  TACAACGCTGCTAGGCTTGTAGAAGCCGGGAGGCCATTTATAAAAGAAGCATCTATGGCCAAGTATTATG
  CATCAGAGGTCGCTGGGCTGACAACAAGCAAGTGCATCGAGTGGATGGGAGGGGTCGGCTACACCAAAGA
  TTACCCTGTGGAGAAATTCTTCCGCGATGCGAAGATCGGTACAATATATGAAGGAACTTCCAACATCCAG
  CTGAACACCATTGCAAAGCACATCGATGCAGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204129 representing NM_013084
  Red=Cloning site Green=Tags(s)
  
  MAVSAFQLWRAGGLLRRNFLTHSSSWKIPPRVLKSSQPEALLSVTNNALCFAPLQTFTDEDIMMQKAVKK
  FAQEQIAPLVSTMDENSKMEKSVIQGLFQQGMMGIEVEAKYGGTEASFLCSVLVIEELAKVDASVALLCD
  IQNTVINKLFRKHGTEEQKATYLPKLVTEKLGSFCLSEAGAGSDSFALKTRADKSGNYYVINGSKMWISN
  AEHAELFLVFANVDPPSGYRGITCFLVDRDTEGFQIGRRENKMGIRASSTCQLTFENVKVPETSVLGKIG
  HGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERMQFGKRIFDFQGLQHQVAHVATQLEAARLLT
  YNAARLVEAGRPFIKEASMAKYYASEVAGLTTSKCIEWMGGVGYTKDYPVEKFFRDAKIGTIYEGTSNIQ
  LNTIAKHIDAEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013084
• ORF Size: 1296 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013084.1, NM_013084.2, NP_037216.1
• RefSeq Size: 1321
• RefSeq ORF: 1299
• Locus ID: 25618
• MW: 47.8 kDa
• Gene Summary: catalyzes the alpha, beta-dehydrogenation of valproyl-CoA and other acyl-CoA esters in fatty acid metabolism [RGD, Feb 2006]