Alad (NM_012899) Rat Tagged ORF Clone

CAT#: RR204880

Alad (Myc-DDK-tagged ORF) - Rat aminolevulinate, delta-, dehydratase (Alad), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alad
• Synonyms: ALADR; aminolevulinatedelta-dehydratase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204880 representing NM_012899
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCACCAGTCCGTTCTGCACAGTGGCTACTTTCACCCACTGCTTCGGGCCTGGCAGACCACCCCCT
  CCACCGTCAGTGCCACCAACCTCATCTATCCCATCTTTGTCACGGATGTTCCTGATGATGTCCAGCCCAT
  TGCCAGCCTCCCGGGAGTGGCCAGGTATGGCGTAAACCAGCTAGAGGAGATGCTGAGACCTCTGGTGGAA
  GCCGGCCTTCGCTGTGTCCTGATCTTTGGCGTCCCCAGCAGAGTTCCCAAGGATGAACAGGGCTCTGCAG
  CTGACTCAGAGGACTCCCCAACTATTGAGGCTGTCCGTCTGCTGAGGAAGACCTTCCCTACCCTCCTAGT
  GGCCTGTGATGTCTGCCTGTGCCCCTACACCTCCCATGGCCACTGTGGCCTCCTGAGTGAAAATGGAGCC
  TTCCTAGCAGAGGAAAGCCGACAACGGTTGGCAGAGGTGGCACTGGCCTATGCCAAGGCAGGTTGTCAGG
  TTGTAGCTCCATCAGACATGATGGATGGACGAGTTGAAGCCATCAAGGCTGCCCTGCTAAAACATGGACT
  TGGCAACAGGGTCTCTGTGATGAGCTATAGTGCCAAATTTGCCTCCTGTTTCTATGGTCCTTTCCGGGAT
  GCAGCTCAGTCAAGCCCAGCTTTCGGAGACCGACGCTGTTATCAGCTGCCTCCTGGAGCCCGTGGCCTGG
  CCCTCCGAGCAGTGGCCCGGGACATTCAAGAGGGAGCTGACATACTCATGGTAAAGCCGGGATTACCCTA
  CCTGGATATGGTGCAGGAGGTGAAGGACAAGCACCCCGAGCTCCCCCTCGCAGTATACCAGGTGTCCGGA
  GAGTTTGCCATGTTGTGGCACGGAGCCAAGGCTGGGGCCTTTGATCTCAGGACTGCTGTACTGGAGTCCA
  TGACGGCCTTCCGCAGAGCCGGTGCTGACATTATCATCACCTACTTTGCACCCCAGCTGTTGAAGTGGCT
  GAAGGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204880 representing NM_012899
  Red=Cloning site Green=Tags(s)
  
  MHHQSVLHSGYFHPLLRAWQTTPSTVSATNLIYPIFVTDVPDDVQPIASLPGVARYGVNQLEEMLRPLVE
  AGLRCVLIFGVPSRVPKDEQGSAADSEDSPTIEAVRLLRKTFPTLLVACDVCLCPYTSHGHCGLLSENGA
  FLAEESRQRLAEVALAYAKAGCQVVAPSDMMDGRVEAIKAALLKHGLGNRVSVMSYSAKFASCFYGPFRD
  AAQSSPAFGDRRCYQLPPGARGLALRAVARDIQEGADILMVKPGLPYLDMVQEVKDKHPELPLAVYQVSG
  EFAMLWHGAKAGAFDLRTAVLESMTAFRRAGADIIITYFAPQLLKWLKEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012899
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012899.1, NM_012899.2, NP_037031.1
• RefSeq Size: 1278
• RefSeq ORF: 993
• Locus ID: 25374
• MW: 36 kDa
• Gene Summary: catalyzes the conversion of 5-aminolevulinate to porphobilinogen in heme biosynthesis [RGD, Feb 2006]