Mavs (NM_001005556) Rat Tagged ORF Clone

CAT#: RR205124

Mavs (Myc-DDK-tagged ORF) - Rat virus-induced signaling adapter (Visa), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mavs
• Synonyms: Visa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205124 representing NM_001005556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATTTGCTGAGGAAAAGACCTATAAGTATATCCGCTACAACCACAGCAAGTTTTGCTGTGTTGATG
  TTCTGGAGATCCTGCCTTACCTGTCCTGCCTCACAACCAGTGACCAGGATCGATTACGGGCTTCCTACAA
  GCAACTAGGAAACCAGGGCACACTCTGGGAACTCTTCAATACGCTCCAGCGTCGGCCTGGCTGGGTAGAG
  GTCTTCATCCGGGCACTGAGGATCTGTGAGCTGCCTGGGCTGGCTGAGCAAGTGACTCGAGTTTATCAGA
  GCTACCTGCCTCCTGGGGCCTCACTCCACTCCCTAGACCCATTGCAGTCACCAAGAATTCCTACCACGGT
  TTCCGAACCTTCTGCATTTGCAGCAGGCCACACCATCCCTGACAGTGGCTTCCAAGATAAACCAGGTTAC
  CCTAAGCCTGTCCAGGACACCCAGCCACCAAAGTCCCCAGTAGAGAATTCGGAGGAACCCCCACAGGCCA
  ACTTTGGGGCCATTCCAAGGATGTCGGGTGACTCTTTGATATCCTCACCTAACCCACCGGCCCTCAGCCC
  TCAGCCCTCCAGAGAGCATCCCGAGCAAGAACCCGAACTGGGGGGCCCCTCCACAGCAAATGTTGACTCT
  GTTCCCATAGCAACCTATGGACCTGTGTCTCCAACTGTTTCCTTCCAGCCCCTTCCACGTATTGCCCCGA
  GGACAAACCTCTCGCCTGGGGTCACAGTATCAGCTCTATCTGCCAAGACCACTTTGTCCTCCTCTTCTAC
  TGGATCAGCTTTTGCAAAGGGAGCAGGTGACCAGGCCAAGGCTGCCACCTGTGTCAGCACTAAAGAGGGA
  GTACCTACCAATTCTGTGACTACCAGCTCAGTGCCTTCCATCAAACCAGTACCAGTAAATACCATGTCTT
  CCAAGTTGCCCATCAGTACAAAGTCCACTGCTGCAACGCCCTCTACTGTGCCCACTAATATAGCGCCATC
  AAAATTACCCATCAACTCAGTATATACTGGCATAGTGCCATCCAAAGTGACTGCTAGTGTGGCCAAAGCA
  AGTGCCAGCACAATGCCACCTGAGAGGAACAACAAGCAAGCCAAGGAGACCCTGGAGGCTCCAGCAACCG
  TAGTCACCACTGGAAGCAGCCTGACCAGACCAGATATCAGTTCCAGGAGCTTGCACTCTGGGCCAGAGTT
  AAGCAAGCCAGGGGTGCTGGTGTCCCAGGTGGACAACGAGCCATTCTCAGCCTGCTCTATGGACCTTGCC
  ATCAGCCCTAGCACCTCCTTGGGCTCAGAACCCAACCATGGTCCAGAGGAGAATGAGTATTCATCCTTTC
  GAATCCAGGTGGATAAAAGTCCCAGTGTTGATCTGTTAGGAAGCCCTGAGCCACTAGCCACCCAGCAGTC
  CCCAGAAGAGGAAGAGCCTTGTGCCAGTTCAGTGTCCTGGGCTAAGTGGCTGGGGGCCACCAGTGCACTC
  TTGGCTGCATTCCTGGCAGTGATGCTCTACCGTAGCAGGCACCTGGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205124 representing NM_001005556
  Red=Cloning site Green=Tags(s)
  
  MTFAEEKTYKYIRYNHSKFCCVDVLEILPYLSCLTTSDQDRLRASYKQLGNQGTLWELFNTLQRRPGWVE
  VFIRALRICELPGLAEQVTRVYQSYLPPGASLHSLDPLQSPRIPTTVSEPSAFAAGHTIPDSGFQDKPGY
  PKPVQDTQPPKSPVENSEEPPQANFGAIPRMSGDSLISSPNPPALSPQPSREHPEQEPELGGPSTANVDS
  VPIATYGPVSPTVSFQPLPRIAPRTNLSPGVTVSALSAKTTLSSSSTGSAFAKGAGDQAKAATCVSTKEG
  VPTNSVTTSSVPSIKPVPVNTMSSKLPISTKSTAATPSTVPTNIAPSKLPINSVYTGIVPSKVTASVAKA
  SASTMPPERNNKQAKETLEAPATVVTTGSSLTRPDISSRSLHSGPELSKPGVLVSQVDNEPFSACSMDLA
  ISPSTSLGSEPNHGPEENEYSSFRIQVDKSPSVDLLGSPEPLATQQSPEEEEPCASSVSWAKWLGATSAL
  LAAFLAVMLYRSRHLAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005556
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005556.1, NP_001005556.1
• RefSeq Size: 2965 bp
• RefSeq ORF: 1524 bp
• Locus ID: 311430
• Cytogenetics: 3q36
• MW: 53.8 kDa