Inpp5j (NM_133562) Rat Tagged ORF Clone

CAT#: RR205562

Inpp5j (Myc-DDK-tagged ORF) - Rat inositol polyphosphate-5-phosphatase J (Inpp5j), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Inpp5j
• Synonyms: Inpp; Pib5pa; Pipp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205562 representing NM_133562
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCCAGAGCAGGAGTGGTAGTGCCAAGTCGGGGACCAGAACTGGACTGGGACCCTTGCCTGGGA
  CCCATGGGGCTTTGCAAACTGGGACACCCTCGAAGAAGGTAAATTCAAGTTTTCAGCTTCCAGCAAAGAA
  CACGGGCCCAACACCCTCAGAACCAAGATTGGCCCTGGCACCGGTGGGACCACGAGCAGCTGTGTCACCT
  CCTTCAGAGAGACCAAGACTGGCTTTGTCATCTCCCCGACCCATCCTAGCTCCACTGTCCACCGCTGGCG
  AACAGAAAAGACCTCCACCTCACCGCAGCTCCAAGCCGGCTCCCACGTCTGTGGGCCAACTGGTAGTATC
  TGCCGCTGCAGGACCAAAGCCTCCGCCAGTCGCCTCGGTCTCAATCCTGGCTCCGAAGTCTCTGGGGCAG
  CTAGTAATATCTGCCTCTGCTATGCCAAGGCCTACTCCGGCTCCTCTAGGGCCCATTCTATCACCAACTT
  CCAGGGATCAGAAGCAGTTATCACCCACCTCTGTGGGACCTAAGCCAGCATTGGCAACCTCAGGCCTAAG
  CCTGGCACTGGCCTCTCAGGAGCAGCCCCCACAGTCCCCCTCCAGTCCTTCCCCTGTGCCCAGTCCCGTT
  CTGTCACCCTCTCAGGAGAGTCACCTGGCTCCAGCAACTGTGACATCTACCCCAGCTTCCGAGAGACAGT
  TGCCAGCTAGACAGAAGGATACGGCAGTTCGCAGGCCCATTCCCCCTGCAGACGGGTGTCTCCATACCCC
  AGTGCAGGCAGCTGGTCTTGCCACCTCTCCTCCAAGGGCCCAAACTTCCTCAGATCCTCGGCTTTCCCCC
  TCCTTCCGCGCAAGACCAGAGGCTCCGCGCCACAGTCCCGAGGATCCCGTCTTGCCGCCGCCACCACAGA
  CCCTGCCCTTGGATGTGAGCTCAGGCCTTCCAGAGTCTGGCACCCGCTCCCCTGGACTTCTGTCCCCTAC
  CTTTCGGCCAGGAATCCCTTCAAACCAGACTGTGCCCCCACCTCTGCCCAAGCCACCTCGGTCTCCCAGC
  CGCTCCCCTAGTCGTTCTCCCAACCGCTCTCCCTGTGTGCCCCCAGCCCCTGAGGTGGCACTCCCAAGAC
  CTGTCACCCAGGGTGCAGGGCCTGGCAAGTGCCCAAGCCCTAACCTACAGACACAAGAAAGCCCAGTCGC
  CACCGCCACCTCACCAACGTCTTCTTGGTCAGCTCAGCCTACCTGCAAGAGCGACCCTGGATTCCGGATC
  ACTGTGGTTACATGGAATGTGGGCACTGCCATGCCTCCTGACGATGTCACATCTCTTCTACACCTGGGTG
  GTGGCCACGATAGCGACGGTGCAGACATGATTGCCATCGGGTTGCAAGAAGTCAATTCCATGATCAACAA
  GCGGCTCAAGGATGCTCTCTTTACCGACCAGTGGAGTGAGCTCTTCATGGATGCGCTGGGGCCTTTCAAC
  TTCGTGCTGGTGAGCACCGTGAGGATGCAGGGCGTCATCCTGCTGCTCTTCGCCAAGTACTACCACCTAC
  CCTTCCTACGGGATGTGCAGACGGACTGTACTCGGACTGGCCTGGGTGGCTACTGGGGTAACAAGGGCGG
  AGTAAGTGTGCGCTTAGCAGCCTTCGGGCACATGCTGTGCTTCCTAAACTGCCACTTGCCCGCACACATG
  GACAAGGCGGAGCAGCGCAAGGATAACTTTCAAACTATCCTTAGCCTCCAGCAATTCCAGGGACCTGGTG
  CACACGGCATCCTGGATCATGACCTTGTATTCTGGTTTGGGGACCTGAACTTCCGCATTGAGAGCTATGA
  CCTACACTTCGTCAAGTTCGCCATAGACAGCAACCAACTCCATCAGCTCTGGGAGAAGGACCAGCTCAAC
  ATGGCCAAGAATACTTGGCCCATCTTGAAAGGTTTCCAGGAAGGGCCCCTTAATTTTGCCCCCACCTTCA
  AGTTTGATGTGGGGACCAACAAATATGATACGAGTGCCAAGAAGCGGAAGCCAGCCTGGACAGATCGCAT
  TCTGTGGAAGGTCAAGGCTCCAAGTGGAGGCCCCAGCCCCTCAGGGCGAGAGAGTCACAGGCTCCAGGTG
  ACCCAGCACAGCTATCGCAGTCACATGGAATACACCGTCAGTGACCACAAGCCTGTTGCCGCCCGCTTCC
  TTCTGCAGTTTGCCTTCAGGGATGATGTACCTCTGGTACGGCTGGAGGTGGCGGACGAGTGGGCAAGGCC
  GGAGCAGGCTGTGGTGAGATACCGCGTGGAAACAGTATTTGCCCGAAGCTCCTGGGACTGGATTGGCTTG
  TACCGGGTGGGTTTCCGTCACTGTAAGGATTATGTGGCTTATGTCTGGGCCAAGCATGAAGAAGTGGATG
  GGAATATCTACCAGGTGACCTTCAGTGAGGAGTCACTGCCCAAGGGCCATGGCGACTTCATCCTGGGTTA
  CTATAGCCACCACCACAGCATCCTCATTGGTGTCACAGAACCCTTTCAGATCTCGCTGCCTACCTCAGAA
  TCAGCCAGCAGCAGCACAGACAGCTCAGGCACCAGCTCCGAGGGGGAGGATGACAGCACTCTGGAGCTGC
  TGGCACCCAAGTCCCGCAGCCCCAGCCCTGGCAAGTCCAAGAGACACCGTAGTCGCAGCCCGGGCCTAGC
  ACGCTTCCCCAGTCTCGCTCTCCGTCCCTCATCCCGCGAACGCCGTGGTGGCAGCCGTAGTCCCTCACCC
  CAGAGCCGCCAGCTGCCTCGGGTAGCCCCCGACAGGGGTCACAGTAGTGGCAGCCGAGGTAGTAGTGAGG
  AGGGGCCCTCTGGGCCGCCTGGACCCTGGGCCTTCCCACCAGCTGTGCCTCGAAGCCTGGGCCTGCTTCC
  AGCCTTGCGCCTGGAGACCGTAGACCCAGGTGGCGGAGGCTCCTGGGGACCTGACCAGGAGGCCCCTGAC
  CCTAATAGCCTGTCCCCCAGCCCGCAGGGCAGGCTGGGGCTAGAGGACGGGGGTTTGGGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205562 representing NM_133562
  Red=Cloning site Green=Tags(s)
  
  MEGQSRSGSAKSGTRTGLGPLPGTHGALQTGTPSKKVNSSFQLPAKNTGPTPSEPRLALAPVGPRAAVSP
  PSERPRLALSSPRPILAPLSTAGEQKRPPPHRSSKPAPTSVGQLVVSAAAGPKPPPVASVSILAPKSLGQ
  LVISASAMPRPTPAPLGPILSPTSRDQKQLSPTSVGPKPALATSGLSLALASQEQPPQSPSSPSPVPSPV
  LSPSQESHLAPATVTSTPASERQLPARQKDTAVRRPIPPADGCLHTPVQAAGLATSPPRAQTSSDPRLSP
  SFRARPEAPRHSPEDPVLPPPPQTLPLDVSSGLPESGTRSPGLLSPTFRPGIPSNQTVPPPLPKPPRSPS
  RSPSRSPNRSPCVPPAPEVALPRPVTQGAGPGKCPSPNLQTQESPVATATSPTSSWSAQPTCKSDPGFRI
  TVVTWNVGTAMPPDDVTSLLHLGGGHDSDGADMIAIGLQEVNSMINKRLKDALFTDQWSELFMDALGPFN
  FVLVSTVRMQGVILLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNKGGVSVRLAAFGHMLCFLNCHLPAHM
  DKAEQRKDNFQTILSLQQFQGPGAHGILDHDLVFWFGDLNFRIESYDLHFVKFAIDSNQLHQLWEKDQLN
  MAKNTWPILKGFQEGPLNFAPTFKFDVGTNKYDTSAKKRKPAWTDRILWKVKAPSGGPSPSGRESHRLQV
  TQHSYRSHMEYTVSDHKPVAARFLLQFAFRDDVPLVRLEVADEWARPEQAVVRYRVETVFARSSWDWIGL
  YRVGFRHCKDYVAYVWAKHEEVDGNIYQVTFSEESLPKGHGDFILGYYSHHHSILIGVTEPFQISLPTSE
  SASSSTDSSGTSSEGEDDSTLELLAPKSRSPSPGKSKRHRSRSPGLARFPSLALRPSSRERRGGSRSPSP
  QSRQLPRVAPDRGHSSGSRGSSEEGPSGPPGPWAFPPAVPRSLGLLPALRLETVDPGGGGSWGPDQEAPD
  PNSLSPSPQGRLGLEDGGLGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133562
• ORF Size: 3003 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133562.1, NP_598246.1
• RefSeq Size: 3322
• RefSeq ORF: 3006
• Locus ID: 171088
• MW: 107.2 kDa
• Gene Summary: hydrolyzes phosphate at the D-5 position of inositol or phosphatidylinositol polyphosphates; may mediate the level of inositol and phosphatidylinositol polyphosphates at membrane ruffles [RGD, Feb 2006]