Adh6 (NM_001012084) Rat Tagged ORF Clone

CAT#: RR204593

Adh6 (Myc-DDK-tagged ORF) - Rat alcohol dehydrogenase 6 (class V) (Adh6), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adh6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204593 representing NM_001012084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCCAGGGAAAGGTGATCAGATGCAAAGCCACTGTGCTCTGGAAGCCTGGAGCCCCACTTGCCA
  TTGAGGAGATCGAAGTGGCCCCACCTAAAGCAAAGGAGGTTCGAATAAAGATGGTGGCCACAGGAGTCTG
  TGGCACAGACATAAAACATCTGGACACACAAGAACTTTCTAAGTTTTGCCCCATGATTATGGGCCATGAA
  GGAGTTGGGATAGTCGAGAGTGTGGGGGAAGGAGTAAGCAGCGTAAGAACAGGTGATAAAGTTATCCTAT
  TATGTATCCCTCAGTGTGGGGAATGCAAAACTTGCCTGAATTCCAAGAACAATATTTGTACAGAAATTAG
  ACTGTCAAAGACACACCTGGCATCTGAAGGCACCAGCAGAATTACCTGCAAGGGAAAGTTAGTGCATCAG
  TATATCGCTCTCGGCAGCTTCTCGGAATACACAGTCCTAAAGGAAATCTCAGTCGCCAAGATTGACGAAG
  GTGCACCTCTGGAGAAGGTGTGCATAATTGGCTGTGGGTTTGCCACTGGGTACGGCGCTGCCATCAACTC
  TGCCAAGGTGACCCCAGGCTCCACCTGTGCGGTGTTTGGCCTCGGAGGTGTCGGTCTGTCTGTCATCATT
  GGCTGTAAAGCAGCAGGAGCAGCCAGGATCATTGCCGTGGACATCAACAAAGACAGGTTTGCGAAGGCCA
  AGACAGTGGGTGCCACTGACTGCGTCGACCCACGGGACTTCGAGAAACCCATTGAGGAAGTTTTATCCGA
  TATGATAGATGGTGGCGTGGACTTCTGCTTTGAGGTCACTGGGAACACAGAAGCAGTGGGAGCTGCCCTG
  GGCTCCTGTCACAAGGACCACGGGGTCTGCGTGACTGTTGGAGCCCTAGCATCTTTCACCAGTACACTCA
  GCATTAGAAGCCACTTGTTCTTCTCAGGACGCATTCTGAAAGGGTCTATTTTGGGAGGCTGGAAAACGAA
  AGAAGAAATCCCTAAGCTGGTTTCTGACTATATGGCAAAGAAGTTTAACATAGATCCTCTGATCACCCAC
  ACCCTGACTCTCAGCGAGGCCAATGAGGCAGTTCAGCTCATGAAAAGTGGGCAATGTATCCGCTGTGTCC
  TGCTCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204593 representing NM_001012084
  Red=Cloning site Green=Tags(s)
  
  MGTQGKVIRCKATVLWKPGAPLAIEEIEVAPPKAKEVRIKMVATGVCGTDIKHLDTQELSKFCPMIMGHE
  GVGIVESVGEGVSSVRTGDKVILLCIPQCGECKTCLNSKNNICTEIRLSKTHLASEGTSRITCKGKLVHQ
  YIALGSFSEYTVLKEISVAKIDEGAPLEKVCIIGCGFATGYGAAINSAKVTPGSTCAVFGLGGVGLSVII
  GCKAAGAARIIAVDINKDRFAKAKTVGATDCVDPRDFEKPIEEVLSDMIDGGVDFCFEVTGNTEAVGAAL
  GSCHKDHGVCVTVGALASFTSTLSIRSHLFFSGRILKGSILGGWKTKEEIPKLVSDYMAKKFNIDPLITH
  TLTLSEANEAVQLMKSGQCIRCVLLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012084
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012084.1, NP_001012084.1
• RefSeq Size: 1854 bp
• RefSeq ORF: 1131 bp
• Locus ID: 310903
• Cytogenetics: 2q44
• MW: 39.7 kDa