B4galt7 (NM_001031661) Rat Tagged ORF Clone

CAT#: RR204953

B4galt7 (Myc-DDK-tagged ORF) - Rat xylosylprotein beta1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4galt7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B4galt7
• Synonyms: MGC116242
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204953 representing NM_001031661
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCCTCCCGGAGGAAAGCGGCGCAGCTGCCCTGGGAGGATGGCAGGGCCAGGCTGCTTCCTGGAG
  GCCTCCGCCGGAAATGCTCCATCTTCCACCTCTTCATTGCCTTTCTCCTGTTGGTCTTCTTCTCCCTGCT
  CTGGCTGCAGCTCAGCTGTTCTGGAGATGTGGCCCAGGTGACCAGGGGACAAGGGCAGGAGACCTCGGGT
  CCACCCCGAGCTTGTCCCCCAGAACCGCCCCCTGAGCACTGGGAAGAAGATGAGTCATGGGGGCCTCACC
  GGTTGGCAGTGCTGGTGCCCTTTCGGGAACGCTTTGAGGAGCTGCTGGTCTTTGTGCCCCACATGCACCG
  CTTCCTAAGCAGGAAGAAGATCCAGCACCACATCTATGTGCTCAACCAGGTGGATCACTTCAGGTTCAAT
  CGGGCAGCACTCATCAATGTGGGCTTCCTGGAGAGCAGCAACAGCACAGACTACATCGCCATGCACGATG
  TGGACCTACTCCCTCTCAATGAGGAGCTGGACTATGGCTTCCCGGAGGCTGGGCCCTTCCATGTGGCTTC
  CCCAGAGCTCCACCCTCTCTACCACTACAAGACCTATGTGGGTGGCATTCTGCTGCTGTCCAAACAGCAC
  TACCAGCTGTGCAACGGGATGTCCAACCGCTTCTGGGGCTGGGGCCGAGAGGACGATGAATTCTACCGGC
  GCATCAAAGGAGCTGGTCTCCAGCTTTTCCGCCCCTTGGGGATCACAACTGGGTACCAGACATTTCGCCA
  CTTGCATGACCCAGCCTGGCGGAAGAGGGACCAGAAACGCATTGCGGCTCAAAAACAGGAACAGTTCAAG
  GTGGACCGGGAGGGAGGCCTGAACACTGTGAGGTACCGGGTGGATTCCCGCACGGCACTGTCCGTAGGAG
  GGGCCCCCTGCACTGTTCTCAATGTCATGCTGGACTGCGATAAGACAGCCACTCCATGGTGCACATTTGG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204953 representing NM_001031661
  Red=Cloning site Green=Tags(s)
  
  MLPSRRKAAQLPWEDGRARLLPGGLRRKCSIFHLFIAFLLLVFFSLLWLQLSCSGDVAQVTRGQGQETSG
  PPRACPPEPPPEHWEEDESWGPHRLAVLVPFRERFEELLVFVPHMHRFLSRKKIQHHIYVLNQVDHFRFN
  RAALINVGFLESSNSTDYIAMHDVDLLPLNEELDYGFPEAGPFHVASPELHPLYHYKTYVGGILLLSKQH
  YQLCNGMSNRFWGWGREDDEFYRRIKGAGLQLFRPLGITTGYQTFRHLHDPAWRKRDQKRIAAQKQEQFK
  VDREGGLNTVRYRVDSRTALSVGGAPCTVLNVMLDCDKTATPWCTFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031661
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031661.1, NP_001026831.1
• RefSeq Size: 1591 bp
• RefSeq ORF: 984 bp
• Locus ID: 364675
• Cytogenetics: 17p14
• MW: 37.7 kDa