C4b (NM_001002805) Rat Tagged ORF Clone

CAT#: RR205006

C4b (Myc-DDK-tagged ORF) - Rat complement component 4, gene 2 (C4-2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C4b
• Synonyms: C4-2; C4l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205006 representing NM_001002805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTCCTCTGGGGCCTGGCCTGGGCATTCAGCTTCTTCGCCTCCTCTCTGCAGAAGCCCAGGTTGC
  TCCTGTTCTCCCCTTCTGTGGTTAATTTGGGGACCCCCCTGTCAGTAGGGGTGCAGCTCCTGGATGCCCC
  TGCAGGACAGGAGGTGAAAGGCTCAGTGTACCTCAGAAACCCAACAAGCGGTCCCTGCTCCCCAAAGAAG
  GACTTTAAGCTCAGCTCGGGAAATGACTTTGTGCTTCTCAGACTTGAGGTCCCACTGGAAGATGTGAGGA
  ATTGTGGCCTCTTTGACCTACGCAGAGCCCCCCACATCCAGCTGGTGGCTTACTCTCCGTGGCTAAAGAA
  CACAGCACCCAAAGCCACCGAGACTCAGGGGGTCAACCTGCTTTTCTCTTCCCGACGAGGCCACATCTTT
  GTGCAGACCGATCAGCCTATCTATAACCCTGGGCAGCGGGTACGTTATCGGGTCTTTGCACTGGATCAAA
  AGATGCGTCCATCTACGGACACCCTCACGGTCACAGTAGAGAACTCCCATGGCCTCCGTGTACGCAAGAA
  GGTGGTATTTGCCCCCACATCCATCTTCACAGATGACTTCGTAATTCCAGACATCTCAGAGCCGGGGACC
  TGGAAGATCTCAGCTAGGTTCTCAGATGGCCTCGAGTCCAACAGAAGCACCCACTTTGAAGTGAAGAAAT
  ATGTCCTTCCCAACTTTGAGGTGAAGCTTACTCCTTGGAAGCCATATATCCTGACAGTGCCTAGCTACCG
  TGAAGAAATCCAGTTAGACATCCAGGCCAGATACGTCTACGGGAAGCCCGTGCAGGGCGTGGCATATACC
  CGCTTCGCACTCATGGATGAGCAGGGGAAGAAGACTTTCCTTCGGGGCCTAGAGACCCAGACCAAGTTGG
  TGGAAGGCCAGACTCACATCTCCATCTCAAGGGACCAGTTCCAGGCTGCCCTGGGTAAAGTGAATACTGA
  GATCGGAGACCTGGAGGGGCTGCGGCTCTACGCTGCTGTAGCTGTCATTGAGTCTCCAGGAGGAGAGATG
  GAGGAAGCAGAGCTCACATCCTGGCCCTTCGTGTCATCTGCCTTCTCCTTGGATCTCAGCCACACCAAGC
  AGCATCTTGTGCCCGGGGCCCCCTTCCTGCTGCAGGCCCTGGTTCGAGAAATGTCAGGCTCTGAAGCCTC
  TGATGTTCCTGTCAAAGTCTCTGCCACATTGTTATCTGGGTCCGACTCGAAAGTCCTTGACTTTCAACAG
  AACACCAACGGGATTGGCCAAGTCAGCTTTTCTATCCATGTCCCGCCAACCATCACAGAACTTCGACTCT
  TGGTATCTGCCGGTTCCCTCTACCCAGCTGTAGCCAAGCTCACTGTGCAAGCCCCACCTTCAAGAGGCCC
  TGGCTTTCTGTCCATTGAGCCACTAGACCTTCGGTCCCCTCGTGTGGGGGACACCTTCATCCTGAACCTT
  CGAACCGTGGGCATCCCTGTGCCTACCTTCTCTCATTACTACTACATGATCATCTCCAGAGGCCAGATCA
  TGGCCATGAGTCGGGAGCCCAGGACGACCCTGACCTCCATCTCTGTCTTGGTGGACCATCACCTGGCTCC
  CTCTTTCTACTTTGTGGCCTACTTCTATCACCAAGGACTCCCAGTGGCCAACTCCCTGCTCATCAATGTC
  CAGCCTGGGGACTGTGAGGGCAAGCTGGAATTGAAGGTGGACGGTGCCAAGGAGTATCGAAATGGGGAGA
  GCATGAAGCTCCAACTTCAAACTGACTCCGAAGCCCTGGTGGCACTGGGAGCTGTGGACACAGCTCTGTA
  TGCTGTGGGTGGCCGGTCTCACAAACCCCTCGACATGAGCAAGGTCTTTGAAGTAATGAACAGTTATAAC
  CTTGGCTGTGGTCCTGGAGGTGGGGACGATGCCCTTCAGGTGTTCCAGACTGCTGGTTTGGCCTTTTCTG
  ACGGTGATCGACTAACTCAGACCAAAGAGGACTTGAGCTGTCCCAAGGAGAAGATAAGTCGGCAAAAGAG
  AAATGTTAACTTCCAGAAGGCTATTAGCGAGAAGTTGGGCCAGTATTCCTCTCCAGACACCAAGCGCTGC
  TGCCAGGATGGGATGACCAAGCTGCCCATGGCACGCACCTGCGAGCAGCGGGCAGCCCGAGTGCCTCAGC
  CGGCCTGCCGGGAGCCCTTCTTGTCCTGTTGCAAGTTTGCTGAGGACCTCCGCAGGAACCAGACCAGGAG
  CCAGGCGGGCCTCGCCCGAGCCCAGAACGTGATGCAGGAGGAGGATCTGATAGATGAAGATGACATTCTT
  GTGCGCAGCTTCTTCCCAGAGAACTGGCTCTGGAAAGTGGAACCTGTGGACCGCTCCAAACTGTTGACTG
  TGTGGCTCCCTGACTCTCTGACCACATGGGAGATTCATGGTGTGAGCCTGTCCAAAAGCAAAGGCCTGTG
  TGTAGCCAAGCCAACTCGCGTGCGAGTGTTCCGAGAATTCCACCTGCACCTGCGCCTGCCCATCTCTGTC
  CGGCGCTTCGAGCAGCTTGAATTACGGCCCGTTCTCTACAACTATCTGAGTGAGGACGTGACTGTGAGTG
  TCCATGTGTCCCCAGTGGAGGGGCTGTGCCTGGCTGGTGGTGGATTGCTGGCCCAGCAGGTATCAGTGCC
  TGCAGGCTCCGCCCGGCCTGTGGCCTTCTCTGTGGTACCCACAGCTGCTGCCAGCATGCCCCTGAAAGTG
  GTGGCTCGGGGGTCTTTTGATATAGGGGATGCTGTGTCTAAGATTCTCCAAATTGAGAAGGAAGGGGCCA
  TCCACAGAGAAGAGATAGTCTACAACCTCGACCCCCTAAATAACCTTGGTCGGAGTTTGGAAATCCCTGG
  CAGCTCAGACCCCAACGTCATTCCTGATGGAGATTTCAGCAGCTTCGTCAGGGTTACAGCCTCCGAACCC
  TTGGAGACTTTGGGCTCTGAAGGCGCCTTGTCCCCAGGGGGAGTGGCCTCCCTCCTGAAGCTCCCCACGG
  GCTGCGCAGAACAAACCATGGTCCTTTTGGCCCCTACCCTGACTGCTTCCCGCTACCTGGACAGGACCGA
  GCAGTGGAGCAAACTGCCCCCTGAGACAAAGGACCATGCTGTGGATCTGATCCAGAAAGGCCACATGCGG
  ATCCAGCAGTTTAGGAAGAAAGATGGCTCCTTTGGGGCGTGGTTAGACCGGGACAGCAGCACCTGGCTCA
  CGGCCTTTGTGCTGAAGATTCTGAGTCTGGCCCAGGAACAGATTGGTGACTCCCCCGAGAAGCTGCAAGA
  GACCGCCGGGTGGCTGCTGGGCCAGCAGCTGGACGATGGCTCATTCCACGACCCGTGTCCGGTCATTCAA
  AGAGGCATGCAGGGGGGCTTGGTGGGGACCGACGAGACAGTGGCACTGACCGCCTTTGTGGTCATCGCCC
  TTCACCACGGGCTGGCCGTCTTCCAAGACGAGAACTCGCAGCAGCTGAAGAAGAGGGTGGAAGCCTCCAT
  CATGAAGGCAAACTCGTTCTTGGGGCAGAAGGCAAGTGCTGGGCTCCTGGGTGCACACGCATCCGCCATC
  ACAGCCTATGCCCTGACACTGACCAAGGCCTCGGAGGACCTGCGGAATGTTGCCCACAACAGCCTGATGG
  CCATGGCTGAGGAAACAGGGGAAAACCTCTACTGGGGCTTAGCCATTGGCTCTCAGGACAACGTTGTGTC
  ATCCACCGCAGCCCCTCGTAATCCCTCAGAACCTGTGCCCCAGGCCCCAGCCCTGTGGATCGAAACCACA
  GCCTACGGCCTGCTACATCTGCTGCTGCGGGAGGGGAAGGGAGAAATGGCTGACAAGGCGGCAACCTGGC
  TCACACACCAGGGAAGCTTCCAAGGGGGATTCCGCAGTACCCAGGACACTGTGGTCGCCCTGGATGCCCT
  GTCTGCATACTGGATCGCTTCACACACCACCGAGGAGAAGGCACTCAATGTGACTCTCAGCTCCATGGGC
  CGCAATGGGTACAAGTCCCACCTGCTACAGCTGAACAACCACCAAGTCAAGGGCCTGGAGGAGGAGCTGA
  AGTTCTCCCTGGGCAGCACCATCAATGTGAAAGTGGGAGGAAATAGCAAAGGCACCTTAAAGATCCTTCG
  TACCTACAATGTCCTGGACATGAAGAACACAACATGCCAAGACCTTCGGATAGAAGTGACAATCACAGGC
  TATGTGGAATATACAAGGGAGGCCAACGAAGACTATGAATACGACTATGATATGCCAGCCGCAGATGACC
  CCAGTGTCCACTCGCAGCCTGTGACACCCCTGCAGCTGTTTGAGGGTCGTCGGAGCCGCCGCAGGAGGGA
  GGCCCCCAAGGCTGCGGATGAGCAGGAGTCCAGAGTGCAGTACACCGTGTGCATCTGGCGGAACGGTAAC
  CTGGGGCTGTCTGGGATGGCCATTGCTGACATCACCCTCCTGAGTGGATTCCAAGCCCTGAGGGCTGACC
  TGGAGAAGCTGACCTCCCTCTCTGACCGTTACGTGAGTCACTTTGAGACCGACGGGCCCCACGTCCTACT
  GTACTTTGACTCGGTCCCGACCACCCGGGAGTGTGTGGGCTTTGGAGCTTTGCAGGAGGTGGCCGTGGGG
  CTGGTGCAGCCAGCCAGTGCTGTCCTGTATGACTACTACAGCCCTGATCACAAGTGCTCTGTGTTCTATG
  CTGGACCCACCAAGAGCAAGCTCCTCTCCACCTTGTGCTCTGCAGACGTCTGCCAGTGCGCTGAAGGGAA
  GTGTCCTCGACAGCGAAGGTCACTGGAGCGAGGGGTGGAGGACAAGGATGGTTACCGGATGAAGTTCGCC
  TGCTATTATCCCCGAGTGGAGTATGGCTTCCAGGTTAAGGTTCTCCGAGAAGACAGCAGATCTGCCTTCC
  GCCTCTTTGAGACCAAGATCACCCAAGTCCTGCATTTCACAAAGGATGCCAAGGCCTCCATAGGTCAGAC
  CCGCAACTTTCTGGTCCGGGCTTCTTGCCGCCTTCGTTTGGAGCCTAGCAAAGAGTACTTAATCATGGGG
  ATGGACGGAGTCACCAGTGACCTCAAGGGAGACCCCCAGTACTTGCTGGACTCAAACAGTTGGATCGAGG
  AGATGCCATCTGAGCGCCTGTGCAGGAGCACCCGTCAGCGGGCAGCCTGTGGCCAGCTCAATGACTTCCT
  GCAGGAGTACAGAAGCCAGGGGTGCCAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205006 representing NM_001002805
  Red=Cloning site Green=Tags(s)
  
  MRLLWGLAWAFSFFASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPAGQEVKGSVYLRNPTSGPCSPKK
  DFKLSSGNDFVLLRLEVPLEDVRNCGLFDLRRAPHIQLVAYSPWLKNTAPKATETQGVNLLFSSRRGHIF
  VQTDQPIYNPGQRVRYRVFALDQKMRPSTDTLTVTVENSHGLRVRKKVVFAPTSIFTDDFVIPDISEPGT
  WKISARFSDGLESNRSTHFEVKKYVLPNFEVKLTPWKPYILTVPSYREEIQLDIQARYVYGKPVQGVAYT
  RFALMDEQGKKTFLRGLETQTKLVEGQTHISISRDQFQAALGKVNTEIGDLEGLRLYAAVAVIESPGGEM
  EEAELTSWPFVSSAFSLDLSHTKQHLVPGAPFLLQALVREMSGSEASDVPVKVSATLLSGSDSKVLDFQQ
  NTNGIGQVSFSIHVPPTITELRLLVSAGSLYPAVAKLTVQAPPSRGPGFLSIEPLDLRSPRVGDTFILNL
  RTVGIPVPTFSHYYYMIISRGQIMAMSREPRTTLTSISVLVDHHLAPSFYFVAYFYHQGLPVANSLLINV
  QPGDCEGKLELKVDGAKEYRNGESMKLQLQTDSEALVALGAVDTALYAVGGRSHKPLDMSKVFEVMNSYN
  LGCGPGGGDDALQVFQTAGLAFSDGDRLTQTKEDLSCPKEKISRQKRNVNFQKAISEKLGQYSSPDTKRC
  CQDGMTKLPMARTCEQRAARVPQPACREPFLSCCKFAEDLRRNQTRSQAGLARAQNVMQEEDLIDEDDIL
  VRSFFPENWLWKVEPVDRSKLLTVWLPDSLTTWEIHGVSLSKSKGLCVAKPTRVRVFREFHLHLRLPISV
  RRFEQLELRPVLYNYLSEDVTVSVHVSPVEGLCLAGGGLLAQQVSVPAGSARPVAFSVVPTAAASMPLKV
  VARGSFDIGDAVSKILQIEKEGAIHREEIVYNLDPLNNLGRSLEIPGSSDPNVIPDGDFSSFVRVTASEP
  LETLGSEGALSPGGVASLLKLPTGCAEQTMVLLAPTLTASRYLDRTEQWSKLPPETKDHAVDLIQKGHMR
  IQQFRKKDGSFGAWLDRDSSTWLTAFVLKILSLAQEQIGDSPEKLQETAGWLLGQQLDDGSFHDPCPVIQ
  RGMQGGLVGTDETVALTAFVVIALHHGLAVFQDENSQQLKKRVEASIMKANSFLGQKASAGLLGAHASAI
  TAYALTLTKASEDLRNVAHNSLMAMAEETGENLYWGLAIGSQDNVVSSTAAPRNPSEPVPQAPALWIETT
  AYGLLHLLLREGKGEMADKAATWLTHQGSFQGGFRSTQDTVVALDALSAYWIASHTTEEKALNVTLSSMG
  RNGYKSHLLQLNNHQVKGLEEELKFSLGSTINVKVGGNSKGTLKILRTYNVLDMKNTTCQDLRIEVTITG
  YVEYTREANEDYEYDYDMPAADDPSVHSQPVTPLQLFEGRRSRRRREAPKAADEQESRVQYTVCIWRNGN
  LGLSGMAIADITLLSGFQALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGALQEVAVG
  LVQPASAVLYDYYSPDHKCSVFYAGPTKSKLLSTLCSADVCQCAEGKCPRQRRSLERGVEDKDGYRMKFA
  CYYPRVEYGFQVKVLREDSRSAFRLFETKITQVLHFTKDAKASIGQTRNFLVRASCRLRLEPSKEYLIMG
  MDGVTSDLKGDPQYLLDSNSWIEEMPSERLCRSTRQRAACGQLNDFLQEYRSQGCQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002805
• ORF Size: 5211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002805.1, NM_001002805.2, NP_001002805.1
• RefSeq Size: 5354 bp
• RefSeq ORF: 5214 bp
• Locus ID: 406161
• Cytogenetics: 20p12
• MW: 192.1 kDa