Hsd3b1 (NM_001007719) Rat Tagged ORF Clone

CAT#: RR205099

Hsd3b1 (Myc-DDK-tagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd3b1
• Synonyms: MGC105549
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205099 representing NM_001007719
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGATGGAGCTGCCTGGTGACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCCGGATGTTGG
  TGCAGGAGAAAGAACTGCAGGAGGTCAGGGCTCTGGACAAAGTCTTCAGACCAGAAACCAAGGAGGAATT
  CTCTAAGCTGCAGACAAAGGCCAAGGTGACAATGTTAGAAGGAGATATTCTGGATGCCCAGTACCTGAGG
  AGAGCGTGCCAGGGCATCTCTGTTGTCATCCACACCGCTGCTGTCATTGATGTCTCACATGTCCTACCCA
  GGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAACATATTGGAGGCCTGCGTCGAAGCCAGTGT
  GCCAGCCTTCATCTACTGCAGCACAGTTGACGTTGCAGGGCCCAACTCCTACAAGAAGATCATCCTGAAT
  GGCCATGAGGAAGAGCATCATGAAAGCACATGGTCAGATGCATACCCATACAGCAAAAGGATGGCCGAGA
  AGGCAGTGCTGGCAGCCAATGGGAGCATCCTGAAAAATGGTGGCACACTGCATACTTGTGCCTTAAGGCC
  CATGTACATTTATGGGGAGAGAAGTCCATTCCTTTCTGTCATGATACTTGCGGCCCTCAAAAATAAGGGT
  ATTCTGAATGTTACTGGCAAATTCTCCATAGCCAATCCAGTGTATGTAGGCAATGTGGCCTGGGCACATA
  TTCTGGCTGCCAGGGGCCTTCGAGACCCCAAGAAGTCACAAAATGTCCAAGGACAGTTCTACTACATCTC
  AGATGACACCCCTCACCAAAGCTATGATGATTTAAATTGCACCCTGAGCAAGGAATGGGGCCTCCGCCTT
  GATTCCAGCTGGAGCCTTCCTCTGCCCCTGCTCTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCC
  TGCTGCGTCCATTTTACAACTATAGGCCACCCTTTAACTGCCACTTGGTCACACTGTCAAATAGCAAGTT
  CACTTTCTCCTACAAGAAAGCTCAGAGAGATCTGGGCTATGTGCCACTTGTCAGCTGGGAGGAAGCCAAG
  CAGAAAACCTCGGAGTGGATCGGGACACTAGTGGAGCAGCACAGGGAGACACTGGACACAAAGTCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205099 representing NM_001007719
  Red=Cloning site Green=Tags(s)
  
  MPGWSCLVTGAGGFVGQRIIRMLVQEKELQEVRALDKVFRPETKEEFSKLQTKAKVTMLEGDILDAQYLR
  RACQGISVVIHTAAVIDVSHVLPRQTILDVNLKGTQNILEACVEASVPAFIYCSTVDVAGPNSYKKIILN
  GHEEEHHESTWSDAYPYSKRMAEKAVLAANGSILKNGGTLHTCALRPMYIYGERSPFLSVMILAALKNKG
  ILNVTGKFSIANPVYVGNVAWAHILAARGLRDPKKSQNVQGQFYYISDDTPHQSYDDLNCTLSKEWGLRL
  DSSWSLPLPLLYWLAFLLETVSFLLRPFYNYRPPFNCHLVTLSNSKFTFSYKKAQRDLGYVPLVSWEEAK
  QKTSEWIGTLVEQHRETLDTKSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007719
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007719.1, NM_001007719.3, NP_001007720.3
• RefSeq Size: 1640 bp
• RefSeq ORF: 1122 bp
• Locus ID: 360348
• Cytogenetics: 2q34
• MW: 42 kDa
• Gene Summary: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. [UniProtKB/Swiss-Prot Function]