Hexa (NM_001004443) Rat Tagged ORF Clone

CAT#: RR204407

Hexa (Myc-DDK-tagged ORF) - Rat hexosaminidase A (Hexa), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hexa
• Synonyms: MGC95289
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204407 representing NM_001004443
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCTGCAGGCTCTGGGTTTCGCTGCTGCTGGCGGCGGCGTTGGCTTGCTTGGCCACGGCGCTGT
  GGCCCTGGCCCCAGTACATCCAAACCTCCCACCGGCGCTACACCCTGTACCCCAACAACTTCCAGTTCCG
  GTACCATGCCGGTTCGGCCGCGCAGGCGGGCTGCGTTGTCCTCGACGAGGCCTTTCGACGCTACCGTAGC
  CTGCTCTTCGGTTCCGGCTCTTGGCCCCGACCCAGCTTCTCAAAAAAACAGCAGCCGTTGGGGAAGAACA
  TTCTGATGGTCTCTGTCGTCACAGCCGAATGTAATGAGTTTCCTAATTTGGAGTCCGTAGAAAATTACAC
  CCTAACCATTAATGATGACCAGTGTTTACTCTCCTCTGAGACTGTCTGGGGCGCTCTGCGAGGTCTGGAG
  ACTTTCAGTCAGCTTGTTTGGAAGTCAGCTGAGGGCACGTTCTTTATCAACAAGACAAAGATTACAGACT
  TTCCTCGATTCCCTCACCGGGGCATACTGCTGGATACATCTCGCCATTACCTGCCGTTATCTAGCATCCT
  GAACACACTGGATGTCATGGCGTACAATAAATTCAACGTGTTCCACTGGCACTTGGTGGACGACTCTTCC
  TTCCCATACGAGAGCTTCACCTTCCCAGAGCTCACCAGAAAGGGGTCCTTCAACCCTGTTACCCACATCT
  ACACAGCACAGGATGTGAAGGAGGTCATTGAATATGCAAGGCTTCGGGGTATCCGAGTGCTGGCCGAATT
  TGACACTCCTGGCCACACTTTGTCCTGGGGGGCAGGTGTCCCTGGATTATTAACACCTTGCTACTCTGGG
  TCCCGTCTCTCTGGCACCTATGGACCTGTGAACCCCAGCCTCAACAGCACCTACGACTTCATGAGCACAT
  TCTTCCTGGAGATCAGCTCTGTCTTCCCTGACTTTTATCTCCACCTGGGAGGAGATGAAGTTGACTTCAC
  CTGCTGGAAGTCCAACCCCAACATCCAGGCCTTCATGAAGAAAAAGGGCTTTACTGACTACAAGCAGCTG
  GAGTCATTCTACATCCAGACGCTGCTGGACATCGTCTCTGATTATGACAAGGGCTATGTGGTGTGGCAGG
  AAGTATTTGATAATAAAGTGAAGGTTCGGCCAGACACAATCATACAGGTGTGGCGGGAAGAGATGCCGGT
  CCAGTACATGAAGGAGATAGAGGCCATCACCCAGGCTGGCTTCCGGGCCCTGCTGTCTGCTCCCTGGTAC
  CTGAACCGGGTGAAGTATGGCCCTGACTGGAAGGAAATGTACAAAGTGGAGCCCCTGGCATTTCGTGGTA
  CACCTGCACAAAAGGCTCTGGTCATTGGAGGGGAAGCCTGTATGTGGGGAGAGTATGTGGACAGCACAAA
  CCTTGTCCCCAGGCTCTGGCCCAGAGCAGGTGCCATTGCCGAAAGACTGTGGAGCAGTAACCTGACGACT
  AATATGGACTTTGCCTTTAAACGTCTGTCCCATTTCCGCTGTGAGCTGCTGAGGAGAGGAATCCAGGCCC
  AGCCCATCAGCGTGGGCTACTGTGAGCAGGAGTTTGAGCATACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204407 representing NM_001004443
  Red=Cloning site Green=Tags(s)
  
  MAGCRLWVSLLLAAALACLATALWPWPQYIQTSHRRYTLYPNNFQFRYHAGSAAQAGCVVLDEAFRRYRS
  LLFGSGSWPRPSFSKKQQPLGKNILMVSVVTAECNEFPNLESVENYTLTINDDQCLLSSETVWGALRGLE
  TFSQLVWKSAEGTFFINKTKITDFPRFPHRGILLDTSRHYLPLSSILNTLDVMAYNKFNVFHWHLVDDSS
  FPYESFTFPELTRKGSFNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGAGVPGLLTPCYSG
  SRLSGTYGPVNPSLNSTYDFMSTFFLEISSVFPDFYLHLGGDEVDFTCWKSNPNIQAFMKKKGFTDYKQL
  ESFYIQTLLDIVSDYDKGYVVWQEVFDNKVKVRPDTIIQVWREEMPVQYMKEIEAITQAGFRALLSAPWY
  LNRVKYGPDWKEMYKVEPLAFRGTPAQKALVIGGEACMWGEYVDSTNLVPRLWPRAGAIAERLWSSNLTT
  NMDFAFKRLSHFRCELLRRGIQAQPISVGYCEQEFEHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004443
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004443.1, NP_001004443.1
• RefSeq Size: 1779 bp
• RefSeq ORF: 1587 bp
• Locus ID: 300757
• Cytogenetics: 8q24
• MW: 60.5 kDa
• Gene Summary: mouse homolog is the alpha subunit of lysosomal beta-hexosaminidase A [RGD, Feb 2006]