Smurf1 (NM_001109598) Rat Tagged ORF Clone

CAT#: RR204842

Smurf1 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 4930431E10 (RGD1309707), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smurf1
• Synonyms: RGD1309707
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204842 representing NM_001109598
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAACCCCGGGACCCGTAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTATTGTGTGCCAAGA
  ACCTTGCAAAGAAAGACTTCTTCAGACTCCCCGACCCCTTTGCCAAGATTGTTGTGGACGGCTCTGGGCA
  GTGCCACTCAACCGACACTGTGAAAAACACCCTGGACCCAAAGTGGAACCAGCACTATGACCTGTACGTT
  GGGAAAACCGACTCGATAACCATCAGTGTGTGGAACCACAAGAAGATCCACAAGAAGCAGGGGGCTGGCT
  TCCTGGGCTGCGTGCGGCTGCTCTCCAATGCCATCAGCAGATTGAAAGATACCGGCTACCAGCGTTTGGA
  TCTATGCAAACTAAATCCCTCAGATACTGATGCAGTTCGTGGCCAAATAGTGGTCAGTTTACAGACCCGA
  GACAGAATAGGTGGTGGAGGGTCGGTGGTGGACTGCAGGGGACTGCTGGAGAACGAAGGAACGGTATATG
  AAGACTCAGGCCCTGGAAGGCCGCTCAGCTGCCTCATGGAGGAACCTGCCCCGTATACAGATGGCACCGG
  TGCAGCGGCAGGAGGTGGGAACTGCAGGTTTGTGGAGTCTCCAAGCCAAGATCAGAGACTCCTGGTACAG
  CGACTCCGAAATCCTGAGGTTCGAGGATCCCTACAGACACCGCAGAATCGACCACATGGCCACCAGTCAC
  CGGAGCTGCCTGAAGGCTACGAGCAAAGGACAACAGTGCAGGGACAAGTTTACTTTTTGCACACGCAGAC
  TGGAGTCAGCACATGGCACGACCCCAGGATCCCCAGAGACCTTAACAGTGTGAACTGCGATGAACTTGGG
  CCACTGCCTCCAGGCTGGGAAGTCAGAAGCACAGTGTCGGGAAGAATCTATTTTGTAGATCACAATAATA
  GGACAACCCAGTTTACAGACCCACGGCTTCACCACATCATGAATCACCAGTGCCAACTCAAGGAGCCCAG
  CCAGCCGCTGCAGTTGCCCAGTGAGGGCTCCGTGGAGGACGAGGAGCTTCCTGCCCAGAGATATGAGAGA
  GATTTAGTCCAGAAGCTTAAAGTTCTCAGACATGAGCTCTCTCTTCAGCAGCCCCAAGCTGGTCATTGTC
  GCATAGAAGTTTCCAGAGAAGAAATATTTGAGGAGTCATATCGCCAGATCATGAAGATGCGACCAAAAGA
  CCTGAAGAAGCGCCTGATGGTGAAGTTCCGAGGGGAGGAAGGCTTGGACTATGGTGGGGTGGCTCGGGAG
  TGGCTGTATTTGTTGTGCCATGAAATGTTGAATCCGTATTATGGACTCTTCCAGTATTCCACGGACAATA
  TTTACACACTGCAAATCAACCCAGATTCTTCTATCAACCCTGACCATCTGTCTTACTTCCACTTTGTGGG
  TCGCATCATGGGTCTGGCTGTGTTCCATGGACACTACATAAACGGGGGCTTCACAGTTCCTTTCTACAAG
  CAGCTCCTGGGAAAACCCATCCAGCTGTCGGATCTGGAGTCAGTGGACCCAGAACTTCACAAGAGCTTGG
  TGTGGATTCTAGAGAATGACATCACGCCAGTGCTGGATCACACCTTCTGCGTGGAGCACAATGCTTTTGG
  GCGGATCCTTCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCTGTCACTGAGGAGAACAAGAAGGAG
  TATGTGCGGTTGTATGTGAACTGGAGGTTTATGAGAGGAATCGAAGCCCAGTTCCTAGCGCTTCAGAAAG
  GGTTTAACGAACTCATTCCCCAACACTTACTGAAGCCCTTTGACCAGAAGGAACTAGAGCTGATAATAGG
  CGGCCTGGATAAGATAGACCTGAATGACTGGAAGTCCAACACTCGGCTGAAGCACTGTGTGGCAGACAGC
  AACATCGTCAGGTGGTTCTGGCAGGCGGTGGAGACCTTCGATGAGGAGAGGAGAGCCAGGCTCCTGCAGT
  TTGTGACAGGATCCACACGAGTTCCTCTCCAAGGCTTCAAGGCTCTGCAAGGCGCGGCAGGGCCCCGGCT
  CTTCACCATCCACCTGATTGACGCCAACACCGACAACCTGCCCAAGGCCCACACCTGCTTTAATCGGATT
  GACATCCCACCCTATGAGTCCTATGAGAAGCTCTATGAGAAGCTGCTGACAGCGGTAGAGGAGACCTGTG
  GCTTTGCCGTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204842 representing NM_001109598
  Red=Cloning site Green=Tags(s)
  
  MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYV
  GKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTR
  DRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQ
  RLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELG
  PLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYER
  DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVARE
  WLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYK
  QLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKE
  YVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADS
  NIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRI
  DIPPYESYEKLYEKLLTAVEETCGFAVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109598
• ORF Size: 2184 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001109598.1, NP_001103068.1
• RefSeq Size: 5101
• RefSeq ORF: 2187
• Locus ID: 690516
• MW: 83.6 kDa