Dnaaf1 (NM_001014154) Rat Tagged ORF Clone

CAT#: RR205317

Dnaaf1 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 50 (Lrrc50), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnaaf1
• Synonyms: Lrrc50; RGD1310542
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205317 representing NM_001014154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCCGAAGCCTCAGAGCCGCCGGTGGACAGCGCGGCGGAGCCCAGTCTGGAGGAGTCCGCAGGCG
  ATCACGGCGACGCAGGCCCAGGGGTCCGCAAAGAAGAAATTAATGAGACCAAGGAAACATGTGTGGGTCC
  TTGTACAACATCCTGCCAAAGTCAGCAGCAACCGAGCGGTGACAATGGGTCAGATGGCCTCTTTACCCAC
  TCGAGAGATGACAGAGATGATCGGGGCCCCAGGATGACGAAGCAGTTCCTCCAAAAGCTCTGTAAGCAGC
  ACAAGCTGTACGTCACCCCGGCCCTGAACGACACCCTGTATTTGCACTTTAAGGGCTTTGACCGCATTGA
  GAACCTGGAGGAGTACACGGGCCTGCGCTGCCTCTGGCTGGAGTGCAACGGCATCCAGAGGATCGAGAAT
  TTGCAGGCCCAGAGCGAGCTGCGCTGCCTCTTCCTGCAAGTGAACCTGTTGCACAAGATCGAAAACCTGG
  AGCCGCTCCAGAAGCTGGACGCCCTCAACCTGAGCAACAACTACATCAAGACCATTGAGAACCTCTCCTG
  TCTGCCGGTCCTTAACACTCTGCAAATGGCCCACAACCGCCTGGAGACCGTGGCAGACATTGAGCACCTC
  AGGGAGTGTCTGCAACTCTGTGTCCTCGACCTCTCCCACAACTCGCTGAGTGACCCCGAGATCCTGAGCG
  TGCTTGAGACCATGCCCTGTCTGCGTGTGCTGAACCTGATGGGGAACCCAGTGACCAAACACATCCCTAA
  CTACCGCAGGACGGTCACTGTCCGACTGAAGCACTTGACTTATCTGGACGACAGGCCGGTTTTCCCCAAG
  GACAGGGCGTGTGCAGAGGCCTGGGCCCGAGGCGGGTATGCTGCCGAGAAGGAAGAGCGTCACCAGTGGG
  AGAGCAGGGAACACAAGAAGATAACAGACAGCCTGGAAGCCTTGGCCATGATCAAGCGGCGGGCCGAGGA
  ACGGAAGAAGGCAAGAGACAGAGGGGAGACACCTCTGCCAGAGAGTGAAAAGAGTATCCCCACTAGCCCA
  GAGGCTCAGGAAAAGCCTCCCAAGGGGGAAACCCAGCAGAAGATGGAGTCGTTTGTGAAAGAGAGCTTTG
  AGGCCAAAGATGAACTGTTTCCAGAGAAGCCAGGAGAAGGAGAGGAGCTGTCTGTGGTGGTGGGGAATAG
  AGCAGTGGAAGATGCAGACCTGTCCGGGAACCTGGCTCACACCCAGACACCTGTGGTGGTCACCCCTGAA
  GAGGTCACATCTCCAGTGGAAGCTACTGATGGGGCCAGAACGGAAGACACAGAGGCCATTGCCCTGGAGA
  CCAAGGAGAAGCTTTTCATCGATGACCTGCCTGACTTGGAAGATGTTGATGGTACAGATGTGTCTGTGGA
  AGACCAGACAAAGGACACAGGCATCCGGAAAATCCAGGCCATCTCCAGCTTGAGTGACGACAGTGACCTG
  GAGCTGGAGGAGCTGCCCCTCTCCGTGTTCGAGGGCACTCCCATAAGCCCCACAGGCGCCCTCTCACACA
  TATTTGCGGTCTCCAAAGACCCCTCAGAGGCAGCCAGGGTGCCTTTCGCAGACATATGTATGCCAACAGC
  AACAACAGACTTAGAAACTCAAAGTCAAGACCCCAGCACAGCATCTTCACACCCCCTCATCCAGGAGCTC
  GGAGAGGATGAGCTGACAGAGGGAGAGTCAAACCAGCCACTGCCACCCCAAAGCTGTGCAAGTGACCCCA
  CACTTGCCCAGTCTAGTGAAGGTGGAGACAGTCAGCTGCCTGCAGCCACTCCACTAGGAGATGGAGCTGA
  GAATGAAGCACAGTCTTCGTTGTACCCAGAGGAGCCCAGCACCCGGATAGGCCTGGAAGATATTGAATTT
  GGCTTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205317 representing NM_001014154
  Red=Cloning site Green=Tags(s)
  
  MHPEASEPPVDSAAEPSLEESAGDHGDAGPGVRKEEINETKETCVGPCTTSCQSQQQPSGDNGSDGLFTH
  SRDDRDDRGPRMTKQFLQKLCKQHKLYVTPALNDTLYLHFKGFDRIENLEEYTGLRCLWLECNGIQRIEN
  LQAQSELRCLFLQVNLLHKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNRLETVADIEHL
  RECLQLCVLDLSHNSLSDPEILSVLETMPCLRVLNLMGNPVTKHIPNYRRTVTVRLKHLTYLDDRPVFPK
  DRACAEAWARGGYAAEKEERHQWESREHKKITDSLEALAMIKRRAEERKKARDRGETPLPESEKSIPTSP
  EAQEKPPKGETQQKMESFVKESFEAKDELFPEKPGEGEELSVVVGNRAVEDADLSGNLAHTQTPVVVTPE
  EVTSPVEATDGARTEDTEAIALETKEKLFIDDLPDLEDVDGTDVSVEDQTKDTGIRKIQAISSLSDDSDL
  ELEELPLSVFEGTPISPTGALSHIFAVSKDPSEAARVPFADICMPTATTDLETQSQDPSTASSHPLIQEL
  GEDELTEGESNQPLPPQSCASDPTLAQSSEGGDSQLPAATPLGDGAENEAQSSLYPEEPSTRIGLEDIEF
  GLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014154
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014154.1, NP_001014176.1
• RefSeq Size: 2204 bp
• RefSeq ORF: 1902 bp
• Locus ID: 361419
• Cytogenetics: 19q12
• MW: 70 kDa