Irak2 (NM_001025422) Rat Tagged ORF Clone

CAT#: RR204289

Irak2 (Myc-DDK-tagged ORF) - Rat interleukin-1 receptor-associated kinase 2 (Irak2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Irak2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204289 representing NM_001025422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGCTACATCTACCAGCTGCCGTCCTGGGTACTGGACGACCTGTGCCGCAATATCGACACGCTCA
  GTGAGTGGGACTGGATGCAGTTCGCTTCCTATGTGATCACAGACCTGACACAGCTGAGGAAGATCAAGTC
  CATGGAGAGGGTTCAGGGGGTGAGCATCACCCGGGAGCTGCTGTGGTGGTGGTCCATGCGGCAGGCCACT
  GTCCAGCAGCTGGTGGACCTCCTGTGTCACCTGGAACTCTACCGAGCTGCCCAGATTGTCCTGAGCTGGA
  AGCCGGCTCCTGACAGCTTGTCTCCCCTCTCGGCCTTCCCAGAGGCTGTGAAGCCAGGTCCTGTGGCTAC
  TTCTGGAAGAAACCTCAAGGATGACCAGAAGAAGGGCCAGCCTGTGAAACCATGCTCCTTTCTGAGCTCA
  GGGACCACCATGGCTGGCGCCCAGCAGCAGGCCTCATGCCAACGTCCATGCGAGGAGGATGCCCCTTGTT
  CCCTGAAAACTGATGTACCTGACTCTCTCCAATCTAAGTATTGTAGCACGTCCATCCCCAAGCAGGAAAA
  GCTTTTGAATTTGCCCGGAGACAGGCTTTTCTGGAGTGAAGCAGATATCGTTCAAGCAACTGAGGACTTT
  GACCAAAGCCACCGAATCAGTGAGGGGACCTTTGCCGATATCTACCGGGGACAAAGGAATGGCGTGGCCT
  TTGCCTTCAAGAGGCTCAGGGAGGTGACTGGCTCAAGTCCAGGGTCCATGGACAGATTCCTGCAGGCGGA
  GATGCAGCTCTGCCTCAGATGCTGCCACCCCAACATCCTACCTCTGCTGGGCTTCTGCACTGGAAGACAG
  TTCCACAGCCTCATCTACCCCTACATGGCCAATGGCTCTCTGCAGGACAGACTCTGGGCTCAGGGCGACT
  CAGACATGTTGTCCTGGCCACAGCGTGCCAGCATCTGCTCAGGGCTTCTTCTAGCTGTGGAACACCTGCA
  TAGCCTGGATATCATCCACAGTAATGTGAAGAGTGCCAACGTTTTGCTGGACCAGCATCTTAACCCCAAG
  CTAGCCCATCCAGTAGCTCACCCGTGTCCCACCAATAAAAAGACCAAATATACTGTAATGAAGACTCACC
  TGTTCCAGGCCTCAGCTGCGTATCTGCCTGAAAACTTCATCCGAGTGGGACAGCTCACCAAGCAAGTAGA
  CATCTTCAGCTGTGGAATTGTATTGGCCGAGGTTCTCACCGGCATCCCTGCAATGGACAAGGACCGCAGT
  CCAGTTTACCTGAAGGATTTGCTTCTCAGTGAGATTCCCAGCAATACTTCCTCAGTCCACTCCAGGAAGA
  CCAGCATGGGGAAGGTGGTGGTGAAAGAGATCTGCCAGAAGCACTTGGAGAGGAAGGCAGGGCTACTGCC
  TGAGGCCTGCGCGGAAACCTGGGCCACCGCTGTGAGCGTCTGCCTGCGGAGACGGGAGGCCAGCCTGGAG
  GAGGCACGAGTCTCCATGGCTGGGGTAGAGGAGCAGCTCAGAGGGCAGCTGTCCCTCCCCTGGAGCAGGG
  TTTCTGAGGATACGGGCTCATCTTCTAATACCCCAGAGGAAACGGATGACGTTGACAATTCCAGCCTCAG
  TGTCCCCTCTTCTGTTATGGTGGTGTCATGTGCAAGGGTTTCCTCCCCTCCACCATCCATGGGAAATGGG
  ACAGCACAGCCATCTACCAGTGGGAGGCAGGAGGCTGACTCCTCCTCCGAGGCTTGTGCAGGCCCACAGC
  CACCTCAGGAAGCTACAGAGACTTCATGGAAAATTGAGATCAATGAGGCCAAAAGGAGACTGATGGAGAA
  CATCCTACTGTATAAAGAAGAGAAACTGGACAGCGTTGAGCTTTTTGGACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204289 representing NM_001025422
  Red=Cloning site Green=Tags(s)
  
  MACYIYQLPSWVLDDLCRNIDTLSEWDWMQFASYVITDLTQLRKIKSMERVQGVSITRELLWWWSMRQAT
  VQQLVDLLCHLELYRAAQIVLSWKPAPDSLSPLSAFPEAVKPGPVATSGRNLKDDQKKGQPVKPCSFLSS
  GTTMAGAQQQASCQRPCEEDAPCSLKTDVPDSLQSKYCSTSIPKQEKLLNLPGDRLFWSEADIVQATEDF
  DQSHRISEGTFADIYRGQRNGVAFAFKRLREVTGSSPGSMDRFLQAEMQLCLRCCHPNILPLLGFCTGRQ
  FHSLIYPYMANGSLQDRLWAQGDSDMLSWPQRASICSGLLLAVEHLHSLDIIHSNVKSANVLLDQHLNPK
  LAHPVAHPCPTNKKTKYTVMKTHLFQASAAYLPENFIRVGQLTKQVDIFSCGIVLAEVLTGIPAMDKDRS
  PVYLKDLLLSEIPSNTSSVHSRKTSMGKVVVKEICQKHLERKAGLLPEACAETWATAVSVCLRRREASLE
  EARVSMAGVEEQLRGQLSLPWSRVSEDTGSSSNTPEETDDVDNSSLSVPSSVMVVSCARVSSPPPSMGNG
  TAQPSTSGRQEADSSSEACAGPQPPQEATETSWKIEINEAKRRLMENILLYKEEKLDSVELFGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025422
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025422.1, NP_001020593.1
• RefSeq Size: 2977 bp
• RefSeq ORF: 1875 bp
• Locus ID: 362418
• Cytogenetics: 4q42
• MW: 69.2 kDa