Wnt5b (NM_001100489) Rat Tagged ORF Clone

CAT#: RR204691

Wnt5b (Myc-DDK-tagged ORF) - Rat wingless-type MMTV integration site family, member 5B (Wnt5b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt5b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204691 representing NM_001100489
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGTCCCTGGGCACTGGGATGGGTTGAGGCCGGCCATGCCCAGCCTGCTGCTGCTGTTCGTGGCAG
  CTCTGCTCTCCAGCTGGGCACAGCTGCTGACTGACGCCAACTCCTGGTGGTCGCTGGCTCTGAACCCGGT
  GCAGAGACCCGAGATGTTCATCATTGGCGCTCAGCCCGTGTGCAGCCAACTTCCCGGGCTTTCGCCAGGC
  CAGCGAAAGCTGTGTCAGTTGTATCAGGAGCACATGTCCTACATCGGGGAGGGAGCCAAGACGGGCATCA
  GGGAGTGCCAGCACCAGTTTCGACAGAGGCGGTGGAACTGCAGCACCGTGGACAACTTGTCTGTCTTTGG
  CAGGGTCATGCAGATAGGTAGCCGAGAGACTGCCTTCACATATGCGGTGAGCGCTGCTGGTGTGGTGAAT
  GCCATCAGCCGAGCCTGCAGAGAGGGTGAGCTGTCCACCTGTGGCTGCAGCCGTGCAGCGAGGCCCAAGG
  ACCTGCCACGAGACTGGTTGTGGGGCGGCTGTGGGGACAACGTGGAGTATGGCTACCGCTTTGCCAAGGA
  GTTTGTGGATGCCCGAGAGCGTGAGAAGAACTTTGCCAAGGGATCAGAGGAGCAGGGCCGAGCCCTCATG
  AACTTACAGAACAACGAGGCTGGCCGCCGGGCCGTGTATAAGATGGCTGATGTCTCCTGCAAATGTCACG
  GAGTCTCCGGGTCCTGCAGCCTCAAGACCTGCTGGCTCCAGCTGGCTGAGTTCCGCAAGGTGGGGGACCG
  TTTGAAAGAGAAGTATGACAGCGCTGCGGCCATGCGTATCACCCGTCAGGGCAAGCTGGAACTGACCAAC
  AGCCGCTTCAACCAGCCCAGCCCGGAGGACCTGGTCTACGTAGACCCCAGCCCTGACTACTGCCTGCGAA
  ATGAGACCACGGGCTCCCTGGGCACCCAAGGTCGCCTCTGCAACAAGACCTCAGAGGGCACGGATGGCTG
  CGAGCTCATGTGCTGTGGCCGCGGCTATGACCGCTTCAAGAGCGTCCAGGTGGAACGCTGCCACTGCAGG
  TTCCACTGGTGTTGCTTTGTCAGATGCAAAAAATGCACCGAGATCGTGGACCAGTATGTCTGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204691 representing NM_001100489
  Red=Cloning site Green=Tags(s)
  
  MLVPGHWDGLRPAMPSLLLLFVAALLSSWAQLLTDANSWWSLALNPVQRPEMFIIGAQPVCSQLPGLSPG
  QRKLCQLYQEHMSYIGEGAKTGIRECQHQFRQRRWNCSTVDNLSVFGRVMQIGSRETAFTYAVSAAGVVN
  AISRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNVEYGYRFAKEFVDAREREKNFAKGSEEQGRALM
  NLQNNEAGRRAVYKMADVSCKCHGVSGSCSLKTCWLQLAEFRKVGDRLKEKYDSAAAMRITRQGKLELTN
  SRFNQPSPEDLVYVDPSPDYCLRNETTGSLGTQGRLCNKTSEGTDGCELMCCGRGYDRFKSVQVERCHCR
  FHWCCFVRCKKCTEIVDQYVCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100489
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001100489.1, NP_001093959.1
• RefSeq Size: 2122
• RefSeq ORF: 1119
• Locus ID: 282582
• MW: 41.9 kDa
• Gene Summary: mouse homolog regulates chondrocyte differentiation and proliferation in skeletal development [RGD, Feb 2006]