Veph1 (NM_001014171) Rat Tagged ORF Clone

CAT#: RR204146

Veph1 (Myc-DDK-tagged ORF) - Rat ventricular zone expressed PH domain homolog 1 (zebrafish) (Veph1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Veph1
• Synonyms: RGD1308217
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204146 representing NM_001014171
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCAACTTTTCAGATTGGTTTTGGGACAAAAAGATCTTTCAAGAGCTGGGGACCTCTTCTCTTTAG
  ATGACGCTGAGATAGAAGACAGCCTGACAGAAGCTTTGGAGCAGATTAAAGTAATTAGCTCATCTTTGGA
  TTATCAAACTAATAACAATGACCAGGCAGTGGTTGAAATCTGTATCACGAGGATTACAACAGCCATCAGA
  GAGACGGAATCCATTGAAAAGCACGCCAGGGCCCTGGTGGGACTGTGGGATTCCTGCTTGGAGCACAACC
  TGAGACCTGCAGGGAAAGACGAAGATACTCCACACGCTAAAATCGCATCCGATATCATGAGTTGTATCTT
  ACAAAATTACAACCGGACCCCTGTGATGGTCTTAGCTGTACCCATCGCAGTGAAATTCCTCCACAGAGGC
  AGCAAGGAGCTGTGTAGGAACATGTCCAACTACCTGTCCTTGGCTGCCATCACCAAGGCCGATCTCCTGG
  CTGACCACACTGAAGGGATAGTAAAGAGCATACTCCAAGGAAATGCCATGCTACTGAGGGTGTTGCCTGC
  TGTGTATGAAAAGCAGCCCCAACCAATCAACAGGCACCTGGCAGAACTCCTGGCCTTGATGTCTCAATTG
  GAGCAGACAGAACAGTACCATCTACTACGGCTTCTCCATGTAGCAGCAAAGAGAAAAGATGTGGAGGTGG
  TTCAGAAATGTGTTCCTTTCCTGATCAGAAACCTGAAGGAGTCAACCTATAATGACATCATCCTGAACAT
  CCTCATAGAAATAGCAGGCCATGAGCCGCTGGCCTTGAACAGTTTTCTCCCAATGCTGAAAGAGATTGCA
  GAGCAATTCCCCTACCTCACTGGACAAATGGCGAGGATCTTTGGTGCTGTTGGGCATGTGGATGAAGAGA
  GAGCCAGGAGCTGCCTACGGTACCTGGTGAGCCAGCTGGCTAACATGGAGCATTCATTCCACCACATTCT
  CCTGCTGGAAATTAAGAGTCTCACAGATGCCTTCTCCTCCATTCTGGGCCCTCACAGCAGGGACATCTTC
  CGAATGAGCAACAGCTTCACCAACATCGCCAAGCTGCTTTCCCGACAACTGGAAAACAGCAAGGCCGAGA
  GTGGCAGGAGAAGAACCGGCACTGAAGTTAGCTTTCCTGAGAAATTCAGAGAACCCAAGACCATGGAGCC
  TAAAAGTGAAGACCACGAAAAACTCCAAGTTAAAATCCAGGCCTTCGAAGATAAAATAAATGCTGAGAGC
  CATACCCCTGGCTCTGTCCGGAGATACAGTTTAGACCACGTCTCCAAGGAAGAACGAAAAAACGTTCGAT
  TTAGCAGGTCCAGAAGTTTGGCTTTGAATACTGTGCTCACAAACAGTGTGAATGTGGAAGACGGAGAAAT
  CGAAGGAAAGACAGGAATGCATGCCAGCATCTCGCTTTCAGAAATAGACCCACCTGGCCACGGAATTGGC
  AAGGTGCCCTTTAAGACGGATACCCATGGATCACAGCTGAGAAATTCCTCAGCTTCACACCCAAGTATTA
  TACACAGTGAACCAGAAAATATGCCTGAAACTTTTAAAGAAAACGTCCAAGAAGAAATTCCAGAGGCGGC
  CATAAGTCCTGTGGAATACCAAGATAAGCTCTACTTACACTTAAAGGAAAACCTCAGCAAAGTGAAGGCA
  TACGCCTTGGAAATCGCAAAAAAAGTCCCAATCCCAGACCAGTGCACCATTGAAGATAATACGAGAAGCT
  GTGTGGCAAAGCTGTTCTTTACGTGTTCGTTAAAGGGTCATTACTGCCTATACAGCAAGTCCAGTTTCAC
  CCTTGTCAGCCAAGCCCCCCAGCCATGGATTCAGATCATGTTTCTCTTCCAACAGAGCCTGTTTCCTGAG
  CCCCTGTCCATTCAGAGTGGCTCGGTGCAGTTCCTCAAGGCCCTATGGGAGAAGACACAGGGCACAGGAA
  CCCACAGCTTTGAGGTCGCCATGACAGAGTCCACATTTCCACAGCAGAAGGATCTGGACCAGCTTCAGCT
  CCATCTGGAAGAAGTGCGGTTCTTTGATGTGTTTGGCTTCAGCGAAACAGCAGGGGCATGGCAATGCTTC
  ATGTGTAACAACCCCGAAAAGGCAACTGTTATAAATCAAGATGGCCAGCCTCTCATTGAAGGAAAGCTTA
  AGGAGAAACAAGTGAGGTGGAAGTTCATCAAGAGGTGGAAAACACGTTATTTTACACTGGCTGGAAACCA
  ACTTCTGTTTCAAAAGGGGAAATCTGACGACCCCGATGATTCCCCGATAGAACTCAGCAAAGTACAGAGC
  GTGAAAGCTGTGGCCAAGAAGCGTCGGGACCGCTCTCTCCCTCGGGCTTTTGAAATCTTCACAGACAATA
  AGACCTATGTTTTCAAAGCCAAGGATGAGAAAAACGCAGAGGAGTGGCTGCAGTGCATCAACGTGGCGCT
  GGCCCAGGCAAAGGAGAGGGAGAGCCGAGAAGTAACCACATATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204146 representing NM_001014171
  Red=Cloning site Green=Tags(s)
  
  MHQLFRLVLGQKDLSRAGDLFSLDDAEIEDSLTEALEQIKVISSSLDYQTNNNDQAVVEICITRITTAIR
  ETESIEKHARALVGLWDSCLEHNLRPAGKDEDTPHAKIASDIMSCILQNYNRTPVMVLAVPIAVKFLHRG
  SKELCRNMSNYLSLAAITKADLLADHTEGIVKSILQGNAMLLRVLPAVYEKQPQPINRHLAELLALMSQL
  EQTEQYHLLRLLHVAAKRKDVEVVQKCVPFLIRNLKESTYNDIILNILIEIAGHEPLALNSFLPMLKEIA
  EQFPYLTGQMARIFGAVGHVDEERARSCLRYLVSQLANMEHSFHHILLLEIKSLTDAFSSILGPHSRDIF
  RMSNSFTNIAKLLSRQLENSKAESGRRRTGTEVSFPEKFREPKTMEPKSEDHEKLQVKIQAFEDKINAES
  HTPGSVRRYSLDHVSKEERKNVRFSRSRSLALNTVLTNSVNVEDGEIEGKTGMHASISLSEIDPPGHGIG
  KVPFKTDTHGSQLRNSSASHPSIIHSEPENMPETFKENVQEEIPEAAISPVEYQDKLYLHLKENLSKVKA
  YALEIAKKVPIPDQCTIEDNTRSCVAKLFFTCSLKGHYCLYSKSSFTLVSQAPQPWIQIMFLFQQSLFPE
  PLSIQSGSVQFLKALWEKTQGTGTHSFEVAMTESTFPQQKDLDQLQLHLEEVRFFDVFGFSETAGAWQCF
  MCNNPEKATVINQDGQPLIEGKLKEKQVRWKFIKRWKTRYFTLAGNQLLFQKGKSDDPDDSPIELSKVQS
  VKAVAKKRRDRSLPRAFEIFTDNKTYVFKAKDEKNAEEWLQCINVALAQAKERESREVTTYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014171
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014171.1, NP_001014193.1
• RefSeq Size: 3068 bp
• RefSeq ORF: 2499 bp
• Locus ID: 361954
• Cytogenetics: 2q31
• MW: 94.5 kDa