Mef2a (NM_001014035) Rat Tagged ORF Clone

CAT#: RR204477

Mef2a (Myc-DDK-tagged ORF) - Rat myocyte enhancer factor 2a (Mef2a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mef2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204477 representing NM_001014035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGAAAGAAAATACAAATTACACGCATAATGGATGAGAGGAACCGACAGGTGACTTTTACAAAGC
  GAAAGTTCGGATTGATGAAGAAAGCCTATGAACTCAGTGTGCTCTGTGACTGTGAGATAGCACTCATCAT
  CTTCAACAGCTCTAACAAGTTGTTTCAGTACGCCAGCACTGACATGGACAAAGTCCTTCTCAAGTACACC
  GAGTACAACGAGCCTCATGAAAGCAGGACGAACTCGGATATTGTGGAGGCTCTGAACAAGAAGGAACACA
  GAGGCTGCGACAGCCCAGACCCTGATACTTCATATGTGCTAACTCCACATACAGAAGAAAAATATAAAAA
  AATTAATGAGGAATTTGATAATATGATGCGGAATCATAAAATCGCACCTGGCTTGCCGCCTCAGAACTTC
  TCAATGTCTGTCACAGTCCCTGTGACCAGCCCTAATGCTTTGTCGTACACTAACCCAGGGAGTTCACTCG
  TGTCACCGTCTTTGGCAGCCAGCTCAACATTAGCAGAGTCCAGCATGCTGTCTCCACCCCCAGCCACGCT
  ACATAGAAATGTGTCTCCAGGAGCTCCGCAGAGACCACCAAGCACTGGCAGTGCAGGTGGGATGTTGAGC
  ACTACAGACCTCACGGTACCAAATGGAGCTGGAAATAGTCCTGTGGGGAATGGATTTGTAAACTCAAGGG
  CCTCTCCAAATTTGATTGGAAATACTGGTGCGAATAGTGTAGGCAAAGTCATGCCTACAAAGTCCCCCCC
  GCCACCAGGTGGGGGCAGTGTTGGAATGAACAGTCGGAAACCAGATCTACGGGTTGTCATTCCCCCTTCA
  AGCAAGGGCATGATGCCCCCACTTAATGCCCAAAGGATAAGTAGTTCTCAAGCCACACAACCTCTTGCCA
  CGCCCGTGGTGTCTGTGACAACTCCAAGCTTGCCTCCGCAGGGACTAGTGTACTCGGCCATGCCGACAGC
  CTACAACACTGATTACTCATTGACCAGCGCTGACCTGTCCGCCCTGCAAGGCTTTACTTCCCCTGGAATG
  CTGTCTCTGGGGCAGGCTTCAGCCTGGCAGCAGCACCATCTAGGACAAGCGGCCCTCAGCTCTCTAGTGG
  CTGGAGGGCAGTTATCTCAGGGTTCAAATTTGTCCATTAACACCAACCAGAACATCAACATTAAGTCAGA
  GCCAATTTCACCTCCCCGTGATAGAATGACCCCATCAGGCTTCCAGCAGCAGCAGCAGCAGCAGCCACAG
  CAGCAACCACCGCCACAACCTCCACAGCCCCAGCCCCAACCCCGGCAGGAAATGGGGCGCTCCCCTGTGG
  ACAGTCTGAGCAGCTCTAGCAGCTCCTACGATGGCAGTGACCGGGAGGATCCACGGGGCGACTTCCATTC
  TCCAATTGTGCTTGGCCGACCTCCAAATGCTGAGGACAGAGAAAGCCCCTCTGTAAAACGCATGAGGATG
  GACACATGGGTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204477 representing NM_001014035
  Red=Cloning site Green=Tags(s)
  
  MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYT
  EYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHTEEKYKKINEEFDNMMRNHKIAPGLPPQNF
  SMSVTVPVTSPNALSYTNPGSSLVSPSLAASSTLAESSMLSPPPATLHRNVSPGAPQRPPSTGSAGGMLS
  TTDLTVPNGAGNSPVGNGFVNSRASPNLIGNTGANSVGKVMPTKSPPPPGGGSVGMNSRKPDLRVVIPPS
  SKGMMPPLNAQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTSADLSALQGFTSPGM
  LSLGQASAWQQHHLGQAALSSLVAGGQLSQGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQQQQQPQ
  QQPPPQPPQPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNAEDRESPSVKRMRM
  DTWVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014035
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014035.1, NP_001014057.1
• RefSeq Size: 3237 bp
• RefSeq ORF: 1488 bp
• Locus ID: 309957
• Cytogenetics: 1q22
• MW: 53.3 kDa