Itgal (NM_001033998) Rat Tagged ORF Clone

CAT#: RR205165

Itgal (Myc-DDK-tagged ORF) - Rat integrin alpha L (Itgal), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgal
• Synonyms: LFA-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205165 representing NM_001033998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTTCCGGATCCCTGGCCCCAGACTTCTGCTATTGGGGCTCCAGCTGTTTGTGAATGCCTGGAGCT
  ACAACCTGGACACGCGGCATGCGCAGAGCTTCTTGACACAAGCTGGAAGACATTTTGGGTACCAGGTCTT
  GCAGTTTGGAGATGGGGTTGTCGTGGGAGCTCCAGGAGAGGGGAACAGCACAGGAAACCTCTATCACTGT
  CACCCAAGCAGCGGCTCCTGCCAGCCAGTCCACCTAGGTTCTAACCATACCTCCAAATACTTGGGAATGA
  CCATGGTGACAGATGCTGCCAAGGGAAGCCTTTTGGCCTGTGATCCTGGACTGTCTCGGACCTGTGATCA
  GAACCCCTACCTCAGTGGGCTGTGCTACTTATTTTCCCAGAGCCTGGGGAAACCTATATTACAAAATCGT
  CCTGCTTATCAGGAATGTATGAAGGGCAATGTCGACCTAGTGTTTCTGTTCGATGGCTCACAGAGCTTGG
  ATAAAAAGGACTTTGAAAAAATCGTGGACTTCATGAAGGATGTGATGAGGAAACTCAGCAACACTTCCTA
  CCAGTTTGCTGCTGTTCAGTTTTCCACAGAGTGCAAAACAGAATTTACTTTCTTGGACTACATCAAGCTA
  AACAAGAATCCTGATGTTCTGCTAGGCAACGTGACGCCCATGTTCTTGCTGACCAATACCTTCCGTGCCA
  TCAACTACGTGGTGACACACGTGTTCAAAGAAGAGTCTGGTGCCAGGCCAGATGCTACCAAGGTGCTTGT
  CATCATTACAGACGGGGAAGCAAGTGACAATGGCAACATCGATGCAGCCCAAGACATAACCCGCTATATC
  ATCGGGATTGGCAAGCATTTTTCAACCACACAAAAGCAGGAGAAACTCCACATATTTGCCTCAAAACCTG
  TAGAGGAATTTGTCAAGATTCTGGACACATTTGAGAAGCTGAAGGATCTGTTTACTGATCTGCAGAGGAA
  GATTTATGCCATTGAGGGCACAAGCAAACAGGACCTGGCATCCTTTAATATGGAACTCTCCTCCAGCGGG
  ATCAGCGCAGACCTCAGCAAGGGCCATGCGGTTGTTGGAGCCGTTGGGGCTAAGGATTGGGCTGGGGGCT
  TTCTGGACCTGCATGAAGACCTGGAGGGTGCCACATTTGTTGGGCAGGAACCGCTGACCTCAGATGAGAG
  AGGAGGTTATCTGGGTTACACTGTGGCCTGGCTGACCTCCCGGAGCTCTAGACCACTGCTGGCAGCAGGA
  GCTCCGCGGTACCAGCATGTGGGACAAGTGCTGCTTTTCCAAGCCCCAGAAGCTGGAGGACATTGGAACC
  AGACTCAGAAGATAGAAGGGACTCAGATCGGATCTTACTTTGGTGGGGAGCTATGTAGCGTTGACCTGCA
  CCAAGATGGCGAGACAGATCTGCTGCTGATTGGAGCGCCCCTCTTCTACGGGGAGCAGAGAGGAGGCCGA
  GTGTCCGTTTACCAGAGAAGACGGTCACTGTTCGAAATGGTCTCAGAGCTGCAGGGTGACCCAGGCTACC
  CGCTTGGTCGGTTTGGAGCTGCCATAAGTGCCCTGACAGACATCAATGGGGATGGGCTGACTGATGTGGC
  CGTGGGAGCCCCTCTGGAGGAGCAGGGGGCTGTGTATATCTTCAATGGGAAGCCTGGTGGGTTCAGTTCC
  CAGTCAAGTCAGCGTATACCAGGAACCCAGGTGTCCCCGGGAGTCCGGTGGTTTGGACGCTCCATCCATG
  GGGTGAAGGACCTTGGAGGGGACAGGCTGGCAGATGTGGTTGTAGGAGCTGAGGGCCAGGTGATTGTGCT
  GAGCTCGAGGCCGGTGGTGGATATTGTCACTGAGCTGTCCTTCTCCCCAGACGAAATCCCAGTGCATGAG
  GTGGAGTGCTCCTACTCAGCCAGTCAGGAGCAGAAGGAAGGCGTCAAGCTCAAGGTGTGCTTCCAGATCC
  GCCCCCTTACGTCTCAGTTTCAAGGTCGCCTGCTTGCCAACCTCAGCTACACCCTGCAGCTGGACGGCCA
  TCGGACGAGGAGCCGAGGTCTGTTCCCAGGAGGAAGCCGTGAGCTCGGCGGAAACACATTTGTCACCCCA
  GATAGGTCCTGCATGGACTTCCACTTCCACTTCCCAATCTGCATTCAAGACCTCATCTCCCCTATCAATG
  TCTCTCTGAATTTCTCTCTTTTGGAGGAAGAAGGATCACCAAGGGACCAAAAGGGGGGCAGGGACATGCA
  GCCCATCCTGAGACCTTCGATCCATGCAGTCACTAAGGAGATCCCCTTTGAGAAGAACTGTGGTGAGGAT
  AAGAAGTGTGAGGCAGACCTGGCCCTGTCACCTCCTGCCAGATCCGGAGTCCTGCGTCTGATGTCCTCTG
  CCAGCCTTGCTGTGGAGTGGACACTGAGGAACTTGGGAGAAGACGCTTACTGGGTGCGACTGGACCTGGA
  CTTCCCCCGGGGCCTCTCCTTCCGAAAAGTGGAGATGCTTCAGCCACACAGCCAAATACCTGTGAGCTGC
  GAGGAGCTCACCGAGGAGTCGAGTCTCCTGACTAAGACCCTGAAATGCAATGTGAGCTCTCCCATCTTCA
  AAGCAGGCAAACAGATGACCCTCCAGGTGATGTTTAACACGCTACTCAACAGTTCCTGGGGAGATTTTGT
  CGAGCTGAACGGCACTGTGCACTGTGAGAACGAGAACTCAAGCCTCGGGAAGGACAACTCAGCCACCACC
  CGCATTCCTGTCCTGTACCCTGTCAACATCCTTACTGAGGACCAGGAGAACTCCACACTCTATATCAGTT
  TCACCCCTAAAGGTCCCAAGACTCAACAAGTCCAGCATATCTACAAGGTGAGGATTCAGCCTTCTGCCTA
  TGACCACAACATGCCCGCACTGGAGGCCTTGGTTGAGGTGCCGCAGCCTCACAGCGAGGGCCCCATCGCA
  CACACATGGACTGTGCATACGGATCCCCTTGTCACTTGCCACTGTGAGGACCTGAAGAAGCCAACCAGGG
  AAGCTGAGCCCTGTCTGCCTGGAGTCCAGTTCCGCTGCCCAATTGTCTTCAGGCAAGAGACCTTCATCCG
  AGTGGCCGGGACTGTGGAACTCTCAGAGAAAATCAAGGCCTCCTCCACACTCAGCCTCTGCAGCTCGCTC
  TCAGTCTCCTTCAACAGCAGCAAGCATTTCCATTTGTATGGTAGCAAGGCCTCCAAGGCCCAGGTCCTCA
  TGAAGGTTGACATGATCTATGAGAAGGAGATGCTTCACCTGTACGTGCTCAGCGGCATTGGGGGGCTATT
  GCTACTGTCCCTGATTTTCCTAGCGCTCTACAAGGTTGGTTTCTTCAAACGGAAGCTGAAGGAGAAGATG
  GAGGCGGATGGAGGTGTTCCAAATGGAAGCCCTGGCGAAGACGCTGACCCTCTGGCAGTACCTGGGGAAG
  AGACCAAAGATATGGGCTGTCTAGAGCCCCTCCGTGAGAGTGACAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205165 representing NM_001033998
  Red=Cloning site Green=Tags(s)
  
  MSFRIPGPRLLLLGLQLFVNAWSYNLDTRHAQSFLTQAGRHFGYQVLQFGDGVVVGAPGEGNSTGNLYHC
  HPSSGSCQPVHLGSNHTSKYLGMTMVTDAAKGSLLACDPGLSRTCDQNPYLSGLCYLFSQSLGKPILQNR
  PAYQECMKGNVDLVFLFDGSQSLDKKDFEKIVDFMKDVMRKLSNTSYQFAAVQFSTECKTEFTFLDYIKL
  NKNPDVLLGNVTPMFLLTNTFRAINYVVTHVFKEESGARPDATKVLVIITDGEASDNGNIDAAQDITRYI
  IGIGKHFSTTQKQEKLHIFASKPVEEFVKILDTFEKLKDLFTDLQRKIYAIEGTSKQDLASFNMELSSSG
  ISADLSKGHAVVGAVGAKDWAGGFLDLHEDLEGATFVGQEPLTSDERGGYLGYTVAWLTSRSSRPLLAAG
  APRYQHVGQVLLFQAPEAGGHWNQTQKIEGTQIGSYFGGELCSVDLHQDGETDLLLIGAPLFYGEQRGGR
  VSVYQRRRSLFEMVSELQGDPGYPLGRFGAAISALTDINGDGLTDVAVGAPLEEQGAVYIFNGKPGGFSS
  QSSQRIPGTQVSPGVRWFGRSIHGVKDLGGDRLADVVVGAEGQVIVLSSRPVVDIVTELSFSPDEIPVHE
  VECSYSASQEQKEGVKLKVCFQIRPLTSQFQGRLLANLSYTLQLDGHRTRSRGLFPGGSRELGGNTFVTP
  DRSCMDFHFHFPICIQDLISPINVSLNFSLLEEEGSPRDQKGGRDMQPILRPSIHAVTKEIPFEKNCGED
  KKCEADLALSPPARSGVLRLMSSASLAVEWTLRNLGEDAYWVRLDLDFPRGLSFRKVEMLQPHSQIPVSC
  EELTEESSLLTKTLKCNVSSPIFKAGKQMTLQVMFNTLLNSSWGDFVELNGTVHCENENSSLGKDNSATT
  RIPVLYPVNILTEDQENSTLYISFTPKGPKTQQVQHIYKVRIQPSAYDHNMPALEALVEVPQPHSEGPIA
  HTWTVHTDPLVTCHCEDLKKPTREAEPCLPGVQFRCPIVFRQETFIRVAGTVELSEKIKASSTLSLCSSL
  SVSFNSSKHFHLYGSKASKAQVLMKVDMIYEKEMLHLYVLSGIGGLLLLSLIFLALYKVGFFKRKLKEKM
  EADGGVPNGSPGEDADPLAVPGEETKDMGCLEPLRESDKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033998
• ORF Size: 3480 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033998.1, NM_001033998.2, NP_001029170.1
• RefSeq Size: 4363 bp
• RefSeq ORF: 3483 bp
• Locus ID: 308995
• Cytogenetics: 1q37
• MW: 127.6 kDa
• Gene Summary: human homolog encodes the integrin alpha L chain which combines with beta 2 chain to form LFA-1 [RGD, Feb 2006]