Purb (NM_001017503) Rat Tagged ORF Clone

CAT#: RR204335

Purb (Myc-DDK-tagged ORF) - Rat purine rich element binding protein B (Purb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Purb
• Synonyms: pur-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204335 representing NM_001017503
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGCGACAGCGGCAGCGAGCGCGGTGGCGGCGGGCCCGGCAGCTTCCAGCCCGCGCCCCGCG
  GCGGCGGAGGGCCTGGCGGCGAGCAGGAGACGCAGGAGCTGGCCTCGAAGCGGCTGGACATCCAGAACAA
  GCGCTTCTACCTGGACGTGAAGCAGAACGCCAAGGGCCGCTTCCTCAAGATCGCCGAGGTGGGCGCGGGC
  GGCTCCAAGAGCCGCCTCACGCTCTCGATGGCGGTGGCCGCCGAGTTCCGCGACTCGCTGGGCGACTTCA
  TCGAGCACTACGCGCAGCTGGGTCCCAGCAGCCCGGAGCAGCTGGCGGCGGGCGCCGAGGAGGGCGGCGG
  GCCGCGCCGCGCGCTTAAGAGCGAATTCCTGGTGCGCGAGAACCGCAAGTACTACCTGGACCTCAAGGAG
  AACCAGCGCGGCCGCTTCCTGCGCATCCGTCAGACGGTAAACCGCGGCGGTGGAGGCTTCGGCGGGGGAC
  CCGGGCCCGGCGGCCTGCAGAGCGGCCAGACCATCGCGCTGCCCGCGCAGGGCCTCATCGAGTTCCGCGA
  CGCGCTCGCCAAGCTCATCGACGACTACGGGGGCGAGGATGACGAGCTGGCCGGTGGCCCGGGAGGCGGC
  GCCGGCGGCCCTGGGGGCGGCCTGTACGGAGAGCTCCCGGAGGGCACCTCCATCACGGTGGACTCCAAGC
  GCTTCTTCTTCGACGTCGGCTGCAACAAGTACGGGGTGTTCCTGCGAGTGAGCGAGGTGAAGCCATCCTA
  CCGCAATGCCATCACCGTGCCCTTCAAGGCCTGGGGCAAGTTTGGAGGCGCCTTCTGTCGGTATGCAGAT
  GAAATGAAAGAAATCCAGGAGCGACAGAGGGATAAGCTTTACGAGCGACGCGGTGGGGGCAGCGGCGGTG
  GCGACGAGTCCGAGGGTGAGGAAGTGGATGAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204335 representing NM_001017503
  Red=Cloning site Green=Tags(s)
  
  MADGDSGSERGGGGPGSFQPAPRGGGGPGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAG
  GSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKE
  NQRGRFLRIRQTVNRGGGGFGGGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGG
  AGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYAD
  EMKEIQERQRDKLYERRGGGSGGGDESEGEEVDED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017503
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017503.1, NP_001017503.1
• RefSeq Size: 2473 bp
• RefSeq ORF: 948 bp
• Locus ID: 498407
• Cytogenetics: 14q21
• MW: 33.4 kDa