Fh (NM_017005) Rat Tagged ORF Clone

CAT#: RR204878

Fh (Myc-DDK-tagged ORF) - Rat fumarate hydratase 1 (Fh1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fh
• Synonyms: Fh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204878 representing NM_017005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGCGCATTCTGTCTCCTCGCTCGCTCGCGTCGCTTCCCGCGGGTCCCCTCCGCCGGTGCTGTAT
  TGTCAGGGGAAGCGGCCACCCTCCCACGGTGTGCTCCGAACGTCGTGCGAATGGCAAGCCAAAATTCCTT
  CCGTATAGAATACGACACCTTTGGTGAACTGAAGGTCCCAACCGATAAGTATTATGGTGCTCAGACTGTA
  AGATCTACGATGAACTTTAAGATTGGAGGTGCGACGGAGAGAATGCCAATCCCAGTCATTAAAGCATTTG
  GCATCTTGAAGCGAGCTGCTGCTGAAGTGAACCAGGAGTATGGTCTCGATCCCAAGATTGCTAGTGCAAT
  AATGAAGGCCGCAGATGAGGTAGCTGAAGGTAAATTAAACGATCACTTTCCTCTGGTGGTATGGCAGACT
  GGGTCAGGAACCCAGACGAACATGAATGTAAATGAAGTCATTAGCAACAGGGCAATCGAAATGCTAGGAG
  GTGAACTTGGCAGCAAGAAGCCCGTGCACCCCAATGATCACGTTAACAAAAGCCAGAGCTCGAATGACAC
  CTTCCCCACAGCAATGCACATTGCTGCTGCACTGGAAGTTCACCAGGTCCTGTTGCCAGGATTACAGAAG
  CTGCACGATGCCCTCAGTGCAAAATCCAAAGAGTTCGCGCAGGTCATAAAAATTGGGCGGACTCATACGC
  AGGACGCTGTCCCTCTTACTCTTGGACAGGAGTTCAGTGGTTATGTTCAGCAAGTCCAGTATGCGATGGA
  GAGGATAAAAGCTGCCATGCCCAGAATCTACGAGCTCGCTGCTGGCGGCACTGCCGTCGGCACGGGACTA
  AACACCAGGATCGGCTTCGCAGAAAAGGTGGCTGCAAAAGTCGCAGCACTCACAGGTTTGCCTTTTGTCA
  CTGCCCCGAATAAGTTTGAAGCTCTGGCCGCTCATGATGCTCTGGTGGAGCTTAGTGGAGCCATGAACAC
  TACGGCCTGCAGTCTGATGAAGATTGCCAATGACATTCGCTTCCTCGGTTCTGGTCCTCGGTCCGGTTTG
  GGGGAGCTGATCTTGCCTGAAAATGAGCCAGGGAGCAGCATAATGCCAGGAAAGGTGAACCCTACTCAGT
  GTGAGGCGATGACCATGGTTGCAGCCCAAGTCATGGGGAATCATGTCGCTGTTACTGTTGGAGGCAGCAA
  TGGACATTTCGAACTGAATGTTTTTAAACCGATGATGATTAAGAATGTGTTGCACTCAGCCAGGCTACTG
  GGAGATGCTTCAGTGTCCTTCACAGAGAACTGTGTGGTAGGGATCCAGGCCAACACAGAGAGGATCAACA
  AGCTAATGAACGAGTCTTTAATGTTGGTTACAGCTCTTAATCCGCATATAGGGTATGACAAAGCAGCGAA
  GATTGCCAAGACTGCACACAAGAACGGATCAACCTTAAAGGAAACGGCTATTGAACTTGGCTATCTCACA
  GCAGAGCAGTTTGATGAGTGGGTGAAACCCAAGGACATGCTGGGTCCAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204878 representing NM_017005
  Red=Cloning site Green=Tags(s)
  
  MNRAFCLLARSRRFPRVPSAGAVLSGEAATLPRCAPNVVRMASQNSFRIEYDTFGELKVPTDKYYGAQTV
  RSTMNFKIGGATERMPIPVIKAFGILKRAAAEVNQEYGLDPKIASAIMKAADEVAEGKLNDHFPLVVWQT
  GSGTQTNMNVNEVISNRAIEMLGGELGSKKPVHPNDHVNKSQSSNDTFPTAMHIAAALEVHQVLLPGLQK
  LHDALSAKSKEFAQVIKIGRTHTQDAVPLTLGQEFSGYVQQVQYAMERIKAAMPRIYELAAGGTAVGTGL
  NTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGL
  GELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLL
  GDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLT
  AEQFDEWVKPKDMLGPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017005
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017005.1, NM_017005.2, NP_058701.2
• RefSeq Size: 1603
• RefSeq ORF: 1524
• Locus ID: 24368
• MW: 54.5 kDa
• Gene Summary: precursor for mitochondrial and cytosolic forms [RGD, Feb 2006]