Vsig1 (NM_001037784) Rat Tagged ORF Clone

CAT#: RR204859

Vsig1 (Myc-DDK-tagged ORF) - Rat V-set and immunoglobulin domain containing 1 (Vsig1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vsig1
• Synonyms: MGC114516
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204859 representing NM_001037784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGTGTTTGCATTTTGGAAGGTCTTTCTGATCTTAAACTGCCTTGCAGGTCAGGTCAATATGGTGC
  AAGTGACCATCCCAGACACTTTTGTGAATGTGACTGTTGGATCTAATGTTACTCTTCTCTGCCTGTATAC
  CACCACTGTGACATCCCTGGAAAAGCTTTCAATCCAATGGTCTTTCTTCCACAATAAGGAAATGGAGCCA
  ATATCCATCTACTACTCTGAAGGTGGACAGGCTTCAGCTATTGGGCAATTCAAGGATCGAATAATAGGGG
  CTACTAATCCTGGTAATGCATCTATCACCATATTGCATATGCAGCCAGCAGACAGTGGAATTTACATATG
  TGATGTCAACAATCCTCCAGATTTTTTTGGAAAAAACCAAGGCATCCTTGACGTCACTGTCTTAGTCAAA
  CCTTCCAAGCCCTTTTGTTCCATCCAAGGAAGACCAGAAGCAGGCCACCCTATTTCCTTGTCTTGCCTTT
  CTGCCTTTGGAACACCATCTCCTGTGTATTACTGGTATAAGATTGAGGGGAACACCATTGTGCCAGTAAA
  AGAAAGCTTCAACTCAGCCACTGGAGTTTTGGATATTGGAAATCTAACAAATTTTGAAAAAGGTTATTAC
  CAGTGTACTGCCATCAACAGTCTTGGCAATAGTTCCTGTGAAATTGATTTAACCTCTTCAGATCCAGAAG
  TCGGAATCATCATTGGAGCCTTGGTCGGTGCCCTGACTGGAGCTGCTATCATCATCTGTGTCGTGTACTT
  TGCCAGGAACAAAGTTAAATCCAAGCAAAAGAATTTAAATTCCAGCACAGAGCTTGAGCCAATGACAAAA
  GTACACCATTCCCGACAAAATGAGGCTATTCCAGCGGGTGGCATCCAGCTAGAAGGAACTCTGCCATCTT
  CCATCCATGCCAGCCACAACACTGAACCTACCACTGCAGTCTTGGAGCCAGAATATGAGCCTAATCCCCC
  GCTGGAAACTGCCACACAGCCTGACCCTGAACCAGAGGGTTCAGGGCCGATGCCAGTGCCTGAGACGGAG
  ATTCAACTGCAGCCAGAGATGGAGCTGGAACCGGAGACAGAGCCTGAGCCTGAGCCTGAGCCTGAGCCTC
  AACCTGAGCTTGAGTCAGAGCTTGAGCCCGACCCCCAGTCTGGAGTAATAGTTGAGCCTATGCGTGAGGA
  GGAAAAGGAAACAGTTAAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204859 representing NM_001037784
  Red=Cloning site Green=Tags(s)
  
  MMVFAFWKVFLILNCLAGQVNMVQVTIPDTFVNVTVGSNVTLLCLYTTTVTSLEKLSIQWSFFHNKEMEP
  ISIYYSEGGQASAIGQFKDRIIGATNPGNASITILHMQPADSGIYICDVNNPPDFFGKNQGILDVTVLVK
  PSKPFCSIQGRPEAGHPISLSCLSAFGTPSPVYYWYKIEGNTIVPVKESFNSATGVLDIGNLTNFEKGYY
  QCTAINSLGNSSCEIDLTSSDPEVGIIIGALVGALTGAAIIICVVYFARNKVKSKQKNLNSSTELEPMTK
  VHHSRQNEAIPAGGIQLEGTLPSSIHASHNTEPTTAVLEPEYEPNPPLETATQPDPEPEGSGPMPVPETE
  IQLQPEMELEPETEPEPEPEPEPQPELESELEPDPQSGVIVEPMREEEKETVKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037784
• ORF Size: 1212 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037784.1, NP_001032873.1
• RefSeq Size: 1562 bp
• RefSeq ORF: 1215 bp
• Locus ID: 315920
• Cytogenetics: Xq33
• MW: 43.9 kDa