Crh (NM_031019) Rat Tagged ORF Clone

CAT#: RR204203

Crh (Myc-DDK-tagged ORF) - Rat corticotropin releasing hormone (Crh), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crh
• Synonyms: CRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204203 representing NM_031019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTGCGGCTGCTGGTGTCCGCGGGCATGCTGCTGGTGGCTCTGTCGCCCTGTCTGCCTTGCAGGG
  CCCTGCTGAGCAGGGGATCCGTCTCTGGAGCGCCGCGGGCCCCGCAGCCGTTGAATTTCTTGCAACCGGA
  GCAGCCCCAGCAACCTCAGCCGATTCTGATCCGCATGGGTGAAGAATACTTCCTCCGCCTGGGGAACCTC
  AACAGAAGTCCCGCTGCTCGGCTGTCCCCCAACTCCACGCCCCTCACCGCGGGTCGCGGCAGCCGCCCCT
  CGCACGACCAGGCTGCGGCTAACTTTTTCCGCGTGTTGCTGCAGCAGCTGCAGATGCCTCAGCGCCCGCT
  CGACAGCAGCACGGAGCTGGCGGAACGCGGCGCCGAGGATGCCCTCGGTGGCCACCAGGGGGCGCTGGAG
  AGGGAGAGGCGGTCCGAGGAGCCGCCCATCTCTCTGGATCTCACCTTCCACCTTCTGAGGGAAGTCTTGG
  AAATGGCCAGGGCAGAGCAGTTAGCTCAGCAAGCTCACAGCAACAGGAAACTGATGGAGATTATCGGGAA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204203 representing NM_031019
  Red=Cloning site Green=Tags(s)
  
  MRLRLLVSAGMLLVALSPCLPCRALLSRGSVSGAPRAPQPLNFLQPEQPQQPQPILIRMGEEYFLRLGNL
  NRSPAARLSPNSTPLTAGRGSRPSHDQAAANFFRVLLQQLQMPQRPLDSSTELAERGAEDALGGHQGALE
  RERRSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031019
• ORF Size: 561 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031019.1, NP_112281.1
• RefSeq Size: 1197
• RefSeq ORF: 564
• Locus ID: 81648
• MW: 20.7 kDa
• Gene Summary: binds CRH receptors and mediates neuroendocrine stress response; may play a role in the consolidation of long term memory [RGD, Feb 2006]