Lonp1 (NM_133404) Rat Tagged ORF Clone

CAT#: RR204779

Lonp1 (Myc-DDK-tagged ORF) - Rat lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lonp1
• Synonyms: Lon; Prss15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204779 representing NM_133404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAAGCACAGGTTACGTGCGGCTGTGGGCTGCTGCGCGGTGCTGGGTTCTGCGGCGTCCGCTCT
  TGGCTGTCACCGGAGGCCGAGTCCCGAGTGCGTCCGGGTCCTGGCTGCGCCGAGGATGTCGGGTCTGCGA
  CACGTCAACTCCCTGGGGCGGCCGCGTTCCTATGGGCGGGGGCCAGTGGCGGGGGCTGTGGGACGCGGGG
  AGCCGCGGTGGCAGCGACGAGACCTCCGAGGGCGGCGTGGAGGACGGGGCCACGGCGAGCAGCGGGGAAG
  GCCCGGTCGTCACGGCCCTCGCGCCTATGACCGTCCCGGATGTGTTTCCTCACCTGCCGCTCATCGCCAT
  CTCCCGCAACCCGGTGTTCCCGCGCTTTATCAAGATCGTGGAGGTTAAAAATAAGAAGTTGGTTGAGCTG
  CTGAGAAGGAAAGTCCGCCTGGCACAGCCGTATGTTGGCGTCTTCTTAAAGAGAGATGATAACAATGAGT
  CAGACGTGGTGGAGAGCCTGGATGAGATCTACCATACGGGAACGTTCGCTCAGATTCATGAGATGCAGGA
  CCTCGGAGACAAGTTGCGCATGATCGTTACAGGCCACAGAAGGATTCACATCAGCCGGCAGCTGGAGGTG
  GAACCAGAGGGGCTGGAGCCAGAGGCAGAGAACAAACAAAAGTCCAGGAGGAAGCTGAAGCGAGGCAAGA
  AAGAGGTCGGGGATGAGCTGGGTGCCAAGCCCCAGCTGGAGATGGTGACTGAGGCTACCAGTGACACATC
  CAAAGAGGTGCTCATGGTGGAGGTTGAGAATGTAGCCCATGAAGACTTTCAGGTCACAGAGGAAGTGAAG
  GCCCTGACTGCAGAGATCGTGAAGACCATTCGGGATATCATCGCCCTGAACCCTCTGTACAGAGAGTCCG
  TGCTGCAGATGATGCAGGCAGGCCAGCGTGTGGTGGACAACCCCATCTACTTGAGCGACATGGGTGCCGC
  ACTCACTGGGGCTGAGTCCCATGAGCTGCAAGATGTGCTGGAAGAAACAAACATCCTTAAGCGGCTCTAT
  AAGGCCCTGTCTCTTTTGAAGAAGGAGTTCGAGTTGAGCAAGCTTCAACAGCGCCTGGGGCGTGAGGTGG
  AGGAGAAGATCAAGCAGACGCACAGGAAGTACCTGCTGCAGGAGCAGCTAAAGATCATCAAGAAAGAGTT
  GGGACTAGAGAAGGATGATAAAGATGCCATTGAGGAGAAGTTCCGGGAACGCTTAAAGGAGCTTGTGGTT
  CCCAAGCATGTGATGGACGTGGTAGATGAGGAGCTGAGCAAGCTGGCCCTACTGGACAACCACTCCTCTG
  AATTCAATGTCACTCGCAACTACCTGGACTGGCTGACGTCCATCCCATGGGGCCGGCAGAGCGATGAGAA
  CTTAGACCTGGCTCGGGCCCAGTCGGTGCTGGAGGAGGACCACTATGGCATGGAGGATGTGAAGAAGCGA
  GTCTTGGAGTTCATTGCAGTCAGCCAGCTCCGAGGCTCCACACAGGGCAAGATCCTCTGCTTCCACGGCC
  CACCAGGTGTGGGCAAGACCAGCATTGCACGCTCCATTGCCCGTGCCCTTGGCCGTGAATACTTCCGCTT
  TAGTGTTGGTGGCATGACAGATGTGGCAGAAATCAAGGGGCACAGGCGTACCTATGTGGGGGCCATGCCT
  GGGAAGATTATCCAGTGTCTGAAGAAAACCAAGACAGAGAACCCATTAGTGCTGATTGATGAGGTGGATA
  AGATTGGCCGAGGCTACCAAGGGGACCCGTCATCAGCACTGTTGGAGTTGTTGGACCCTGAGCAGAATGC
  CAACTTCCTGGACCACTACCTGGATGTGCCAGTGGACCTGTCCAAGGTGCTATTCATCTGCACAGCCAAC
  GTCACTGACACTATACCGGAGCCACTAAGGGATCGCATGGAGATGATCAATGTGTCAGGCTATGTAGCAC
  AGGAGAAGCTGGCCATTGCAGAGCGGTACCTGGTGCCACAGGCCCGCACCCTGTGTGGCCTAGACGAGAG
  CAAGGCCCAACTGTCAGCGACAGTGCTCACTCTACTCATCAAACAGTACTGCAGGGAAAGTGGTGTGCGC
  AACCTGCAGAAGCAGGTGGAGAAGGTGCTGCGCAAGGCTGCATACAAGATTGTAAGTGGAGAGGCCCAGA
  CAGTGCACGTGACACCTGAGAACCTGCAAGACTTTGTAGGGAAGCCAGTGTTCACAGTGGAGCGCATGTA
  TGATGTCACGCCTCCTGGTGTGGTCATGGGTTTGGCCTGGACTGCCATGGGAGGCTCCACATTGTTCGTG
  GAGACATCTTTAAGGAGGCCCCAGCCTAGTGGCAGCAAGGAGGACAAGGATGGCAGCCTAGAGGTGACAG
  GTCAGCTAGGAGATGTCATGAAAGAGAGTGCCCGCATAGCCTACACCTTTGCCCGTGCCTTCCTGATGGA
  GCAGGACCCTGAAAATGACTTTCTGGTCACATCCCACATCCACCTGCATGTGCCTGAGGGCGCTACCCCC
  AAGGATGGCCCTAGTGCAGGTTGCACCATTGTCACTGCATTGCTGTCCCTAGCTCTGGGGCAGCCAGTGT
  TGCAGAACCTGGCCATGACTGGGGAAGTCTCCCTCACTGGTAAAGTGTTGCCTGTGGGTGGCATCAAGGA
  GAAGACCATTGCGGCTAAGCGTGCGGGAGTGACCTGCATCATTTTGCCTGCTGAGAACAGGAAGGACTTC
  TCAGACTTGGCTCCCTTCATCACAGAGGGCCTGGAGGTTCACTTCGTGGAACACTACCGCGACATCTTCC
  GAATTGCCTTCCCCCTGCGAGAGCACCAGGAAGCACTGGCTGTGGAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204779 representing NM_133404
  Red=Cloning site Green=Tags(s)
  
  MAASTGYVRLWAAARCWVLRRPLLAVTGGRVPSASGSWLRRGCRVCDTSTPWGGRVPMGGGQWRGLWDAG
  SRGGSDETSEGGVEDGATASSGEGPVVTALAPMTVPDVFPHLPLIAISRNPVFPRFIKIVEVKNKKLVEL
  LRRKVRLAQPYVGVFLKRDDNNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVTGHRRIHISRQLEV
  EPEGLEPEAENKQKSRRKLKRGKKEVGDELGAKPQLEMVTEATSDTSKEVLMVEVENVAHEDFQVTEEVK
  ALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNILKRLY
  KALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVV
  PKHVMDVVDEELSKLALLDNHSSEFNVTRNYLDWLTSIPWGRQSDENLDLARAQSVLEEDHYGMEDVKKR
  VLEFIAVSQLRGSTQGKILCFHGPPGVGKTSIARSIARALGREYFRFSVGGMTDVAEIKGHRRTYVGAMP
  GKIIQCLKKTKTENPLVLIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTAN
  VTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARTLCGLDESKAQLSATVLTLLIKQYCRESGVR
  NLQKQVEKVLRKAAYKIVSGEAQTVHVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFV
  ETSLRRPQPSGSKEDKDGSLEVTGQLGDVMKESARIAYTFARAFLMEQDPENDFLVTSHIHLHVPEGATP
  KDGPSAGCTIVTALLSLALGQPVLQNLAMTGEVSLTGKVLPVGGIKEKTIAAKRAGVTCIILPAENRKDF
  SDLAPFITEGLEVHFVEHYRDIFRIAFPLREHQEALAVER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133404
• ORF Size: 2850 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133404.1, NP_596895.1
• RefSeq Size: 2969
• RefSeq ORF: 2853
• Locus ID: 170916
• MW: 105.8 kDa
• Gene Summary: mitochondrial ATP-dependent protease that displays increased expression under conditions of brain ischemia, hypoxia, and ER stress [RGD, Feb 2006]