Nop56 (NM_001025732) Rat Tagged ORF Clone

CAT#: RR205032

  • TrueORF®

Nop56 (Myc-DDK-tagged ORF) - Rat nucleolar protein 5A (Nol5a), (10 ug)


  "NM_001025732" in other vectors (3)

Reconstitution Protocol

USD 790.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Nop56
Synonyms Nol5a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR205032 representing NM_001025732
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCTGCTGCACGTGCTCTTCGAGCATGCGGTCGGCTACGCGCTGTTAGCGCTGAAGGAAGTAGAGG
AGATCAGCTTGCTGTTGCCGCAGGTGGAGGAGTGTGTGCTTAACCTTGGCAAATTCCACAATGTCGTTCG
TCTGGTGGCCTTTTGCCCCTTTTCTTCATCCCAGGTTGCCTTGGAAAATGCTAATGCTGTGTCTGAAGGT
GTTGTTCATGAGGACCTCCGCCTGCTCCTGGAGACATACCTGCCATCTAAAAAGAAGAAAGTACTTCTGG
GGGTCGGAGACCCCAAGATCGGTGCTGCTATACAGGAAGAGTTGGGGTACAACTGCCAGACTGGTGGTGT
GATAGCTGAGATCCTTCGAGGGGTTCGTCTGCATTTCCATAACCTGGTGAAGGGTCTGACAGATCTTTCT
GCTTGTAAAGCCCAGCTAGGGCTGGGACACAGCTATTCTCGTGCCAAAGTTAAGTTTAATGTGAACCGGG
TGGACAACATGATCATTCAGTCTATAAGCCTTCTGGACCAACTGGATAAAGATATCAATACCTTCTCCAT
GCGTGTCAGGGAGTGGTATGGGTACCACTTTCCAGAGCTGGTGAAGATTATCAACGACAATGCTACATAC
TGTCGCCTGGCTCAGTTCATCGGGAACCGCAGGGAGCTAAATGAAGAAAAGTTAGAGAAGCTGGAAGAAA
TTACAATGGATGGAGCCAAGGCTAAGGCCATTCTGGATGCCTCTCGGTCCTCCATGGGCATGGACATATC
TGCTATCGACTTGATAAACATCGAGAGCTTCTCCAGTCGTGTGGTGTCTTTGTCAGAGTATCGCCAGAGC
CTACACAATTACCTTCGCTCCAAGATGAGCCAAGTAGCCCCCAGCCTGTCAGCCTTAATTGGGGAAGCGG
TAGGTGCACGTCTCATTGCCCATGCTGGCAGCCTCACCAACCTGGCCAAGTACCCAGCATCCACAGTGCA
GATCCTTGGGGCTGAAAAGGCCCTGTTCAGAGCCCTGAAGACAAGGGGTAACACACCAAAATACGGACTC
ATTTTTCATTCCACTTTCATTGGCCGAGCAGCTGCAAAGAACAAAGGCCGTATCTCCCGATACCTAGCAA
ACAAATGCAGTATTGCCTCAAGAATTGATTGCTTCTCTGAGGTCCCCACAAGTGTATTTGGGGAGAAACT
CCGGGAACAAGTTGAAGAGCGGCTTTCTTTCTATGAGACTGGAGAGATTCCACGGAAGAACCTGGATGTC
ATGAAAGAAGCTGTGGTACAGGCAGAGGAAGCGGCTGCTGAAATAACCAGAAAGCTGGAAAAACAGGAGA
AGAAACGCTTGAAGAAGGAAAAGAAGCGACTGGCTGCACTGGCCCTGGCCTCTTCAGAAAACAGTAGTAC
TCCGGAAGAGTGTGAGGAGGTAAATGAAAAACCTAAGAAGAAAAAGCTAAAACCCCAGGAAACCCTACAG
GAAAATGGAATGGAAGACCCACCTGTCTCTTTGCCTAAAACCAAGAAAAAGAAAGCTTTTCCCAAGGAGG
AGTTGGCCAGTGATCTTGAAGAGATGGCTACTAGCAGCATAAGTGTTCCTAAGAAAAAGAAGTCCTTACC
TAAAGAGGAAGTGGCCAGTGAACCCGAAGAGGCAGCGAGCCCCATCACCCCTAAGAAGAAAAGGAAATTT
TCTGAGGAGCCTGAGGCTGCTGCAAGCTGCACAAAGAGCAGCACAAAGAAAAAGAAAAAGTCGCAGAAGG
CCCGGGAGGAGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR205032 representing NM_001025732
Red=Cloning site Green=Tags(s)

MVLLHVLFEHAVGYALLALKEVEEISLLLPQVEECVLNLGKFHNVVRLVAFCPFSSSQVALENANAVSEG
VVHEDLRLLLETYLPSKKKKVLLGVGDPKIGAAIQEELGYNCQTGGVIAEILRGVRLHFHNLVKGLTDLS
ACKAQLGLGHSYSRAKVKFNVNRVDNMIIQSISLLDQLDKDINTFSMRVREWYGYHFPELVKIINDNATY
CRLAQFIGNRRELNEEKLEKLEEITMDGAKAKAILDASRSSMGMDISAIDLINIESFSSRVVSLSEYRQS
LHNYLRSKMSQVAPSLSALIGEAVGARLIAHAGSLTNLAKYPASTVQILGAEKALFRALKTRGNTPKYGL
IFHSTFIGRAAAKNKGRISRYLANKCSIASRIDCFSEVPTSVFGEKLREQVEERLSFYETGEIPRKNLDV
MKEAVVQAEEAAAEITRKLEKQEKKRLKKEKKRLAALALASSENSSTPEECEEVNEKPKKKKLKPQETLQ
ENGMEDPPVSLPKTKKKKAFPKEELASDLEEMATSSISVPKKKKSLPKEEVASEPEEAASPITPKKKRKF
SEEPEAAASCTKSSTKKKKKSQKAREED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001025732
ORF Size 1764 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001025732.1, NP_001020903.1
RefSeq Size 1936 bp
RefSeq ORF 1767 bp
Locus ID 362214
Cytogenetics 3q36
MW 65.4 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.