Actn1 (NM_031005) Rat Tagged ORF Clone

CAT#: RR204782

Actn1 (Myc-DDK-tagged ORF) - Rat actinin, alpha 1 (Actn1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204782 representing NM_031005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCATTATGATTCCCAGCAGACCAATGACTATATGCAGCCTGAAGAGGACTGGGACCGGGACCTGC
  TCCTGGATCCGGCCTGGGAGAAGCAGCAGAGAAAGACCTTCACGGCCTGGTGCAACTCCCACCTGCGCAA
  AGCAGGGACCCAGATTGAGAACATTGAAGAGGACTTCCGAGATGGCCTGAAGCTCATGCTGCTCCTGGAG
  GTCATCTCAGGTGAGCGTTTGGCCAAGCCAGAGAGAGGCAAGATGCGAGTGCACAAGATCTCCAACGTCA
  ACAAGGCCCTGGATTTCATAGCCAGCAAAGGGGTCAAGCTGGTGTCCATTGGAGCTGAAGAAATTGTGGA
  CGGGAATGTGAAGATGACCCTGGGCATGATCTGGACCATCATCCTTCGTTTTGCCATCCAAGACATCTCT
  GTGGAAGAGACATCAGCTAAAGAAGGGTTGCTCCTGTGGTGTCAGAGGAAGACAGCCCCATATAAAAATG
  TCAACATCCAAAACTTCCACATCAGTTGGAAGGATGGTCTTGGTTTCTGTGCCTTGATCCACCGGCACCG
  GCCCGAGTTGATTGACTATGGAAAGCTTCGAAAGGATGATCCACTCACAAACCTGAACACAGCCTTTGAT
  GTGGCAGAGAGATACCTGGACATTCCAAAGATGCTGGATGCAGAAGACATCGTCGGAACTGCCCGGCCAG
  ATGAGAAAGCCATTATGACCTATGTGTCTAGCTTCTACCATGCCTTCTCTGGAGCCCAGAAGGCGGAAAC
  AGCAGCCAATCGCATCTGTAAAGTGTTGGCCGTCAATCAAGAGAATGAACAGCTTATGGAAGACTATGAG
  AAGCTGGCCAGTGATCTGTTAGAGTGGATCCGCCGCACCATCCCATGGCTGGAGAATCGGGTGCCCGAGA
  ACACCATGCAGGCCATGCAGCAGAAGCTGGAGGACTTCCGAGACTACCGACGCCTGCACAAGCCGCCCAA
  GGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTCAACACACTGCAGACCAAGCTGCGGCTAAGCAACCGG
  CCTGCCTTCATGCCCTCCGAGGGCAGGATGGTCTCGGATATCAACAATGCCTGGGGCTGCCTGGAACAGG
  CAGAGAAGGGCTACGAGGAGTGGTTGCTGAATGAGATCCGGAGGCTAGAGAGGTTGGACCACCTGGCAGA
  GAAGTTCCGGCAGAAGGCCTCCATCCATGAAGCCTGGACAGACGGCAAAGAAGCCATGCTGCGGCAGAAG
  GATTATGAGACGGCCACCTTGTCCGAGATCAAGGCCCTACTCAAGAAGCACGAGGCCTTTGAGAGTGACC
  TGGCTGCCCACCAGGACCGCGTGGAGCAGATCGCGGCCATTGCCCAGGAGCTTAATGAGCTGGACTATTA
  TGACTCACCAAGTGTCAACGCTCGATGCCAAAAGATCTGTGACCAGTGGGACAATCTAGGGGCCCTGACT
  CAGAAGCGGAGGGAAGCTTTAGAGCGAACAGAGAAGCTGCTGGAGACCATCGACCAGCTATACTTGGAGT
  ACGCCAAGAGGGCTGCACCCTTCAACAACTGGATGGAGGGAGCCATGGAGGACCTTCAAGACACCTTCAT
  CGTACATACCATTGAGGAGATCCAGGGACTGACCACAGCCCATGAGCAGTTCAAGGCTACCCTCCCGGAT
  GCAGACAAGGAGCGCCTGGCCATCCTGGGCATCCACAATGAAGTGTCCAAGATTGTCCAGACCTATCATG
  TCAACATGGCAGGCACCAACCCCTATACAACCATCACACCTCAGGAGATCAATGGCAAATGGGACCATGT
  ACGGCAGCTGGTGCCCCGGAGGGACCAGGCTCTAACAGAGGAGCACTCGAGGCAGCAGCACAACGAGAGG
  TTACGCAAGCAGTTTGGGGCACAAGCCAATGTCATCGGACCCTGGATCCAGACCAAGATGGAGGAGATTG
  GGAGGATCTCCATTGAGATGCACGGTACCCTGGAGGACCAACTCAGCCACCTGCGGCAGTATGAAAAGAG
  CATCGTCAATTATAAGCCAAAGATTGACCAGCTGGAGGGCGACCACCAGCTCATCCAGGAAGCACTTATC
  TTCGACAATAAGCACACCAACTACACAATGGAGCACATCCGTGTGGGCTGGGAGCAGCTGCTCACCACCA
  TTGCCAGGACCATCAATGAAGTGGAGAACCAGATCTTGACCCGGGATGCCAAAGGCATCAGCCAGGAACA
  GATGAACGAATTCCGGGCCTCCTTCAACCACTTTGACCGGGATCACTCCGGCACGTTGGGTCCCGAAGAG
  TTCAAAGCCTGCCTCATCAGCTTGGGTTATGATATTGGCAACGACCCCCAGGGAGAGGCAGAATTTGCCC
  GGATCATGAGCATTGTAGACCCCAACCGCTTGGGGGTAGTGACATTCCAGGCCTTCATCGACTTCATGTC
  CCGAGAGACGGCTGACACAGATACAGCGGATCAGGTCATGGCTTCCTTCAAGATCCTGGCTGGAGATAAG
  AATTACATCACCGGGGATGAGCTGCGCCGCGAGCTGCCACCTGACCAAGCCGAGTACTGCATCGCGAGAA
  TGGCCCCCTACGCGGGCCCTGACTCCGTGCCGGGTGCTCTAGACTACATGTCCTTCTCCACGGCGCTGTA
  CGGCGAGAGTGACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204782 representing NM_031005
  Red=Cloning site Green=Tags(s)
  
  MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLE
  VISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDIS
  VEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFD
  VAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYE
  KLASDLLEWIRRTIPWLENRVPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNR
  PAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQK
  DYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALT
  QKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPD
  ADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHSRQQHNER
  LRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALI
  FDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEE
  FKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDK
  NYITGDELRRELPPDQAEYCIARMAPYAGPDSVPGALDYMSFSTALYGESDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031005
• ORF Size: 2676 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031005.1, NM_031005.2, NM_031005.3, NP_112267.1
• RefSeq Size: 2956
• RefSeq ORF: 2679
• Locus ID: 81634
• MW: 103 kDa