Chrm5 (NM_017362) Rat Tagged ORF Clone

CAT#: RR205682

Chrm5 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, muscarinic 5 (Chrm5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrm5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205682 representing NM_017362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGGAGTCTTACAATGAAAGCACTGTCAACGGCACCCCGGTAAATCACCAGGCTTTGGAACGCC
  ATGGACTGTGGGAAGTCATTACTATTGCAGTTGTGACTGCGGTGGTCAGCCTGATGACCATTGTCGGCAA
  TGTCTTGGTCATGATCTCCTTCAAAGTCAACAGTCAGCTCAAGACAGTTAACAACTACTACCTGCTCAGC
  TTGGCCTGTGCAGACCTCATCATTGGCATCTTCTCCATGAACCTCTACACGACCTACATCCTCATGGGAC
  GCTGGGTTCTCGGGAGTCTGGCTTGTGACCTTTGGCTGGCACTCGACTATGTAGCCAGCAATGCTTCTGT
  CATGAACCTTCTGGTGATTAGTTTTGATCGTTACTTTTCCATCACAAGACCACTGACGTACCGGGCCAAG
  CGTACCCCAAAGAGGGCTGGCATCATGATTGGCTTAGCATGGCTGGTCTCCTTCATCCTCTGGGCGCCAG
  CCATCCTCTGCTGGCAGTACTTGGTCGGGAAGCGGACAGTACCACCTGATGAGTGCCAGATCCAGTTCCT
  CTCTGAACCCACCATCACTTTTGGGACTGCCATTGCTGCCTTCTACATCCCTGTCTCCGTCATGACCATA
  CTCTACTGCCGGATCTACCGGGAGACAGAGAAGCGAACCAAGGACCTGGCTGACCTCCAAGGTTCTGATT
  CTGTGGCAGAAGCCAAGAAGAGAGAGCCAGCTCAAAGGACCCTGCTCAGATCTTTCTTTAGCTGCCCTAG
  ACCCAGCCTGGCCCAGAGAGAAAGGAATCAGGCCTCCTGGTCATCCTCCCGTAGAAGCACCTCAACAACA
  GGAAAGACAACCCAGGCCACTGACCTAAGTGCTGACTGGGAAAAGGCTGAGCAGGTTACTACCTGTAGCA
  GCTACCCCTCTTCAGAGGATGAAGCCAAGCCCACCACTGACCCTGTCTTTCAAATGGTCTACAAGAGTGA
  GGCCAAGGAAAGCCCGGGGAAGGAATCCAATACCCAAGAGACCAAGGAAACAGTTGTGAACACCCGGACT
  GAAAACAGTGACTATGACACTCCCAAATACTTTCTGTCTCCAGCTGCTGCTCACAGACTCAAGAGTCAGA
  AGTGTGTTGCCTATAAGTTCCGATTGGTGGTAAAAGCCGATGGGACCCAGGAGACTAACAATGGCTGTCG
  AAAGGTGAAAATCATGCCCTGTTCCTTCCCAGTGTCCAAAGACCCTTCAACAAAAGGTCCGGATCCCAAC
  CTCAGTCATCAAATGACCAAACGAAAGAGAATGGTTCTGGTCAAAGAGAGGAAAGCGGCTCAGACCTTGA
  GTGCCATTCTCCTGGCCTTCATCATCACATGGACCCCTTATAACATCATGGTCCTGGTTTCCACCTTCTG
  TGACAAGTGTGTCCCCGTCACCCTGTGGCACTTGGGTTACTGGCTGTGCTATGTCAACAGCACCATCAAC
  CCCATCTGCTATGCTCTCTGCAACAGAACTTTCAGGAAAACCTTTAAGCTGCTTCTTCTCTGCCGGTGGA
  AAAAGAAAAAAGTAGAAGAGAAATTGTATTGGCAAGGAAACAGCAAGCTACCCAAAAGAAAAAAGTAGAA
  GAGAAATTGTATTGGCAAGGAAACAGCAAGCTACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205682 representing NM_017362
  Red=Cloning site Green=Tags(s)
  
  MEGESYNESTVNGTPVNHQALERHGLWEVITIAVVTAVVSLMTIVGNVLVMISFKVNSQLKTVNNYYLLS
  LACADLIIGIFSMNLYTTYILMGRWVLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAK
  RTPKRAGIMIGLAWLVSFILWAPAILCWQYLVGKRTVPPDECQIQFLSEPTITFGTAIAAFYIPVSVMTI
  LYCRIYRETEKRTKDLADLQGSDSVAEAKKREPAQRTLLRSFFSCPRPSLAQRERNQASWSSSRRSTSTT
  GKTTQATDLSADWEKAEQVTTCSSYPSSEDEAKPTTDPVFQMVYKSEAKESPGKESNTQETKETVVNTRT
  ENSDYDTPKYFLSPAAAHRLKSQKCVAYKFRLVVKADGTQETNNGCRKVKIMPCSFPVSKDPSTKGPDPN
  LSHQMTKRKRMVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTIN
  PICYALCNRTFRKTFKLLLLCRWKKKKVEEKLYWQGNSKLPKRKK*KRNCIGKETASY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017362
• ORF Size: 1593 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 2733
• RefSeq ORF: 1596
• Locus ID: 53949
• MW: 60.1 kDa
• Gene Summary: M5 muscarinic receptor; stimulates phosphoinositide hydrolysis without affecting adenylyl cyclase activity [RGD, Feb 2006]