Nfkb2 (NM_001008349) Rat Tagged ORF Clone

CAT#: RR204858

Nfkb2 (Myc-DDK-tagged ORF) - Rat nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100 (Nfkb2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfkb2
• Synonyms: RGD1307189
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204858 representing NM_001008349
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGTTGCTACGATCCAGGTTTGGATGGCATCCCTGAATATGATGATTTTGAATTCAGCCCCTCCA
  TTGTGGAGCCCAAGGATCCAGCCCCAGAGACAGCTGATGGCCCCTATCTGGTGATTGTGGAACAGCCTAA
  ACAGCGAGGCTTCAGATTTCGATATGGCTGTGAAGGCCCCTCCCATGGAGGTCTGCCAGGTGCCTCCAGT
  GAGAAGGGCCGGAAGACCTATCCTACTGTCAAGATCTGTAACTACGAGGGGCCGGCCAAGATTGAGGTGG
  ACCTGGTGACACACAGTGACCCACCCCGTGCACATGCCCACAGTCTGGTGGGCAAGCAGTGCTCTGAGCT
  GGGGGTGTGTGCCGTGTCTGTGGGACCCAAGGACATGACTGCTCAATTTAATAATCTGGGTGTCCTGCAT
  GTAACCAAGAAGAACATGATGGAGATTATGATTCAGAAACTCCAGAGGCAGCGGCTCCGCTCCAAGCCTC
  AGGGCCTTACAGAGGCCGAGCGCCGGGAGCTGGAGCAGGAGGCCAAGGAGCTGAAGAAAGTCATGGATTT
  GAGCATTGTACGGCTACGCTTCTCAGCTTTCCTTCGAGCTAGTGATGGCTCCTTCTCCCTGCCCCTGAAG
  CCTGTGATCTCCCAGCCCATCCACGACAGCAAGTCTCCAGGGGCCTCAAACCTGAAGATTTCCCGAATGG
  ACAAAACAGCAGGTTCCGTGCGCGGTGGAGACGAAGTTTATTTGCTTTGCGATAAGGTGCAGAAAGATGA
  CATTGAGGTTCGTTTCTATGAGGATGACGAGAACGGATGGCAAGCCTTTGGGGACTTCTCTCCCACAGAC
  GTTCATAAACAGTATGCCATTGTGTTCCGGACACCGCCCTATCACAAGATGAAGATTGAGAGGCCTGTAA
  CGGTGTTCCTGCAGCTGAAACGCAAGCGTGGGGGCGATGTCTCCGACTCCAAACAGTTCACGTATTACCC
  TCTGGTGGAAGACAAGGAGGAAGTACAGAGGAAGCGGAGAAAGGCCTTGCCTACCTTCTCCCAGCCCTTC
  GGGGGCGGATCCCACATGGGTGGAGGTTCTGGGGGCTCCGCTGGGGGTTATGGAGGCGCTGGAGGAGGTG
  GCAGCCTCGGCTTTTTCTCCTCCTTCGCCTACAGCCCCTACCAATCCGGTGCAGCCCCAATGGGCTGCTT
  CCCGGGTGGGGGAGGTGGAGCGCAGATGGCCGGCTCTGGACGGGACGCGGAGGCTGGCGAGGCAGAGGAG
  CAGCCCAGAGCACCCTCCGAGGCCCCCCAGAGCGAGCAGCAGGCCCTGGACACGCTGCAGCGAGCTCGCG
  AGTACAACGCGCGCCTGTTCGGTCTGGCGCAGCGCAGCGCCCGAGCGTTGCTGGACTACGGCGTCACCGC
  GGACGCGCGTGCTCTGCTAGCGGGACAGCGCCACCTGCTGATGGCACAGGACGAGAACGGAGACACGCCG
  CTGCACCTGGCCATTATCCATGGGCAGACTGGTGTCATTGAGCAGATAGCCCAAGTCATTTATCATGCTC
  AGTACCTTGGGGTCATCAACCTCACCAATCACCTGCACCAGACACCTCTGCACCTGGCAGTAATCACTGG
  GCAGACACGGGTGGTGAGCTTCCTGCTGCAGGTGGGTGCAGACCCCACACTGCTGGATCGCCATGGTGAC
  TCAGCTGTCCACTTGGCTCTCCGGGCAGGTGCTGCAGCCCCAGACCTGCTGCAGGCAGTGTTGCACAGTG
  GAGCCCACGCTCTGCCCCAAATGTTACACATGCCTGATTTTGAGGGACTATACCCAGTACACCTGGCGGT
  CCATGCCCGAAGCCCTGAGTGCCTGGATCTGTTAGTTGACTGTGGAGCTGAAGTGGAGGCAGCAGAGAGG
  CAAGGAGGCCGAACCCCACTGCATCTAGCCACAGAGATGGAGGAGCTGGGGTTGGTCACCCATCTGGTCA
  CCAAGCTCCATGCTAATGTGAATGCCCGGACCTTTGCTGGAAACACACCCCTCCACCTGGCGGCTGGACT
  TGGATCCCCAACCCTCACCCGCCTCCTTCTAAAGGCTGGTGCTGACATCCATGCGGAGAATGAAGAGCCT
  CTCTGCCCACTGCCCTCACCCCCTACCTCTGGGAGCGATTCAGACTCTGAGGGGCCTGAGACAGATACCC
  AAAGAAACTTCCGAGGCCATACCCCTCTTGACCTCACTCGCAGTACCAAGGTGAAGACCCTGCTGTTGAA
  CGCTGCTCAGAACACCACGGAGCCACCCCTGGCCCCACCCAGCCCTGCAGGGCCAGGGCTGTCACTGGGG
  GATGCAGCCCTGCAGAACCTGGAGCAACTGCTAGATGGGCCAGAAGCCCAGGGCAGCTGGGCAGAGCTGG
  CAGAGAGACTGGGGCTGAGGAGCCTGGTGGACACATACAGGAAGACCCCCTCCCCCAGTGGCAGTCTCCT
  TCGTAGTTACAAGCTGGCTGGTGGAGACTTGGTGGGTCTATTGGAAGCCTTGTCCGACATGGGTCTTCAT
  GAGGGAGTTAGGCTGCTGAAGGCGCCTGAGACCCGAGACAAGCTGCCCAGCACAGAGGTGAAAGAAGACA
  GTGCCTACGGGAGCCAGTCAGTGGAGCAGGAGGCAGAGAAGCTGTGTCCACCCCCTGAGCCTCCAGGAGG
  GCTCTGCCACGGGCACCCCCAGCCTCAGGTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204858 representing NM_001008349
  Red=Cloning site Green=Tags(s)
  
  MDRCYDPGLDGIPEYDDFEFSPSIVEPKDPAPETADGPYLVIVEQPKQRGFRFRYGCEGPSHGGLPGASS
  EKGRKTYPTVKICNYEGPAKIEVDLVTHSDPPRAHAHSLVGKQCSELGVCAVSVGPKDMTAQFNNLGVLH
  VTKKNMMEIMIQKLQRQRLRSKPQGLTEAERRELEQEAKELKKVMDLSIVRLRFSAFLRASDGSFSLPLK
  PVISQPIHDSKSPGASNLKISRMDKTAGSVRGGDEVYLLCDKVQKDDIEVRFYEDDENGWQAFGDFSPTD
  VHKQYAIVFRTPPYHKMKIERPVTVFLQLKRKRGGDVSDSKQFTYYPLVEDKEEVQRKRRKALPTFSQPF
  GGGSHMGGGSGGSAGGYGGAGGGGSLGFFSSFAYSPYQSGAAPMGCFPGGGGGAQMAGSGRDAEAGEAEE
  QPRAPSEAPQSEQQALDTLQRAREYNARLFGLAQRSARALLDYGVTADARALLAGQRHLLMAQDENGDTP
  LHLAIIHGQTGVIEQIAQVIYHAQYLGVINLTNHLHQTPLHLAVITGQTRVVSFLLQVGADPTLLDRHGD
  SAVHLALRAGAAAPDLLQAVLHSGAHALPQMLHMPDFEGLYPVHLAVHARSPECLDLLVDCGAEVEAAER
  QGGRTPLHLATEMEELGLVTHLVTKLHANVNARTFAGNTPLHLAAGLGSPTLTRLLLKAGADIHAENEEP
  LCPLPSPPTSGSDSDSEGPETDTQRNFRGHTPLDLTRSTKVKTLLLNAAQNTTEPPLAPPSPAGPGLSLG
  DAALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRKTPSPSGSLLRSYKLAGGDLVGLLEALSDMGLH
  EGVRLLKAPETRDKLPSTEVKEDSAYGSQSVEQEAEKLCPPPEPPGGLCHGHPQPQVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008349
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008349.1, NP_001008350.1
• RefSeq Size: 3098 bp
• RefSeq ORF: 2697 bp
• Locus ID: 309452
• Cytogenetics: 1q54
• MW: 96.7 kDa