Nars2 (NM_001034921) Rat Tagged ORF Clone

CAT#: RR204309

Nars2 (Myc-DDK-tagged ORF) - Rat asparaginyl-tRNA synthetase 2 (mitochondrial)(putative) (Nars2), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nars2
• Synonyms: RGD1305145
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204309 representing NM_001034921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGGGGCCCGGCGCCTGCTCGGAGCGCTGCGCCTCTGTTCCTCTGTTTCCTGCCCCAGGCCCCGCG
  CGACTGCCAAACTGCGCGTGCGGGACGCTCTTGGGGTCCAGGACGCGAGTGGGGAGTGCGTAACGGTGCA
  GGGGTGGATCCGGTCAGTCAGATCCCAGAAGGAAGTTTTGTTCCTGCATGTAAATGACGGCTCTTCTTTG
  GAAAGTCTTCAGGTTGTTGCAGATTCAAGCTTCGACAGTAGAGAATTGGCTTTTGGGAGTTCTGTGCAAG
  TCCAAGGGCAGCTGGTCAAAAGTCAGTCCAAGAGGCAAAATGTGGAACTGAAGGCAGAGAAGATTGAAGT
  TGTTGGAACCTGTGAAGCCAAGGCATTTCCTATAAAGTATAAGGAGAGGCATCCTCTGGAGTACCTGAGA
  CAGTACCCTCACCTGAGGTGTCGGACTAATGCTCTGGGTTCCATACTGAGAGTCCGCAGTGAAGCCACAG
  CTGCTATTCATTCCTACTTTAAGGACAACGGCTTCATGCACATCCATACCCCAGTCCTCACGTCCAATGA
  CTGTGAGGGGGCCGGAGAGCTCTTTCAAGTGGAGCCTTCAAGCAAAATAAAGGTACCCGAGGAGAATTTC
  TTTGATGTTCCTGCTTTCTTAACTGTCTCAGGACAACTTCACCTAGAAGTGATGTCAGGAGCTTTTACCC
  AAGTGTTTACCTTCGGCCCAACGTTCCGAGCTGAGAATTCGCAGAGCCGGAGACACCTGGCTGAATTCTA
  TATGGTCGAAGCCGAGGTTTCTTTTGTTGAAAGCCTTCAGGATCTCATGCAGGTCATGGAAGGACTCTTC
  AAGGCCACCACCGAGATGGTTCTCTCACACTGTCCTGAAGATGTTGAACTGTGTCACCAGTTCATAGCTT
  CTGGCCAGAAGGACAGACTAGAACATATGCTAAAAAACAACTTCCTAATCATCTCTTATGCAGAAGCAAT
  TGAGATCTTAAAGCAAGCATCCCAGAACTTCACCTTTACACCCAAGTGGGGTGTTGATCTACAAACTGAA
  CATGAGAAGTACCTGGTGAGACACTGTGGCAACATACCAGTCTTTGTCATTAACTATCCATCAGAACTCA
  AGCCCTTCTACATGCGGGAGAATGAAGATGATCCTCAGAACACAGAAGTAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204309 representing NM_001034921
  Red=Cloning site Green=Tags(s)
  
  MLGARRLLGALRLCSSVSCPRPRATAKLRVRDALGVQDASGECVTVQGWIRSVRSQKEVLFLHVNDGSSL
  ESLQVVADSSFDSRELAFGSSVQVQGQLVKSQSKRQNVELKAEKIEVVGTCEAKAFPIKYKERHPLEYLR
  QYPHLRCRTNALGSILRVRSEATAAIHSYFKDNGFMHIHTPVLTSNDCEGAGELFQVEPSSKIKVPEENF
  FDVPAFLTVSGQLHLEVMSGAFTQVFTFGPTFRAENSQSRRHLAEFYMVEAEVSFVESLQDLMQVMEGLF
  KATTEMVLSHCPEDVELCHQFIASGQKDRLEHMLKNNFLIISYAEAIEILKQASQNFTFTPKWGVDLQTE
  HEKYLVRHCGNIPVFVINYPSELKPFYMRENEDDPQNTEVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034921
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034921.1, NP_001030093.1
• RefSeq Size: 1785 bp
• RefSeq ORF: 1176 bp
• Locus ID: 293128
• Cytogenetics: 1q32
• MW: 44.2 kDa