Abcb8 (NM_001007796) Rat Tagged ORF Clone

CAT#: RR205188

Abcb8 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family B (MDR/TAP), member 8 (Abcb8), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcb8
• Synonyms: MGC93731
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205188 representing NM_001007796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGTGCACTTATTTCGGGTCGGAATTCGGGGTGGCCCAGTTCCAAGATGGTCGCTACAATCCCTGC
  GCTTCCAGACGTTCTCAGCCGCCAGGTCCTCAGATGACCAGTTCAGCTCATGCCTCCTGCGGGCTGTAGC
  CCAGCTCCGGTCACAACTCCGCGCTCACCTCCCTCGATCCCCACCAGCTTCCCACCGGAGTACCTCTGCC
  TGGTGTTGGGTTGGGGGGACTTTGGTGGTCCCCGCTGTGCTGTGGCAGCATCCCCGTTTCTGCCTTATAG
  CACTATGTGAGGCCAAAGGGTCCCCTCCTGCCCAGCCCACACGTGCCCGGGAGCTGCGCTTTAAGTGGAA
  GCTATTCTGGCATTTCCTTCACCCCCACCTGCTGGCCCTGGGGCTAGCCATTGTGCTGGCCTTGGGTGCG
  GCACTAGTGAATGTGCAGATCCCCTTGCTCCTCGGCCAACTGGTGGAGATCGTCGCCAAGTACACGAGGG
  AGCATGTGGGGAGTTTCGTGTCTGAGTCCCGTAGGCTCAGTATCCAGCTGCTCCTACTCTATGGTGTTCA
  GGGACTACTGACCTTTGGATACCTAGTGCTGCTGTCCCACATGGGTGAGCGCATGGCCATGGACATGCGG
  AAAGCCCTTTTCAGCTCCCTGCTCCGGCAAGACATTGCTTTCTTTGATGCCAAAAAGACAGGGCAGCTAG
  TGAGCCGCTTGACTACTGATGTGCAAGAGTTCAAGTCATCCTTCAAGCTTGTCATCTCCCAGGGACTGCG
  CAGCTCCACCCAGGTGATCGGTAGCCTGATGACCCTGTCTATTCTGTCCCCGCGCCTTACACTGATGCTG
  GCTGTTGTCACACCTGCCCTCATGGGAGTGGGCACCCTGATGGGCTCAGGCCTCCGAAAGCTTTCTCGAC
  AGTGTCAGGAGCAGATTGCCAGGGCGACAGGTGTAGCAGACGAGGCCCTTGGCAGTGTTCGGACTGTTCG
  GGCCTTCGCAATGGAGAAGAGGGAGGAGGAACGCTATCAAGCAGAACTGGAGTCATGCTGCTGTAAAGCA
  GAGGAGCTGGGCAGGGGCATCGCCTTGTTCCAGGGGCTCTCAAACATCGCTTTCAACTGTATGGTCTTGG
  GCACCCTGTTCATTGGAGGCTCCCTTGTGGCTGGACAGCAGCTGAAAGGGGGAGACCTCATGTCCTTCCT
  GGTGGCTTCCCAGACAGTACAGAGGTCTATGGCCAGCCTCTCTGTCCTGTTTGGTCAGGTGGTGCGTGGG
  CTGAGTGCAGGAGCCCGAGTCTTTGAGTACATGTCCCTGAGCCCCGTCATCCCCTTGACCGGGGGCTACT
  CTATCCCCAGCAAGGACCTTCGTGGTTCTATCACCTTCCAGAATGTCAGCTTCAGCTACCCCTGCCGACC
  TGGCTTCAATGTGCTCAAGAACTTCACCCTGAAGCTGCCCCCTGGCAAGATTGTGGCTCTCGTTGGCCAG
  TCTGGGGGAGGAAAGACCACAGTTGCTTCCCTGCTGGAACGCTTCTATGACCCCACAGCTGGCGTGGTGA
  CGCTGGATGGGCATGACCTGCGTACTCTCGACCCCTCCTGGCTCCGGGGCCAGGTCATAGGTTTCATCAG
  CCAGGAGCCAGTCCTGTTTGCAACAACCATCATGGAGAATATCCGATTTGGGAAGCTAGATGCTTCCGAT
  GAAGAGGTGTATACAGCTGCACGGAAAGCCAATGCCCACGAGTTCATCAGCAGCTTCCCTGATGGCTACA
  GCACTGTGGTTGGTGAACGGGGCACAACCTTGTCCGGTGGTCAGAAGCAGCGCCTAGCCATCGCACGTGC
  CCTCATCAAGAGGCCCACAGTGTTGATCCTGGATGAAGCCACCAGTGCGCTGGACGCAGAGTCTGAGAGG
  ATAGTACAGGAGGCACTGGACCGGGCCAGTGCTGGTCGCACCGTGTTGGTCATTGCCCACCGGCTCAGTA
  CTGTTCGTGCCGCCCACTCCATCATCGTCATGGCCAATGGCCAAGTCTGTGAGGCTGGGACCCACGAAGA
  ACTCCTTCAAAAAGGCGGGCTCTACGCAGAGCTTATCCGGAGACAAGCCCTGGATGCCTCACTGCCCTCA
  GCCCCACCTGCAGAGAAGCCTGAAGACCACAGAAGCTGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205188 representing NM_001007796
  Red=Cloning site Green=Tags(s)
  
  MLVHLFRVGIRGGPVPRWSLQSLRFQTFSAARSSDDQFSSCLLRAVAQLRSQLRAHLPRSPPASHRSTSA
  WCWVGGTLVVPAVLWQHPRFCLIALCEAKGSPPAQPTRARELRFKWKLFWHFLHPHLLALGLAIVLALGA
  ALVNVQIPLLLGQLVEIVAKYTREHVGSFVSESRRLSIQLLLLYGVQGLLTFGYLVLLSHMGERMAMDMR
  KALFSSLLRQDIAFFDAKKTGQLVSRLTTDVQEFKSSFKLVISQGLRSSTQVIGSLMTLSILSPRLTLML
  AVVTPALMGVGTLMGSGLRKLSRQCQEQIARATGVADEALGSVRTVRAFAMEKREEERYQAELESCCCKA
  EELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLKGGDLMSFLVASQTVQRSMASLSVLFGQVVRG
  LSAGARVFEYMSLSPVIPLTGGYSIPSKDLRGSITFQNVSFSYPCRPGFNVLKNFTLKLPPGKIVALVGQ
  SGGGKTTVASLLERFYDPTAGVVTLDGHDLRTLDPSWLRGQVIGFISQEPVLFATTIMENIRFGKLDASD
  EEVYTAARKANAHEFISSFPDGYSTVVGERGTTLSGGQKQRLAIARALIKRPTVLILDEATSALDAESER
  IVQEALDRASAGRTVLVIAHRLSTVRAAHSIIVMANGQVCEAGTHEELLQKGGLYAELIRRQALDASLPS
  APPAEKPEDHRSCQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007796
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007796.1, NP_001007797.1
• RefSeq Size: 2145 bp
• RefSeq ORF: 2145 bp
• Locus ID: 362302
• Cytogenetics: 4q11
• MW: 77.8 kDa