Rock1 (NM_031098) Rat Tagged ORF Clone

CAT#: RR205715

Rock1 (Myc-DDK-tagged ORF) - Rat Rho-associated coiled-coil containing protein kinase 1 (Rock1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rock1
• Synonyms: P160Rock; ROCK-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205715 representing NM_031098
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGACTGGGGACAGTTTTGAGACTCGGTTTGAAAAAATCGACAACCTGCTGCGGGATCCCAAATCGG
  AAGTGAATTCAGATTGTTTGCTGGATGGATTGGATGCTTTGGTTTATGATTTGGATTTTCCTGCCTTAAG
  AAAAAACAAAAATATTGACAACTTTCTAAGCAGATATAAAGACACAATAAATAAAATTAGAGATTTACGG
  ATGAAAGCTGAAGATTATGAAGTAGTAAAGGTAATCGGCAGAGGTGCATTTGGAGAAGTTCAATTGGTAA
  GGCATAAATCCACCAGGAAGGTTTATGCTATGAAGCTTCTCAGCAAATTTGAAATGATTAAGAGATCTGA
  TTCTGCTTTTTTCTGGGAAGAAAGGGACATCATGGCATTTGCCAATAGTCCTTGGGTTGTTCAGCTTTTT
  TATGCATTCCAAGATGACCGATATCTCTATATGGTGATGGAGTACATGCCTGGTGGAGACCTTGTAAACC
  TGATGAGCAACTACGATGTGCCTGAAAAGTGGGCACGATTCTATACTGCAGAAGTAGTTCTCGCATTGGA
  TGCRATCCATTCCATGGGTTTTATTCATAGAGATGTGAAGCCTGACAACATGCTGCTGGATAAGTCTGGA
  CATTTGAAGTTAGCCGACTTTGGTACTTGTATGAAGATGAATAAGGAAGGTATGGTACGATGTGATACAG
  CAGTTGGAACACCTGATTATATTTCACCTGAAGTATTAAAATCACAAGGCGGTGATGGCTATTATGGACG
  AGAGTGTGACTGGTGGTCAGTTGGTGTATTTTTATATGAAATGCTTGTAGGTGATACACCTTTTTATGCA
  GATTCTTTGGTTGGGACGTACAGTAAAATTATGAACCATAAAAATTCACTTACTTTCCCTGATGATAATG
  ACATATCAAAAGAGGCGAAAAATCTCATTTGTGCCTTCCTTACGGACAGAGAAGTGAGATTGGGCAGAAA
  TGGTGTAGAGGAAATCAAACGTCATCTCTTCTTCAAAAATGATCAGTGGGCTTGGGAAACGCTCCGAGAC
  ACTGTAGCACCAGTTGTGCCTGATTTAAGTAGTGACATTGATACTAGTAATTTTGATGACTTAGAAGAAG
  ATAAAGGAGATGAAGAAACATTTCCTATTCCAAAAGCTTTTGTTGGCAATCAGCTACCTTTTGTAGGATT
  TACATATTATAGCAATCGTAGATACTTACCTTCAGCAAATCCCAGTGAGAACAGATCTAGCTCCAATGTG
  GATAAAAACGTGCAGGAAAGTTTACAGAAAACAATCTACAAGCTTGAAGAACAACTGCATAATGAAATGC
  AATTAAAAGATGAAATGGAGCAAAAGTGCAGAACTTCAAACATAAAACTAGACAAGATAATGAAAGAATT
  GGATGAAGAGGGGAATCAAAGAAGAAATCTAGAATCTGCGGTGTCTCAAATTGAGAAAGAAAAGATGTTG
  CTACAACATAGAATTAATGAGTACCAAAGAAAAGTGGAACAGGAAAATGAGAAAAGAAGAAATGTAGAAA
  ATGAAGTTTCTACACTAAAGGACCAGTTGGAAGACTTACGGAAAGCAAGTCAGAGCTCACAGCTTGCTAA
  TGAGAAGCTGACACAGTTACAGAAGCAGTTAGAAGAAGCGAATGACTTACTTAGGACAGAATCAGACACA
  GCTGTAAGATTAAGGAAGAGTCACACAGAGATGAGCAAGTCAGTTAGTCAGCTGGAGTCTCTGAACAGAG
  AGTTACAGGAGAGAAACAGAATGTTAGAGAATTCCAAGTCACAAGCAGACAAGGATTACTACCAGCTGCA
  AGCTGTATTGGAAGCTGAGCGAAGAGACAGAGGCCATGATTCTGAGATGATTGGAGACCTTCAAGCACGA
  ATTACGTCTTTACAAGAAGAGGTGAAGCATCTCAAACATAATCTTGAAAGAGTAGAAGGAGAAAGAAAAG
  AGGCTCAAGACATGCTCAATCATTCAGAAAAGGAAAAAAATAATTTGGAGATTGATTTGAACTACAAGCT
  CAAGTCTATTCAGCAGAGGTTAGAACAAGAAGTAAATGAACACAAAGTAACCAAAGCGCGGCTAACTGAC
  AAGCATCAGTCTATTGAAGAGGCAAAGTCTGTTGCCATGTGTGAAATGGAAAAAAAGCTGAAGGAAGAGA
  GAGAAGCTCGAGAGAAGGCTGAAAATAGAGTTGTTGAGACTGAGAAGCAATGTTCTATGCTAGATGTTGA
  CCTGAAACAGTCTCAGCAGAAGCTAGAACATTTGACCGAAAATAAAGAAAGGCTGGAGGATGCAGTTAAG
  AGTCTCACCCTGCAGCTGGAACAGGAATCCAATAAGCGGATCTTGCTACAGAGTGAACTGAAGACTCAAG
  CGTTTGAAGCAGACAATTTAAAAGGGTTAGAAAAACAGATGAAACAGGAAATAAATACTTTATTGGAAGC
  TAAAAGATTGTTAGAATTTGAGTTAGCTCAGCTTACAAAGCAATACAGAGGAAATGAAGGGCAAATGCGG
  GAGTTACAAGATCAGCTTGAAGCTGAGCAATATTTCTCTACACTTTATAAAACCCAGGTAAAGGAACTTA
  AAGAAGAAATTGAAGAAAAAAACAGAGAAAATTTAAGGAAAATACAGGAGCTGCAAAGTGAAAAAGAAAC
  TCTTTCGACTCAGTTGGATTTAGCAGAAACAAAAGCTGAGTCTGAGCAGTTGGCTCGAGGAATTCTGGAA
  GAACAGTATTTTGAGTTGACTCAAGAAAGCAAGAAAGCTGCTTCAAGAAATAGACAAGAGATTACAGATA
  AAGATCATACCGTTAGTCGGCTTGAAGAAGCAAACAATGCGCTAACCAAAGATATTGAACTACTAAGAAA
  AGAAAATGAAGAGCTAAATGAAAGGATGAGAACAGCAGAAGAAGAATATAAATTGAAGAAGGAGGAAGAA
  ATAAGTAATCTTAAGGCTGCCTTTGAAAAGAATATCAGTACCGAACGGACCCTTAAAACACAGGCTGTTA
  ACAAGTTGGCAGAAATAATGAATCGCAAAGATTTTAAAATTGATAGGAAGAAAGCTAATACTCAAGATTT
  GAGAAAAAAAGAAAAGGAAAATAGAAAGCTCCAACTAGAACTTAACCAAGAAAGAGAAAAATTTAACCAA
  ATGGTGGTGAAACACCAGAAGGAGCTGAATGACATGCAAGCGCAATTGGTAGAAGAATGTACACATAGAA
  ATGAACTTCAAATGCAGTTGGCTAGCAAAGAGAGTGATATTGAGCAGTTGCGTGCAAAACTTTTGGACCT
  TTCGGATTCAACTAGTGTTGCTAGTTTTCCCAGTGCTGATGAGACTGACGGAAATCTTCCAGTTGGTTCT
  GCCTGCATTCCTTATTTATTTATCTTCTACTCTTCTAGTTCAAGAATTGAAGGTTGGCTTTCTGTACCAA
  ATAGAGGAAATATCAAAAGATATGGCTGGAAGAAACAGTATGTTGTGGTGAGCAGCAAAAAAATGTTGTT
  CTATAATGATGAGCAAGATAAGGAACAATCCAGTCCATCTATGGTGTTGGACATAGATAAATTGTTCCAT
  GTCCGACCTGTAACCCAAGGAGATGTGTACAGAGCTGAAACTGAAGAAATTCCTAAAATATTCCAGATAC
  TGTATGCAAATGAAGGTGAATGTAGAAAAGATATAGAAGTGGAACCAGTTCAACAAGGTGAAAAAACTAA
  TTTCCAGAATCACAAAGGCCATGAATTCATTCCTACCCTCTACCACTTTCCAGCCAACTGCGAGGCCTGT
  GCCAAACCTTTGTGGCATGTTTTTAAGCCACCGCCTGCCCTAGAGTGTCGAAGATGCCATGTTAAGTCCC
  ACAGGGACCATCTAGACAAGAAAGAGGACTTGATTCCCCCATGCAAAGTAAGTTATGATGTAACATCAGC
  AAGAGATATGTTGCTGTTAGCATGTCCTCAGGATGAACAAAAGAAATGGGTAACTCATCTGGTAAAGAAG
  ATTCCTAAGAAGGCACCTTCTGGCTTTGTCCGTGCCTCTCCTCGAACGCTGTCGACAAGATCTACTGCAA
  ATCAGTCTTTCCGGAAAGTAGTCAAAAATACATCTGGAAAAACTAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205715 representing NM_031098
  Red=Cloning site Green=Tags(s)
  
  MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLR
  MKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLF
  YAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDXIHSMGFIHRDVKPDNMLLDKSG
  HLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYA
  DSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRD
  TVAPVVPDLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLPSANPSENRSSSNV
  DKNVQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESAVSQIEKEKML
  LQHRINEYQRKVEQENEKRRNVENEVSTLKDQLEDLRKASQSSQLANEKLTQLQKQLEEANDLLRTESDT
  AVRLRKSHTEMSKSVSQLESLNRELQERNRMLENSKSQADKDYYQLQAVLEAERRDRGHDSEMIGDLQAR
  ITSLQEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSIQQRLEQEVNEHKVTKARLTD
  KHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVETEKQCSMLDVDLKQSQQKLEHLTENKERLEDAVK
  SLTLQLEQESNKRILLQSELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMR
  ELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLRKIQELQSEKETLSTQLDLAETKAESEQLARGILE
  EQYFELTQESKKAASRNRQEITDKDHTVSRLEEANNALTKDIELLRKENEELNERMRTAEEEYKLKKEEE
  ISNLKAAFEKNISTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQ
  MVVKHQKELNDMQAQLVEECTHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPVGS
  ACIPYLFIFYSSSSRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKMLFYNDEQDKEQSSPSMVLDIDKLFH
  VRPVTQGDVYRAETEEIPKIFQILYANEGECRKDIEVEPVQQGEKTNFQNHKGHEFIPTLYHFPANCEAC
  AKPLWHVFKPPPALECRRCHVKSHRDHLDKKEDLIPPCKVSYDVTSARDMLLLACPQDEQKKWVTHLVKK
  IPKKAPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031098
• ORF Size: 4107 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031098.1, NP_112360.1
• RefSeq Size: 4501
• RefSeq ORF: 4110
• Locus ID: 81762
• MW: 159.7 kDa
• Gene Summary: protein serine/threonine kinase; involved in intracellular signaling [RGD, Feb 2006]