Rpap1 (NM_001033999) Rat Tagged ORF Clone

CAT#: RR205498

Rpap1 (Myc-DDK-tagged ORF) - Rat RNA polymerase II associated protein 1 (Rpap1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rpap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205498 representing NM_001033999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTGTCCAGACCGAAGCCTGGAGAGTCTGAGGTGGACCTACTGCGCTTCCAAAGTCAGTTCCTCG
  AGGCTGGTGCAGCTCCAGCGGTGCAGCTGGTGAAAGGGAGTAGGAGGCGTGGTGATGCGCATCCAGACCA
  GCTCCCCCCACAGGACCATCGGGATGTGGTGATGCTGGACAGTCTCCCAGATTTGCCCCCGGCTCTGCTT
  CCTGCTCCTTCCAAGAGAGCTAGACCAAGCCCTGGCCGCCCCCTGCCTCACGATGAAGACCCTGAGGAGA
  GGCTGAACAGACACGATGAGCACATCACTGCTGTTTTGTCTAAGATTGTTGAACGGGATACAAGTTCAGT
  TACTGTGACTCTGCCTGTGCCCAGTGGTGTGGCTTTCCCCCCTGTGTTCCACCGCTCTCAGGAGAGACAG
  GTGAAACCAGCAGCATCTAGTAAAAGGAGCATCTTTGCCCAAGAGATTGCAGCAAGGAGGGTGTCAGACA
  ACAGGGCACCATCAGCTGAGCAAGTTGTCCCCAGCCCAGATGCACCAGAGGGTGCCGTGCCCTGTGAAAC
  ACCCTCCTCTAAGGACAGAGGCAGCCAGCTTCCTGGGAGGAGCCATAGCTTCCACAGACCAAATCTAATC
  ACAGGAAAGGGGCTCAGGAGCCAGGCGGCTGTGCAGGAGGTCCAGACCATCCATGAAGAGAATGTAGCAA
  GACTACAAGCCATGGATCCTGAGGAGATCCTGAAGGAGCAGCAGCAGTTACTGGCTCAACTTGACCCCAG
  CTTGGTTGCCTTCTTGAGAGCTCATAACCATACCCGGGAGCAAACAGAAACAAAAGCCACCAAGGAGCAG
  AACCCAGAAAGGCCCTCAGTTCCTGTCAGTAAAGAAGAGCCCATCATGTCAACATGTACCGGGGAGTCTG
  GGACCAGGGACAAGCTGGAGGACAAGTTGGAGGACAAGCTGCAGCCAAGAACCCCAGCACTGAAGCTCCC
  CATGACCCCCAACAAAGAATGGCTCCATATGGACACCGTAGAGCTGGAGAAGCTCCACTGGACCCAGGAC
  CTGCCACCGCTCCGGCGGCAGCAGACACAGGAGAGAATGCAGGCTCGATTCAGCCTTCAGGGAGAGCTCC
  TGGAGCCTGATGTGGATCTGCCCACACATCTGGGCCTGCACCACCACGGAGAAGAGGCAGAGAGAGCAGG
  GTACTCTCTACAGGAGCTGTTCCACCTGACCCGTAGCCAGGTATCCCAGCAGAGAGCACTGGCATTGCAT
  GTGTTGTCCCACATCGTCGGCAGGGCCCAGGCTGGTGAGTTTGGGGACCGTCTAGTGGGCAGTGTCTTGC
  GCCTCCTCTTGGATGCTGGCTTCCTCTTCCTGCTGCGCTTCTCTCTGGATGACAGGATAGACAGCGTCAT
  TGCAGCAGCTGTCCGAGCTCTTCGTGCTCTGCTGGTTGCTCCTGGAGATGAGGAGCTCCTTGACAGCACC
  TTCTCTTGGTATCATGGAGCTTCAGTGTTCCCTATGATGCCCAGCCACGACGATAAAGAGGATGAAGATG
  AGGACGAAGAACTCACAAAAGAAAAGGTGAATCGAAAGACCCCTGAGGAAGGAAGCCGCCCTCCACCCGA
  CCTGGCCAGACATGATGTCATCAAGGGTCTTCTGGCTACCAACCTGCTGCCTCGGTTCCGCTATGTGCTG
  GAGGTGACCTGCCCAGGGCCCTCTGTGGTCCTTGACATCCTGGCTGTGCTTATACGCCTGGCCCGGCATT
  CCCTGGAGTCAGCCATGAGGGTCCTGGAGTGTCCTCGGCTGATGGAGACCATAGTTCGGGAGTTCTTGCC
  GACCAGTTGGTCCCCTATAGGGGTGGGGCCTGCTCCCAGTCTATATAAAGTGCCTTGTGCTGCTGCCATG
  AAACTACTCAGGGTCCTGGCCTCAGCTGGGAGGAATATCGCTGCCCGACTGCTAAGCAGCTTTGATGTCA
  GGAGCCGACTGTGCCGATTCATAGCTGAAGCACCACGTGACCTGGCCTTGCCATTCGAAGAAGCAGAGAT
  CCTGACAACTGAGGCCTTCCGTCTCTGGGCCGTGGCTGCCTCCTATGGCCAGGGTGGTGACCTTTACAGG
  GAGCTGTACCCAGTGCTCATGCGGGCCCTGCAGACACTGCCTCCAGAGCTCAGCACTCATCCCCTGCAGC
  CCCTGTCCATGCAGCGGATGGCCTCTCTGCTCACTCTGCTCACCCAGCTGACCCTGGCAGCTAGCACACA
  GCCTGAGGCCACCAGTGGCTCTGTCGAGTCCTGTGTGGTGGCCATCCCTTCCTCAATCACCTGGACACAT
  GTATCTGGACTCAAGCCACTGGTTGAGCCATGTCTAAAGCAGACCCTAAAGTTTCTGCGCAGACCTGATG
  TGTGGAATGCCCTGGGTCCGGTGCCTAGTGCCTGCCTTCTGTTCTTGGGTGCTTACTATCAGACCTGGAG
  CCAGCAGTCAGGCTTGTGCCCAGAGGATTGGCTTCAGGACATGGAACGCTTTTTGGATGAATTCTTGCTG
  CCACTGCTGAGCCAGCCTCCTTTGGGCAGGATGTGGGATTCCTTAAGGGACTGCTCCCCGCTCTGCAATC
  CGCTGTCCTGTGCTTCCACCCCTGAAGCTCTCCCCAGCCTCGTGTCACTAGGCTGTGCAGGAGGCTGTCC
  CCCTCTCAGTGTGGCTGGCTCAGCCTCACCCTTCCCATTCCTCACTGCCCTCCTCTCTCTTATCAACACT
  CTGGGCCAGATCCACAAGGGGCTTTGCAGACAGCTGGCTGTTGTGTTGACTGCCCCAGGACTCCAGAACT
  ACTTTCTCCAGTGTGTGGCTCCTGCACCTGCGCCACAGCTCACACCCTTCTCTGCCTGGGCCCTGCGCCA
  TGAGTACCACCTACAGTACCTGGTGCTTTCCCTAGCCCAGAAAGCGGCAACATCGCAGCCAGAGCCAGCT
  GCCAGCACTGCCCTCCATCATGTTATGGCCCTGGTCCTGCTGAGCCGGCTGCTGCCTGGAAGTGAATTCC
  TCGCCCACGAGCTGCTGCTGAGCTGTGTGTTCCGGCTGGGGTTCCTGCCGGAAAATGCTTCAGGTGGTCC
  AGAGGCAGCTGACTTCTCTGATGGGCTGTCCTTAGGGAACAGTGGGGACCCTCACTGTAGACGAGGGGCT
  CTCCTAGTTCAAGCTTGCCAGGATCTCCCCAGCATCCGCAGCTGCTACCTGGCCCATTGTTCACCAGCTC
  GAGCCAGTCTGCTGACCTCCCAAGCGTTGTACCGTGGAGAGCTGCCGAGAGTCTCAAGTCTGCTGCTGCC
  TGTACCTAAGGAGCCACTGCTGCCCACTGACTGGCCCTTCCAGCCGCTGATCCATCTCTACCACCGAGCT
  TCAGATACTCCCTCTGGGCTGCCTGCTGCTGACACTGTAGGCATCACCATGCGGGTCCTACAGTGGGTGC
  TAGTTCTGGAGAGCTGGCGCCCTGAGGCCCTCTGGGCTGTGCCCCCTGCTGCCCGCCTGGCACGTCTCAT
  GTGTGTGTACCTGGTGGATAGCGAGCTTTTTCGAGAGACTCCCATACAACGTCTAGTGGCTGCTCTTTTA
  GCTCGGCTCTGTCAGCCCCAAGTCTTGCCAAACCTCAAGCTGGACTGCCCCCTCCCTGGCCTGACCTCCT
  TTCCTGACCTCTATGCCAGCTTCTTAGACCACTTTGAGGCCGTCTCATTTGGAGACCACTTGTTTGGGGC
  TCTGGTCCTCCTTCCTCTGCAGCGCCGGTTCAGTGTCACCTTGCGCCTTGCCCTCTTTGGGGAGCATGTG
  GGAGTATTGCGAGCACTTGGCCTGCCTCTGGCTCAGCTGCCTGTGCCTCTGGAGTGCTACACAGAGCCTG
  CTGAAGACAGCCTGGCTCTCCTTCAGCTCTACTTCCGGGCCCTGGTTACTGGTGCACTCCATGCACGTTG
  GTGCCCCGTCCTCTACACTGTAGCTGTGGCTCATGTCAATAGTTTCGTCTTCTGCCAGGACCCAAAGAGC
  TCAGATGAGGTGAAGGCTGCCCGAAGGAGTATGCTGCAGAAGGTGTGGCTACTGGCAGACAAGGATCTGC
  GGCAGCATCTCCTTCACTATAAACTCCCCAACTCCAGCCTCCCAGAAGGCTTTGAACTGTATCCCCAGTT
  ACCTCGCCTGAGGCAGCAGTACCTTCAGACGCTGCCCACAGAGGTGCTCCAGAACGGAGGATTCAAGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205498 representing NM_001033999
  Red=Cloning site Green=Tags(s)
  
  MMLSRPKPGESEVDLLRFQSQFLEAGAAPAVQLVKGSRRRGDAHPDQLPPQDHRDVVMLDSLPDLPPALL
  PAPSKRARPSPGRPLPHDEDPEERLNRHDEHITAVLSKIVERDTSSVTVTLPVPSGVAFPPVFHRSQERQ
  VKPAASSKRSIFAQEIAARRVSDNRAPSAEQVVPSPDAPEGAVPCETPSSKDRGSQLPGRSHSFHRPNLI
  TGKGLRSQAAVQEVQTIHEENVARLQAMDPEEILKEQQQLLAQLDPSLVAFLRAHNHTREQTETKATKEQ
  NPERPSVPVSKEEPIMSTCTGESGTRDKLEDKLEDKLQPRTPALKLPMTPNKEWLHMDTVELEKLHWTQD
  LPPLRRQQTQERMQARFSLQGELLEPDVDLPTHLGLHHHGEEAERAGYSLQELFHLTRSQVSQQRALALH
  VLSHIVGRAQAGEFGDRLVGSVLRLLLDAGFLFLLRFSLDDRIDSVIAAAVRALRALLVAPGDEELLDST
  FSWYHGASVFPMMPSHDDKEDEDEDEELTKEKVNRKTPEEGSRPPPDLARHDVIKGLLATNLLPRFRYVL
  EVTCPGPSVVLDILAVLIRLARHSLESAMRVLECPRLMETIVREFLPTSWSPIGVGPAPSLYKVPCAAAM
  KLLRVLASAGRNIAARLLSSFDVRSRLCRFIAEAPRDLALPFEEAEILTTEAFRLWAVAASYGQGGDLYR
  ELYPVLMRALQTLPPELSTHPLQPLSMQRMASLLTLLTQLTLAASTQPEATSGSVESCVVAIPSSITWTH
  VSGLKPLVEPCLKQTLKFLRRPDVWNALGPVPSACLLFLGAYYQTWSQQSGLCPEDWLQDMERFLDEFLL
  PLLSQPPLGRMWDSLRDCSPLCNPLSCASTPEALPSLVSLGCAGGCPPLSVAGSASPFPFLTALLSLINT
  LGQIHKGLCRQLAVVLTAPGLQNYFLQCVAPAPAPQLTPFSAWALRHEYHLQYLVLSLAQKAATSQPEPA
  ASTALHHVMALVLLSRLLPGSEFLAHELLLSCVFRLGFLPENASGGPEAADFSDGLSLGNSGDPHCRRGA
  LLVQACQDLPSIRSCYLAHCSPARASLLTSQALYRGELPRVSSLLLPVPKEPLLPTDWPFQPLIHLYHRA
  SDTPSGLPAADTVGITMRVLQWVLVLESWRPEALWAVPPAARLARLMCVYLVDSELFRETPIQRLVAALL
  ARLCQPQVLPNLKLDCPLPGLTSFPDLYASFLDHFEAVSFGDHLFGALVLLPLQRRFSVTLRLALFGEHV
  GVLRALGLPLAQLPVPLECYTEPAEDSLALLQLYFRALVTGALHARWCPVLYTVAVAHVNSFVFCQDPKS
  SDEVKAARRSMLQKVWLLADKDLRQHLLHYKLPNSSLPEGFELYPQLPRLRQQYLQTLPTEVLQNGGFKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033999
• ORF Size: 4200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033999.1, NM_001033999.2, NP_001029171.1
• RefSeq Size: 4813 bp
• RefSeq ORF: 4203 bp
• Locus ID: 311338
• Cytogenetics: 3q35
• MW: 154.8 kDa