Ddah1 (NM_022297) Rat Tagged ORF Clone

CAT#: RR205721

Ddah1 (Myc-DDK-tagged ORF) - Rat dimethylarginine dimethylaminohydrolase 1 (Ddah1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddah1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205721 representing NM_022297
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCCTCAGCCACCCCTCGGTCTTCGGCCGGGCCACCCACGCCGTGGTGCGGGCTCCGCCCGAGT
  CCCTGTGCCGCCACGCGCTGAGGCGCTCCCAGGGCGAAGAGGTGGATTTCGCTCGCGCTGAGCGCCAGCA
  CCAGCTCTACGTGGGCGTGCTGGGCAGCAAGCTGGGGCTGCAGGTGGTGCAGCTGCCGGCCGACGAGAGC
  CTGCCTGACTGCGTGTTCGTGGAGGACGTGGCCGTGGTGTGCGAGGAGACCGCCCTCATCACCCGCCCCG
  GGGCGCCTAGCCGCAGGAAGGAGGTTGACATGATGAAAGAGGCTTTGGAAAAACTTCAGCTCAACATAGT
  AGAGATGAAAGATGAAAATGCAACCTTAGATGGTGGGGACGTCCTATTCACAGGCAGAGAGTTTTTTGTG
  GGCCTTTCCAAAAGGACAAATCAACGAGGTGCTGAGATCTTGGCTGATACTTTCAAGGACTACGCAGTTT
  CCACAGTCCCCGTGGCCGATTCTTTGCATTTAAAGAGTTTCTGCAGCATGGCTGGCCCCAACCTGATCGC
  AATAGGGTCCAGTGAATCTGCGCAGAAGGCCCTCAAGATCATGCAACAGATGAGTGACCACCGTTATGAC
  AAGCTCACTGTACCGGACGACATGGCCGCCAACTGTATATATTTAAATATCCCCAGCAAAGGGCATGTCT
  TGCTGCACCGAACCCCAGAAGAGTACCCAGAAAGCGCAAAGGTTTATGAGAAGCTCAAGGACCATCTACT
  GATCCCTGTGAGCAATTCTGAGATGGAAAAGGTGGACGGCTTGCTCACCTGCTGCTCCGTTTTTATTAAC
  AAGAAGACAGACTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205721 representing NM_022297
  Red=Cloning site Green=Tags(s)
  
  MAGLSHPSVFGRATHAVVRAPPESLCRHALRRSQGEEVDFARAERQHQLYVGVLGSKLGLQVVQLPADES
  LPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFV
  GLSKRTNQRGAEILADTFKDYAVSTVPVADSLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYD
  KLTVPDDMAANCIYLNIPSKGHVLLHRTPEEYPESAKVYEKLKDHLLIPVSNSEMEKVDGLLTCCSVFIN
  KKTDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022297
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022297.1, NM_022297.2, NP_071633.1
• RefSeq Size: 2686
• RefSeq ORF: 858
• Locus ID: 64157
• MW: 31.4 kDa
• Gene Summary: enzyme metabolizes dimethylarginine; involved in regulating the concentration of nitric oxide [RGD, Feb 2006]