Arhgef2 (NM_001012079) Rat Tagged ORF Clone

CAT#: RR205579

Arhgef2 (Myc-DDK-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef2
• Synonyms: MGC95068
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205579 representing NM_001012079
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGATCGAATCCCTCACTCGCGCGCGGATCGACCGGAGCAAGGAGCAGGCGACCAAGACCCGGG
  AAAAGGAGAAGATGAAGGAAGCTAAAGATGCCCGCTATACCAACGGCCACCTCTTCACCACCATCTCGGT
  CTCCGGCATGACTATGTGCTATGCCTGTAACAAGAGCATTACAGCCAAGGAAGCCCTCATTTGTCCCACA
  TGTAACGTGACCATCCACAACCGCTGTAAGGACACGCTGGCCAACTGTACCAAGGTCAAGCAGAAGCAAC
  AGAAAGCTGCACTGCTGAGGAACAACACTGCCTTGCAGTCCGTCTCTCTCCGAAGTAAGACAACCACCAG
  AGAGCGGCCAACGTCTGCCATCTACCCTTCCGATAGCTTCCGGCAGTCCCTCCTGGGTTCTCGTCGTGGC
  CTGTCCTCCTTGTCTTTGGCCAAAAGTGTTTCCACTACCAACATTGCTGGACATTTCAATGATGAGTCTC
  CTCTGGGGCTGCGTCAGATCCTCTCCCAGTCCACAGACTCCCTCAACATGCGGAACCGAACCCTGTCCGT
  GGAGTCCCTTATTGATGAAGGTGTAGAAGTGTTCTACAACGAGCTCATGAGTGACTTTGAGATGGACGAG
  AAGGACTTCGAGGCGGATTCATGGAGCCTTGCTGTGGACAGCAGCTTCCTGCAACAGCATAAAAAGGAGG
  TGATGAAGAAGCAAGATGTCATCTACGAGCTGATCCAGACCGAGCTGCACCATGTGAGAACCTTGAAGAT
  TATGACCCGCCTCTTCCGCACTGGGATGCTGGAAGAGTTGCAGATGGAGCCAGAAGTGGTCCAGGGACTG
  TTCCCCTGTGTGGACGAGCTTAGTGACATTCACACACGTTTCCTTAGTCAGCTTTTGGAACGGCGGCGCC
  AGGCCCTGTGTCCAGGCAGCACCCGGAACTTTGTCATCCATCGTTTGGGTGACTTGCTCATCAGCCAGTT
  CTCAGGTTCCAACGCTGAGCAGATGCGCAAGACTTACTCAGAGTTCTGCAGCCGCCACACCAAGGCCTTA
  AAGCTCTATAAGGAGCTGTACGCTCGAGACAAACGCTTCCAACAGTTCATCCGGAAAATGACCCGCTCTG
  CTGTGCTGAAGCGGCATGGGGTTCAGGAGTGCATTCTCCTGGTAACTCAGCGGATTACCAAATACCCAGT
  GCTCATCAACAGGATCCTGCAGAATTCCCATGGGATTGAAGAGGAGTACCAAGACTTGGCAGCAGCCCTA
  GGGCTAGTAAAGGAGTTGTTGTCCAATGTGGACCAGGATGTACACGAGCTGGAGAAAGAGGCCCGCCTTC
  AGGAGATTTACAACCGAATGGATCCTCGGGCTCAGACCCCTGTTCCTGGCAAGGGCCCCTTCGGCCGAGA
  TGAACTTTTGCGGAGAAAACTTATCCATGATGGCTGCCTGCTCTGGAAGACAGCCACAGGCCGCTTTAAA
  GATGTCCTCTTACTACTGATGACAGATGTGCTGGTGTTTCTCCAGGAAAAGGACCAGAAGTACATTTTCA
  CGTCCCTGGACAAGCCCTCAGTGGTATCCTTGCAGAATCTCATCGTAAGAGACATTGCCAACCAGGCGAA
  AGGGATGTTTCTGATCAGTTCTGGCCCACCTGAAATGTATGAAGTACACGCAGCATCTCGAGATGACCGT
  ACTACCTGGATCCGTGTCATCCAGCAGAGCGTGCGCTTATGCCCGTCGAGGGAGGACTTCCCTCTGATCG
  AGACAGAGGATAAGGCGTACCTCCGGAGGATCAAGACGAAACTGCAGCAGAAAAACCAGGCGCTGGTGGA
  GCTGCTACAGATGAATGTTGAGCTATTTGCTGAGATGGTCCACTTCCAGGCATTAAAAGCTGGTTTCATC
  GGAATGCCCCCGCCCACCCTGCCCAGGGGCCTTTTCCGTCTTGAGTCCTTTGAGTCCCTTCGAGGCGAGC
  GTCTGCTCAAAGATGCCCTCCGTGAAGTGGAAGGCCTGAAAGACCTGTTGTTGGGCCCTTGTGTGGACCT
  GCCTTTGACAGCCCGAGAACCAGCCTTACCCGTAGAAGCTGACAGTGGTAGCTGTCCTGGGGTCACTGCC
  AACGGAGAGGCCAGAACCTTCAATGGCTCCATTGAGCTCTGTAGAGCAGACTCAGATTCCAGCCAGAAGG
  ATCGGAATGGAAATCAGTTGAGGTCACCACAGGAGGAGGCGTTACAACCATTGGTCAATCTGTATGGACT
  CCTACAAGGCCTGCAGGCTGTTGTGGTCCAGCAAGAAAGATTGATGGAAGCCCTGTTCCCTGAAGGCCCT
  GAGCGGTGGGAAAAGCTATCCCGAGCCAACTCTCGGGATGGCGAAGCTGGCCGGGCTGCGGTTGCTTCTG
  TAACTCCCGAGAAGCAGGCCACGGAGCTGGCATTACTGCAGAGGCAGCACAGCCTGTTGCAGGAAGAGCT
  GCGGCGCTGCCAGCGGCTCGGGGAAGAGCGGGCAACTGAAGCTGGCAGCCTGGAGGCCAGGCTGCGGGAG
  AGCGAGCAAGCCCGGGCCCTGCTGGAGCGGGAGGCTGAAGAGATCCGCCGGCAGCTAGCAGCCTTGGGCC
  AAAACGAGCCACTCCCGGCAGAAGCACCCTGGGCTCGCAGGCCTCTGGACCCTCGGCGCCGCAGCCTTCC
  AGCAGGCGACGCTCTGTACTTGAGCTTCAATCCCCCCCAGCCTAGTCGAGGCCATGACCGCCTGGATTTG
  CCTGTGACTGTTCGTTCCCTCCACCGACCCTTTGATGACCGAGAGGCACAAGAACTTGGTAGCCCCGAGG
  ATCGACTGCAGGACAGCAGTGACCCTGATACTTGCAGTGAGGAGGAAGTCAGTAGCCGGCTGTCCCCACC
  CCACAGTCCACGAGACTTCACCCGAATGCAGGACATTCCCGAAGAGACAGAAAGCCGAGATGGGGAGCCT
  ACGGCCTCAGAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205579 representing NM_001012079
  Red=Cloning site Green=Tags(s)
  
  MSRIESLTRARIDRSKEQATKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPT
  CNVTIHNRCKDTLANCTKVKQKQQKAALLRNNTALQSVSLRSKTTTRERPTSAIYPSDSFRQSLLGSRRG
  LSSLSLAKSVSTTNIAGHFNDESPLGLRQILSQSTDSLNMRNRTLSVESLIDEGVEVFYNELMSDFEMDE
  KDFEADSWSLAVDSSFLQQHKKEVMKKQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQMEPEVVQGL
  FPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGSNAEQMRKTYSEFCSRHTKAL
  KLYKELYARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLINRILQNSHGIEEEYQDLAAAL
  GLVKELLSNVDQDVHELEKEARLQEIYNRMDPRAQTPVPGKGPFGRDELLRRKLIHDGCLLWKTATGRFK
  DVLLLLMTDVLVFLQEKDQKYIFTSLDKPSVVSLQNLIVRDIANQAKGMFLISSGPPEMYEVHAASRDDR
  TTWIRVIQQSVRLCPSREDFPLIETEDKAYLRRIKTKLQQKNQALVELLQMNVELFAEMVHFQALKAGFI
  GMPPPTLPRGLFRLESFESLRGERLLKDALREVEGLKDLLLGPCVDLPLTAREPALPVEADSGSCPGVTA
  NGEARTFNGSIELCRADSDSSQKDRNGNQLRSPQEEALQPLVNLYGLLQGLQAVVVQQERLMEALFPEGP
  ERWEKLSRANSRDGEAGRAAVASVTPEKQATELALLQRQHSLLQEELRRCQRLGEERATEAGSLEARLRE
  SEQARALLEREAEEIRRQLAALGQNEPLPAEAPWARRPLDPRRRSLPAGDALYLSFNPPQPSRGHDRLDL
  PVTVRSLHRPFDDREAQELGSPEDRLQDSSDPDTCSEEEVSSRLSPPHSPRDFTRMQDIPEETESRDGEP
  TASES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012079
• ORF Size: 2955 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012079.1, NP_001012079.1
• RefSeq Size: 4217 bp
• RefSeq ORF: 2958 bp
• Locus ID: 310635
• Cytogenetics: 2q34
• MW: 111.9 kDa