Enah (NM_001012150) Rat Tagged ORF Clone

CAT#: RR205233

Enah (Myc-DDK-tagged ORF) - Rat enabled homolog (Drosophila) (Enah), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Enah
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205233 representing NM_001012150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTAGAATTTGTATCTTACTCGATACCAAAGCTCAGAAGTAGCCAGAGCCTCAGAATCAGTGAAC
  AGAGTATCTGTCAGGCAAGAGCTGCTGTGATGGTTTATGATGATGCCAATAAGAAATGGGTGCCAGCTGG
  TGGCTCAACCGGGTTCAGCAGAGTACATATATATCACCATACAGGCAACAACACATTCAGAGTGGTGGGC
  AGAAAGATTCAAGACCATCAGGTTGTGATAAACTGTGCCATTCCTAAAGGACTGAAGTACAATCAAGCTA
  CACAGACTTTCCACCAATGGCGGGATGCTAGACAGGTGTATGGTCTCAACTTTGGCAGCAAAGAGGATGC
  CAATGTCTTCGCAAGTGCCATGATGCATGCCTTAGAAGTGTTAAATTCACAGGAAGCAGGGCCAACATTG
  CCTAGACAGAATTCACAGCTACCTGCTCAAGTTCAAAATGGCCCATCCCAAGAAGAGATGGAAATCCAGA
  GAAGGCAACTGCAAGAGCAGCAGCGACAGAAGGAGCTGGAGAGGGAGCGAATGGAGAGGGAAAGGTTGGA
  GAGAGAACGACTAGAACGAGAGAGGCTAGAGAGGGAGCGCCTGGAACAGGAGCAGCTGGAGCGGCAGAGG
  CAGGAGAGGGAGCACGTGGAGCGCCTGGAAAGGGAGCGCCTGGAGCGGCTGGAGAGAGAGAGGCAAGACC
  GAGAGCGAGAGCGCGAGCGCCTGGAACAGCTGGAGCGAGAGCAAGTGGAGTGGGAGAGAGAGCGCAGAAT
  GTCCAATGCTGGCCATGTCTTGGGACCACCTGCACCTCCACCACCACCCCCCCTACCATCAGGCCCTGCC
  TATGCTTCAGCACTTCCCCCTCCTCCTGGACCCCCTCCACCACCTCCACTGCCATCCGCTGGACCTCCTC
  CTCCACCGCCTCCACCACCCCCTCTTCCTAATCAAGTTCCTCCCCCTCCTCCACCACCTCCTGCCCCTCC
  CCTCCCCGCATCTGGAATTTTCTCTGGATCCATGTCAGAAGACAATCGCCCTTTAACTGGACTTGCAGCT
  GCAATCGCGGGAGCAAAACTTAGGAAAGTGTCTCGGGTGGAGGATGGCTCTTTCCCAAGTGGAGGAAATA
  CTGTGAGTGTGAACTTGGCCTCATCCAAAACAGATTCTGGTCGTGGCAATGGACCCCTTCCTCTAGGGGG
  CAGTGGCTTAATGGAAGAAATGAGTGCCCTGCTGGCCAGGAGGAGAAGAATTGCTGAGAAGGGATCAACA
  ATAGAAACAGAACAAAAAGAAGACAGAAGTGAAGATGCAGAGCCTGTAACTTCTAAGGCAGCATCAACAA
  GTACACCTGAACCAACCAGAAAACCTTGGGAAAGAACAAACACAATGAACGGCAGTAAGTCACCTGTCAT
  CTCCAGACCCAAATCCACACCTTCCTCACAGCCAAGTGCCAATGGGGTCCAGACAGAAGGGCTTGACTAT
  GACAGGCTGAAGCAGGACATTTTAGATGAAATGAGAAAAGAACTTGCAAAGCTGAAGGAGGAGCTCATTG
  ATGCAATCAGGCAGGAACTGAGCAAGTCGAATACTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205233 representing NM_001012150
  Red=Cloning site Green=Tags(s)
  
  MDVEFVSYSIPKLRSSQSLRISEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVG
  RKIQDHQVVINCAIPKGLKYNQATQTFHQWRDARQVYGLNFGSKEDANVFASAMMHALEVLNSQEAGPTL
  PRQNSQLPAQVQNGPSQEEMEIQRRQLQEQQRQKELERERMERERLERERLERERLERERLEQEQLERQR
  QEREHVERLERERLERLERERQDRERERERLEQLEREQVEWERERRMSNAGHVLGPPAPPPPPPLPSGPA
  YASALPPPPGPPPPPPLPSAGPPPPPPPPPPLPNQVPPPPPPPPAPPLPASGIFSGSMSEDNRPLTGLAA
  AIAGAKLRKVSRVEDGSFPSGGNTVSVNLASSKTDSGRGNGPLPLGGSGLMEEMSALLARRRRIAEKGST
  IETEQKEDRSEDAEPVTSKAASTSTPEPTRKPWERTNTMNGSKSPVISRPKSTPSSQPSANGVQTEGLDY
  DRLKQDILDEMRKELAKLKEELIDAIRQELSKSNTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012150
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012150.1, NP_001012150.1
• RefSeq Size: 2003 bp
• RefSeq ORF: 1581 bp
• Locus ID: 360891
• Cytogenetics: 13q26
• MW: 58.8 kDa