Numbl (NM_001033888) Rat Tagged ORF Clone

CAT#: RR204216

Numbl (Myc-DDK-tagged ORF) - Rat numb homolog (Drosophila)-like (Numbl), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Numbl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204216 representing NM_001033888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGCAGCGCGGCGGCCAGCGGCGGACCCAGGAGACCTGATCAGCATTTGCCCCCAGCCCCCTGTG
  GGGCCTCGGGGCCCCCTGAAACCTTCAGGACGGAGTCAGACGGGGCGGGCACCATGAACAAGTTACGGCA
  GAGTCTGCGGCGGAGGAAGCCAGCCTATGTGCCTGAGGCGTCACGCCCACACCAGTGGCAGGCAGATGAG
  GATGCGGTGCGGAAGGGCACATGCAGCTTCCCCGTTAGGTACCTGGGCCACGTGGAAGTGGAGGAGTCCC
  GGGGGATGCACGTTTGTGAAGACGCGGTGAAGAAGCTGAAGGCGATGGGCCGGAAGTCCGTGAAGTCTGT
  CCTGTGGGTGTCGGCTGATGGGCTCCGAGTGGTGGATGACAAGACCAAGGACCTGCTGGTAGACCAGACC
  ATCGAGAAGGTGTCCTTCTGTGCTCCTGACCGCAACCTGGACAAGGCATTCTCCTACATATGCCGTGATG
  GCACCACACGCCGCTGGATCTGCCACTGTTTCCTGGCCCTCAAGGACTCTGGTGAGAGGCTGAGCCACGC
  TGTGGGCTGCGCGTTCGCTGCCTGCCTGGAGAGGAAGCAGCGACGGGAGAAGGAGTGCGGAGTCACCGCC
  GCCTTCGATGCCAGTCGCACCAGCTTTGCCCGCGAGGGCTCCTTCCGCCTGTCCGGTGGCGGGCGGCCTG
  CGGAGCGCGAGGCTGGGGACAAGAAGAAAGAAGCAGCAGCTGCTCCTGCTGTGGCTCCTGGCCCTGCCCA
  GCCTGGGCACGTTTCTCCGACACCGGCCACTACATCCCCTGGTGAGAAGGGAGAGGCGGGTACCCCAGTG
  GCTGCAGGCACCACTGCTGCTGCCATTCCCCGGCGCCATGCACCTCTGGAACAGCTGGTTCGCCAGGGCT
  CCTTTCGTGGGTTTCCTGCCCTCAGCCAGAAGAACTCACCTTTCAAACGTCAGCTGAGTCTACGGTTGAA
  TGAGCTGCCATCCACACTGCAGCGCCGTACAGACTTCCAGGTGAAGGGCACAGTGCCTGAGATGGAGCCT
  CCTGGTACTGGTGACAGCGACGGCATCAGTGCCCTGTGTACACAAATCAGCTCGTCTTTCGCCAGTGCCG
  GAGCACCAGTGCCAGGGCCACCGTCTGCCACCACAGGGACGTCTGCCTGGGGTGAGCCCTCTGTACCTGC
  TGCAACTGCCTTCCAGCCCGGGCACAAGCGGACACCGTCGGAGGCTGAGCGATGGTTGGAGGAAGTGTCC
  CAGGTGGCTAAGGCCCAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
  AGCAGCAGCAGCAGCAACAACAACAGCAACAAGCCACCTCCGTGCCACCAATGCCCACCATGGCCCCCAC
  CCTTCAACCCTTCTCCGCCCCAGTGGGGCCCTTTGACACTGCAGCTGCCCAGGTGGCTGTGTTCCTGCCA
  CCCACACACATGCAGCCTCCATTTGTGCCCGCCTACCCAGGCCTGGGTTACCCACCCATGCCCCGGGTGC
  CAGTGGTGGGCATCACACCTTCACAGATGGTGGCCAACGCCTTCTGCTCAGCTGCTCAGCTCCAGCCCCA
  ACCTGCCACACTGCTTGGAAAAGCCGGGGCCTTCCCCCCACCTACCGCACCCAGTGCCCCTGGGGGCCAG
  GCCCGTCCACGCCCTAATGGGGCTCCCTGGCCCCCAGAGCCAGCGCCTGCCCCTGCCCCTGAGTTGGACC
  CCTTTGAGGCCCAGTGGGCAGCTTTAGAAGGCAAGCCCGCCGTGGAGAAACCCTCCAACCCCTTCTCTGG
  TGACTTGCAGAAGACCTTCGAGATTGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204216 representing NM_001033888
  Red=Cloning site Green=Tags(s)
  
  MSRSAAASGGPRRPDQHLPPAPCGASGPPETFRTESDGAGTMNKLRQSLRRRKPAYVPEASRPHQWQADE
  DAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGRKSVKSVLWVSADGLRVVDDKTKDLLVDQT
  IEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSGERLSHAVGCAFAACLERKQRREKECGVTA
  AFDASRTSFAREGSFRLSGGGRPAEREAGDKKKEAAAAPAVAPGPAQPGHVSPTPATTSPGEKGEAGTPV
  AAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKRQLSLRLNELPSTLQRRTDFQVKGTVPEMEP
  PGTGDSDGISALCTQISSSFASAGAPVPGPPSATTGTSAWGEPSVPAATAFQPGHKRTPSEAERWLEEVS
  QVAKAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQATSVPPMPTMAPTLQPFSAPVGPFDTAAAQVAVFLP
  PTHMQPPFVPAYPGLGYPPMPRVPVVGITPSQMVANAFCSAAQLQPQPATLLGKAGAFPPPTAPSAPGGQ
  ARPRPNGAPWPPEPAPAPAPELDPFEAQWAALEGKPAVEKPSNPFSGDLQKTFEIEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033888
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033888.1, NP_001029060.1
• RefSeq Size: 1854 bp
• RefSeq ORF: 1854 bp
• Locus ID: 292732
• Cytogenetics: 1q21
• MW: 65.9 kDa