Idh1 (NM_031510) Rat Tagged ORF Clone

CAT#: RR204198

Idh1 (Myc-DDK-tagged ORF) - Rat isocitrate dehydrogenase 1 (NADP+), soluble (Idh1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Idh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204198 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGAAAAATCCATGGCGGTTCTGTGGTGGAAATGCAAGGAGATGAAATGACACGGATCATCTGGG
  AATTGATTAAAGAAAAGCTCATTCTTCCCTACGTGGAACTGGATCTGCATAGCTATGACTTAGGCATAGA
  GAATCGGGATGCCACCAATGACCAGGTCACCAAGGATGCTGCGGAGGCTATAAAGAAATACAACGTCGGC
  GTCAAGTGTGCTACCATCACCCCCGATGAGAAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGGAAAT
  CACCCAACGGTACCATCCGGAACATTCTGGGCGGCACTGTCTTCAGAGAAGCTATTATCTGCAAAAATAT
  CCCCCGGCTAGTGACAGGCTGGGTAAAACCCATCATCATTGGCCGACACGCCTATGGGGACCAATACAGA
  GCAACAGATTTCGTTGTTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAAAGATGGGTCGCAGA
  AAGTGACATACCTGGTGCATGACTTTGAAGAAGGTGGTGGTGTTGCCATGGGTATGTACAACCAGGATAA
  GTCAATTGAAGATTTTGCACACAGTTCCTTCCAAATGGCTCTGTCGAAGGGCTGGCCTTTGTATCTAAGC
  ACCAAGAACACTATTCTGAAGAAGTATGATGGACGCTTCAAAGACATCTTCCAGGAGATCTATGACAAGC
  AATACAAGTCCAAGTTTGAAGCTCAGAAGATCTGGTATGAACACAGGCTCATAGATGACATGGTGGCCCA
  GGCTATGAAATCAGAAGGAGGCTTCATCTGGGCCTGTAAGAACTATGATGGTGATGTGCAGTCAGACTCA
  GTAGCCCAAGGTTATGGCTCCCTTGGCATGATGACCAGTGTGCTGATTTGTCCAGATGGTAAGACGGTAG
  AAGCAGAGGCTGCCCATGGCACTGTCACACGTCACTACCGCATGTACCAGAAAGGACAGGAGACGTCCAC
  CAATCCCATTGCTTCCATTTTTGCCTGGTCCCGAGGGTTAGCCCACAGAGCAAAGCTTGACAACAATACT
  GAGCTCAGCTTCTTTGCAAATGCTTTGGAAGAAGTCTGCATTGAGACCATTGAGGCTGGCTTTATGACTA
  AGGACTTGGCTGCTTGCATTAAAGGCTTACCCAATGTACAGCGTTCTGACTACTTGAATACATTTGAGTT
  TATGGACAAACTTGGAGAAAACTTGAAGGCCAAACTAGCTCAGGCCAAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204198 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRKIHGGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVG
  VKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYR
  ATDFVVPGPGKVEITYTPKDGSQKVTYLVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLS
  TKNTILKKYDGRFKDIFQEIYDKQYKSKFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDS
  VAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNT
  ELSFFANALEEVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKAKLAQAKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031510
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031510.1, NP_113698.1
• RefSeq Size: 1719
• RefSeq ORF: 1245
• Locus ID: 24479
• MW: 46.7 kDa
• Gene Summary: catalyzes oxidative decarboxylation of citrate; cytosolic NADP+-dependent isozyme [RGD, Feb 2006]