Alox5 (NM_012822) Rat Tagged ORF Clone

CAT#: RR204994

Alox5 (Myc-DDK-tagged ORF) - Rat arachidonate 5-lipoxygenase (Alox5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alox5
• Synonyms: LOX5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204994 representing NM_012822
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCCTACACTGTCACCGTAGCCACCGGTAGCCAGTGGTTCGCGGGCACCGACGACTACATTTACC
  TCAGCCTCATTGGCTCTGCAGGCTGCAGTGAGAAGCATCTTCTTGACAAAGCTTTCTACAATGACTTCGA
  GCGCGGCGGTCGCGACTCCTATGACGTCACTGTGGATGAAGAACTGGGTGAGATCTACCTAGTCAAAATC
  GAGAAGCGCAAATACAGGCTCCATGATGACTGGTACTTGAAATACATCACACTGAAGACACCCCACGACT
  ACATAGAGTTCCCTTGTTATCGTTGGATCACAGGCGAGGGCGAGATTGTCCTGAGGGATGGATGTGCAAA
  ATTGGCCCGAGATGACCAAATCCACATCCTCAAGCAGCACAGGCGGAAAGAACTGGAAACACGTCAGAAA
  CAGTATCGATGGATGGAGTGGAACCCCGGCTTCCCTTTGAGTATTGATGCTAAATGCCACAAGGATCTGC
  CCCGAGATATCCAGTTTGATAGTGAAAAGGGAGTGGACTTTGTTCTGAACTACTCAAAAGCGATGGAGAA
  CCTGTTCATCAATCGCTTCATGCACATGTTCCAGTCTTCCTGGCATGACTTTGCTGACTTTGAGAAAATC
  TTCGTCAAAATCAGCAACACTATTTCTGAGAGAGTCAAGAACCACTGGCAAGAGGACCTCATGTTTGGCT
  ACCAGTTCCTGAATGGCTGCAACCCAGTACTCATCAAGCGCTGCACAGAGTTGCCTAAGAAGCTCCCAGT
  GACCACAGAAATGGTGGAGTGCAGCCTAGAGCGGCAGCTCAGTTTAGAACAGGAAGTACAGGAAGGGAAC
  ATTTTCATCGTTGATTACGAACTACTGGATGGCATTGATGCTAACAAAACTGACCCCTGTACACACCAGT
  TCCTGGCCGCCCCCATCTGCCTGCTGTATAAGAACCTAGCCAACAAGATTGTTCCCATCGCCATCCAGCT
  CAACCAAACCCCTGGAGAGAAGAACCCAATTTTCCTCCCTACGGACTCAAAATACGACTGGCTTTTGGCC
  AAAATCTGGGTGCGTTCAAGTGACTTCCATATCCATCAAACAATCACCCATCTTCTCCGCACACATCTGG
  TGTCTGAGGTGTTCGGTATTGCCATGTACCGCCAGCTGCCTGCTGTGCACCCCCTTTTCAAGCTGCTGGT
  AGCCCATGTGAGGTTCACCATTGCCATCAACACTAAGGCCCGGGAACAGCTTAACTGTGAGTACGGCCTT
  TTTGACAAGGCCAATGCCACCGGAGGTGGTGGGCACGTGCAGATGGTGCAGAGAGCTGTCCAGGACCTGA
  CCTATTCCTCCCTGTGCTTCCCGGAGGCCATCAAGGCCCGGGGCATGGACAACACCGAGGACATCCCCTA
  CTACTTCTATCGTGATGATGGACTGCTCGTGTGGGAAGCTATCCAGTCGTTCACAACTGAGGTGGTAAGC
  ATCTACTATGAGGATGACCAGGTGGTGGAGGAGGACCAGGAACTGCAGGACTTCGTGAAGGATGTTTACG
  TTTATGGCATGCGGGGCAGAAAGGCCTCAGGTTTCCCCAAGTCCATCAAGAGCAGGGAGAAATTGTCTGA
  GTACCTGACGGTGGTGATCTTCACAGCCTCGGCCCAGCATGCAGCTGTAAACTTTGGCCAGTATGACTGG
  TGCTCCTGGATCCCCAACGCTCCTCCAACTATGCGGGCCCCACCACCCACGGCCAAGGGTGTGGTCACCA
  TCGAGCAGATTGTGGATACTCTACCAGACCGTGGCCGCTCATGTTGGCATCTAGGTGCAGTGTGGGCCTT
  GAGCCAGTTTCAAGAGAATGAGCTGTTTCTGGGCATGTACCCAGAGGAGCATTTCATCGAGAAGCCAGTG
  AAAGAAGCCATGATTCGATTCCGCAAGAACCTGGAGGCCATCGTCAGCGTGATTGCCGAGCGCAATAAGA
  ACAAAAAGCTCCCCTACTACTACCTGTCACCAGACAGATTCCAAACAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204994 representing NM_012822
  Red=Cloning site Green=Tags(s)
  
  MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYNDFERGGRDSYDVTVDEELGEIYLVKI
  EKRKYRLHDDWYLKYITLKTPHDYIEFPCYRWITGEGEIVLRDGCAKLARDDQIHILKQHRRKELETRQK
  QYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKI
  FVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTELPKKLPVTTEMVECSLERQLSLEQEVQEGN
  IFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGEKNPIFLPTDSKYDWLLA
  KIWVRSSDFHIHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLNCEYGL
  FDKANATGGGGHVQMVQRAVQDLTYSSLCFPEAIKARGMDNTEDIPYYFYRDDGLLVWEAIQSFTTEVVS
  IYYEDDQVVEEDQELQDFVKDVYVYGMRGRKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQYDW
  CSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPV
  KEAMIRFRKNLEAIVSVIAERNKNKKLPYYYLSPDRFQTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012822
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012822.1, NP_036954.1
• RefSeq Size: 2535
• RefSeq ORF: 2013
• Locus ID: 25290
• MW: 77.9 kDa
• Gene Summary: catalyzes the conversion of arachidonate to leukotriene A4 in leukotriene metabolism [RGD, Feb 2006]