Cacna1a (NM_012918) Rat Tagged ORF Clone

CAT#: RR204610

Cacna1a (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (Cacna1a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1a
• Synonyms: BccA1; Cav2.1; rbA-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204610 representing NM_012918
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCTTTGGAGACGAGATGCCGGGCCGCTACGGCGCAGGCGGAGGAGGCTCAGGGCCGGCCGCCG
  GGGTGGTCGTGGGCGCCGCGGGCGGCCGAGGAGCCGGGGGCAGCCGGCAGGGCGGGCAGCCCGGAGCGCA
  GAGGATGTACAAGCAGTCGATGGCGCAGAGAGCGCGGACCATGGCCCTCTACAACCCCATCCCTGTCCGC
  CAGAACTGCCTCACGGTCAACCGCTCCCTGTTCCTCTTCAGTGAAGACAACGTGGTGAGAAAATACGCCA
  AAAAGATCACGGAATGGCCTCCCTTCGAGTACATGATCCTGGCCACCATCATTGCTAACTGCATCGTCCT
  GGCCCTGGAGCAGCACCTCCCTGATGATGACAAGACACCCATGTCCGAGCGGCTGGATGACACAGAACCC
  TATTTCATTGGCATCTTCTGTTTTGAGGCTGGAATTAAGATCGTGGCTCTTGGCTTTGCCTTCCACAAAG
  GCTCCTACCTGAGGAATGGCTGGAACGTCATGGACTTTGTCGTGGTGCTAACAGGCATCTTGGCCACTGT
  CGGGACGGAGTTTGATCTACGGACACTGAGGGCGGTTCGTGTGCTGCGGCCACTCAAGCTGGTGTCTGGA
  ATCCCAAGTTTACAAGTCGTCCTGAAGTCAATCATGAAGGCGATGATCCCTCTGCTGCAGATCGGCCTCC
  TCCTGTTTTTTGCAATCCTTATTTTTGCAATCATAGGGTTAGAATTTTATATGGGAAAATTTCATACCAC
  CTGCTTTGAAGAGGGGACAGACGACATCCAGGGTGAGTCGCCAGCTCCGTGTGGGACAGAGGAGCCTGCC
  CGCACCTGCCCCAACGGGACCAAATGTCAGCCGTACTGGGAAGGGCCCAACAACGGCATCACTCAGTTCG
  ACAACATCCTGTTTGCTGTGCTCACTGTTTTCCAGTGCATCACCATGGAAGGCTGGACTGATCTCCTCTA
  CAATAGCAACGATGCCTCAGGGAACACTTGGAACTGGTTGTACTTCATCCCCCTCATCATCATCGGCTCC
  TTTTTTATGCTGAACCTTGTGCTGGGTGTGCTGTCTGGGGAGTTTGCCAAAGAAAGGGAACGTGTAGAGA
  ACCGAAGGGCTTTTCTGAAGCTCAGAAGACAACAGCAGATTGAACGTGAGCTCAATGGATACATGGAGTG
  GATCTCGAAAGCAGAAGAGGTGATCCTCGCGGAGGACGAGACAGACGTGGAGCAGAGGCACCCTTTTGAT
  GGAGCTCTTCGGAGAGCTACTCTGAAGAAAAGCAAGACGGACCTGCTCAACCCTGAGGAGGCGGAGGACC
  AGCTTGCTGACATCGCGTCTGTGGGGTCTCCCTTCGCCAGAGCCAGCATCAAAAGTGCCAAGCTGGAGAA
  TTCGACTTTTTTCCACAAAAAGGAGAGAAGAATGCGTTTCTACATCCGCCGCATGGTCAAAACTCAGGCC
  TTCTACTGGACCGTGCTCAGTCTGGTAGCCCTCAACACGCTGTGGCTCGCCATTGTCCACTACAACCAGC
  CCGAGTGGCTCTCCGACTTCCTCTACTATGCAGAATTCATTTTCTTAGGACTCTTTATGTCCGAAATGTT
  TATAAAAATGTATGGGCTCGGGACACGGCCTTACTTCCACTCTTCCTTCAACTGCTTTGACTGTGGGGTC
  ATCATCGGGAGCATCTTTGAAGTCATCTGGGCCGTCATCAAACCGGGTACATCCTTTGGAATCAGCGTGT
  TACGAGCTCTCAGGTTACTGCGTATTTTCAAAGTCACAAAGTACTGGGCATCTCTCAGAAACCTGGTTGT
  CTCCCTCCTCAACTCCATGAAATCCATCATAAGTCTGCTGTTCCTCCTCTTCCTCTTCATTGTCGTCTTT
  GCCCTCTTGGGGATGCAGCTGTTTGGTGGCCAGTTTAATTTTGACGAGGGGACTCCTCCCACCAACTTCG
  ACACTTTTCCAGCAGCAATAATGACTGTGTTTCAGATCCTGACTGGCGAGGATTGGAATGAGGTCATGTA
  TGATGAGATCAAGTCTCAGGGGGGCGTGCAGGGCGGCATGGTGTTCTCCATCTACTTCATCGTCCTCACC
  CTCTTCGGGAACTACACCCTGCTGAACGTGTTCTTAGCTATCGCGGTGGACAACCTGGCCAACGCCCAGG
  AACTCACCAAGGATGAACAAGAAGAGGAAGAGGCAGCCAATCAGAAACTGGCTCTACAGAAAGCCAAGGA
  GGTGGCAGAAGTGAGTCCCCTGTCTGCAGCCAACATGTCCATAGCTGTGAAGGAACAGCAGAAGAACCAG
  AAGCCTGCCAAGTCGGTGTGGGAGCAGCGCACCAGCGAGATGCGCAAGCAGAACCTGCTGGCTAGCCGCG
  AGGCGCTGTACGGGGACGCGGCTGAGCGCTGGCCCACCACTTACGCGCGCCCGCTGCGGCCGGACGTGAA
  GACGCACTTGGACCGGCCGCTCGTGGTGGACCCGCAGGAGAACCGTAACAACAACACCAACAAGAGCCGT
  GCGCCAGAAGCGCTGCGCCAAACCGCGCGGCCCCGCGAGAGCGCGCGCGACCCCGACGCGCGGCGCGCCT
  GGCCCAGCAGCCCTGAGCGCGCCCCTGGACGAGAGGGCCCGTATGGCCGCGAGAGCGAGCCGCAACAGCG
  CGAGCACGCGCCACCCCGCGAGCACGTACCCTGGGACGCGGATCCTGAGCGCGCCAAGGCCGGGGACGCG
  CCCCGCCGCCACACGCACCGGCCTGTGGCCGAGGGCGAGCCTCGTCGCCACCGCGCGCGCCGCCGGCCCG
  GGGACGAACCGGACGACAGACCGGAGCGCAGGCCGCGTCCCCGCGACGCCACTAGGCCGGCCCGCGCTGC
  AGACGGCGAAGGCGATGATGGGGAGCGCAAGCGGCGACACCGACACGGGCCGCCGGCCCACGATGACAGG
  GAGCGCAGACACCGGCGCAGAAAAGAGAGCCAGGGCTCTGGGGTCCCCATGTCTGGTCCCAACCTGTCCA
  CCACCAGGCCAATCCAGCAGGATCTGGGCCGCCAGGACCTGCCACTGGCTGAGGACCTGGACAACATGAA
  GAACAACAAGTTGGCCACCGGGGAGCCTGCCAGTCCCCACGACAGCCTGGGCCACAGTGGCCTTCCCCCT
  AGCCCTGCCAAGATCGGGAACAGCACCAACCCTGGTCCCGCCTTGGCCACCAATCCCCAGAATGCTGCCA
  GCCGCAGGACGCCCAACAACCCGGGCAACCCGTCCAACCCCGGCCCCCCCAAGACTCCCGAGAACAGCCT
  TATCGTCACCAACCCCAGCAGCACCCAGCCCAACTCAGCAAAGACTGCCAGGAAACCCGAGCACATGGCG
  GTGGAGATCCCCCCGGCCTGCCCGCCCCTCAACCACACTGTGGTCCAAGTAAACAAAAACGCCAACCCAG
  ACCCACTGCCAAAGAAAGAGGAAGAGAAGAAGGAGGAAGAGGAGGCAGACCCCGGGGAGGATGGCCCAAA
  GCCCATGCCGCCCTACAGCTCCATGTTCATCCTCTCCACCACCAACCCCCTTCGCCGGCTGTGCCATTAC
  ATCCTGAACCTGCGCTACTTCGAGATGTGCATCCTCATGGTCATTGCCATGAGTAGCATCGCGCTGGCCG
  CCGAGGACCCGGTGCAGCCCAACGCACCCCGCAACAACGTGCTGCGATATTTTGACTATGTTTTCACAGG
  AGTGTTTACCTTTGAGATGGTGATCAAGATGATCGACCTGGGCCTCGTCCTGCATCAGGGGGCCTATTTC
  CGTGACCTGTGGAACATTCTGGACTTCATAGTGGTCAGTGGGGCCCTGGTGGCCTTTGCCTTCACTGGCA
  ATAGCAAAGGAAAGGACATCAACACCATCAAGTCCCTCCGAGTCCTCCGGGTGCTACGACCTCTAAAGAC
  CATCAAGCGGCTGCCTAAGTTGAAGGCCGTATTTGACTGCGTGGTGAACTCGCTCAAGAACGTCTTCAAC
  ATCCTCATTGTCTACATGCTCTTCATGTTCATCTTCGCCGTGGTGGCCGTGCAGCTCTTCAAGGGCAAAT
  TCTTCCACTGCACGGACGAGTCCAAGGAGTTTGAGAGAGACTGTCGAGGCAAATACCTCCTTTACGAGAA
  GAACGAGGTAAAGGCGCGGGACCGCGAGTGGAAGAAATACGACTTCCACTACGACAACGTGCTCTGGGCC
  CTGCTCACGCTCTTTACGGTGTCCACGGGAGAGGGCTGGCCACAGGTCCTCAAGCACTCAGTGGATGCCA
  CTTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAAATGTCCATCTTCTACGTGGTCTACTTTGT
  GGTGTTTCCCTTCTTCTTTGTCAATATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAGGGAGAC
  AAGATGATGGAAGAATACAGCCTAGAGAAAAATGAGAGGGCCTGCATCGACTTTGCCATCAGTGCCAAGC
  CGCTGACCAGGCACATGCCCCAGAACAAGCAGAGCTTCCAGTATCGAATGTGGCAGTTCGTGGTGTCCCC
  ACCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAGTTCTATGGA
  GCCTCTGTGGCCTATGAAAACGCCCTTCGAGTGTTCAACATTGTCTTCACCTCCCTCTTCTCTCTCGAAT
  GTGTGCTCAAAGTCATGGCTTTTGGGATTCTGAATTATTTCCGCGATGCCTGGAACATCTTCGACTTTGT
  GACTGTTCTGGGCAGCATCACAGACATCCTCGTCACCGAGTTTGGGAATAACTTCATCAACCTGAGCTTT
  CTCCGCCTCTTCCGTGCTGCCCGACTCATCAAACTCCTCCGCCAGGGTTACACCATCCGCATTCTCCTCT
  GGACTTTCGTGCAGTCTTTCAAGGCCCTACCTTATGTCTGTCTGCTGATCGCCATGCTCTTCTTCATCTA
  TGCCATCATCGGGATGCAGGTGTTTGGCAACATCGGCATTGATGGGGAAGATGAGGACAGCGATGAGGAT
  GAGTTCCAAATCACGGAGCACAATAACTTCCGGACCTTCTTCCAAGCTCTCATGCTTCTCTTCCGGAGCG
  CCACAGGGGAAGCGTGGCACAACATCATGCTGTCCTGCCTCAGCGGGAAGCCATGCGACAAGAACTCCGG
  GATCCAAAAACCAGAGTGTGGCAACGAGTTCGCCTATTTTTACTTTGTCTCGTTCATCTTCCTTTGCTCA
  TTTCTGATGCTGAATCTCTTTGTTGCTGTCATCATGGACAACTTCGAGTACCTCACCCGAGATTCCTCCA
  TCCTGGGCCCCCACCACCTGGATGAGTACGTGCGTGTCTGGGCAGAGTATGACCCTGCTGCCTGCGGCCG
  CATTCACTATAAGGACATGTACAGTTTATTGCGAGTAATATCGCCCCCTCTCGGCTTAGGCAAGAAATGT
  CCTCATAGGGTTGCTTGCAAGAGGCTCTTGCGGATGGACCTACCCGTAGCGGATGACAACACCGTTCACT
  TCAACTCCACCTTGATGGCTCTGATCCGAACCGCCCTGGATATCAAAATCGCAAAGGGTGGAGCTGACAA
  GCAGCAAATGGACGCAGAGCTCCGCAAGGAAATGATGGCCATTTGGCCCAACCTGTCTCAGAAGACCTTG
  GATCTGCTGGTCACACCTCACAAGTCCACGGACCTGACAGTGGGTAAGATCTACGCAGCCATGATGATCA
  TGGAGTACTACCGGCAGAGCAAGGCCAAGAAGCTGCAGGCCATGCGAGAGGAGCAGAACCGGACACCACT
  CATGTTCCAGCGCATGGAGCCTCCATCGCCAACACAGGAGGGAGGACCCAGCCAAAACGCCCTTCCCTCC
  ACTCAGCTGGACCCCGGAGGAGGCCTGATGGCTCAAGAAAGCAGCATGAAGGAGAGCCCGTCCTGGGTGA
  CCCAGCGGGCACAGGAGATGTTCCAGAAGACTGGTACCTGGAGCCCAGAGCGAGGGCCACCCATCGACAT
  GCCTAACAGCCAGCCCAACTCCCAGTCTGTGGAGATGAGAGAAATGGGAACTGATGGCTACTCAGACAGC
  GAACACTACCTCCCCATGGAAGGACAGACCAGGGCCGCCTCCATGCCCCGCCTCCCAGCAGAGAACCAGA
  GGAGAAGGGGCCGGCCACGTGGAAATAACCTCAGTACCATCTCTGATACCAGCCCCATGAAGCGCTCAGC
  CTCCGTGCTGGGACCCAAAGCCCGGCGACTGGATGACTACTCACTAGAGCGGGTACCACCTGAGGAGAAC
  CAAAGGTACCACCAACGCCGCCGGGACCGTGGCCACCGCACCTCTGAGCGCTCTCTGGGCCGATACACTG
  ATGTGGACACAGGCCTGGGGACAGATCTGAGCATGACCACCCAATCGGGTGACCTGCCCTCCAAAGATCG
  GGACCAGGACCGGGGCCGGCCCAAGGACCGGAAGCATCGGCCACACCACCACCACCACCATCATCACCAT
  CATCCCCCGGCCCCGGACCGGGAGCGCTACGCACAGGAGCGGCCGGACACCGGCCGGGCGCGGGCCCGGG
  AGCAGCGCTGGTCCCGCTCGCCCAGCGAGGGTCGGGAGCACGCGACACACAGACAGGGCAGTAGTTCCGT
  CAGTGGAAGCCCAGCCCCCTCTACGTCCGGCACCAGCACGCCGCGGCGCGGCCGCCGCCAGCTCCCCCAG
  ACCCCCTGCACCCCGCGGCCGCTTGTGTCCTACTCGCCCGCTCCGCGCAGGCCTGCGGCGCGCAGGATGG
  CGGGTCCCCCAGCGCCCCCTGGCGGCTCGCCGCGGGGTTGCCGCCGTGCGCCGCGCTGGCCTGCGCATGC
  GCCTGAGGGCCCGCGGCCGCGCGGTGCCGACTACACCGAGCCCGACAGCCCGCGCGAGCCGCCAGGGGGC
  GCGCATGAGCCCGCGCCGCGGTCGCCCAGGACTCCGCGCGCCGCGGGCTGCGCGTCACCGCGCCACGGCC
  GCAGGCTGCCCAATGGTTACTACGCGGGGCACGGCGCGCCACGGCCGCGCACAGCCCGCAGGGGCGCGCA
  CGACGCGTACAGCGAGAGCGAGGACGACTGGTGC
  
  CTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
  ATAAGGTTTAA
  Protein Sequence

  >RR204610 representing NM_012918
  Red=Cloning site Green=Tags(s)
  
  MARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVR
  QNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEP
  YFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSG
  IPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPA
  RTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGS
  FFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFD
  GALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQA
  FYWTVLSLVALNTLWLAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGV
  IIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVF
  ALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDEIKSQGGVQGGMVFSIYFIVLT
  LFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQ
  KPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSR
  APEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDA
  PRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDR
  ERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPP
  SPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMA
  VEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHY
  ILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYF
  RDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFN
  ILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWA
  LLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGD
  KMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYG
  ASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSF
  LRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDED
  EFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCS
  FLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKC
  PHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTL
  DLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPS
  TQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDS
  EHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEEN
  QRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHH
  HPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQ
  TPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGG
  AHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC
  
  LEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012918
• ORF Size: 7104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012918.2, NM_012918.3, NP_037050.2
• RefSeq Size: 8912
• RefSeq ORF: 7107
• Locus ID: 25398
• MW: 268 kDa
• Gene Summary: voltage-gated calcium channel that may be involved in secretion coupling in pancreatic beta cells [RGD, Feb 2006]