Aplnr (NM_031349) Rat Tagged ORF Clone

CAT#: RR205740

Aplnr (Myc-DDK-tagged ORF) - Rat apelin receptor (Aplnr), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aplnr
• Synonyms: Agtrl1; Apj
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205740 representing NM_031349
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATGATGGTTACAACTACTACGGGGCTGACAACCAGTCTGAATGTGACTACGCAGACTGGACGC
  CCTCTGGAGCTCTCATCCCTGCCATCTACATTCTGGTTTTCCTTCTAGGCACCACAGGCAATGGCCTGGT
  GCTCTGGACCGTGTTTTGGAGCAGCCGAGAAAAGAGACGCTCAGCTGACATCTTCATTGCCAGCCTGGCT
  GTGGCTGACTTGACCTTTGTGGTGACTTTGCCACTGTGGGCCACTTATACCTACCGGGAGTTTGACTGGC
  CTTTTGGAACCTTCTCTTGCAAGCTCAGCAGCTACCTCATCTTTGTCAACATGTACGCCAGTGTCTTTTG
  CCTCACCGGCCTCAGCTTTGACCGATACCTGGCCATTGTCAGGCCAGTGGCTAACGCTCGACTAAGGCTG
  CGAGTCAGCGGGGCCGTGGCCACAGCAGTCTTGTGGGTGCTGGCTGCCCTTCTAGCTGTGCCCGTCATGG
  TGTTCCGTTCCACTGACATCCCGGAAAACAGCACCAAGACCCAGTGCTACATGGACTACTCTATGGTGGC
  CACTTCAAACTCAGAGTGGGCCTGGGAGGTGGGCCTTGGGGTGTCCTCCACTGCTGTGGGCTTTGTGGTG
  CCCTTCATCATCATGCTGACCTGTTACTTCTTCATTGCCCAAACCATCGCTGGCCATTTCCGAAAGGAGC
  GCATCGAGGGCCTGCGGAAGAGGCGCCGGCTGCTGAGCATCATCGTGGTGCTGGTGGTGACCTTTGCCCT
  GTGCTGGATGCCTTACCACCTGGTGAAGACTCTCTACATGCTGGGCAATTTGCTGCACTGGCCCTGTGAC
  TTTGACAGCTTCCTCATGAATGTCTTTCCCTATTGCACCTGCATCAGTTACGTCAACAGCTGCCTCAACC
  CCTTCCTCTATGCCTTCTTTGACCCCCGATTTCGCCGAGCCTGCACCTCCATGCTCTGCTGTGACCAGAG
  CGGGTGCAAAGGCAGCCCTCACAGCAGCAGTGCAGAGAAATCTGCCAGTTATTCTTCTGGGCACAGCCAG
  GGCCCCGGCCCCAACATGTGCAAGGGAGGAGAACCGATGCATGAGAAATCTATCCCCTATAGCCAAGAAA
  CCCTTGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205740 representing NM_031349
  Red=Cloning site Green=Tags(s)
  
  MEDDGYNYYGADNQSECDYADWTPSGALIPAIYILVFLLGTTGNGLVLWTVFWSSREKRRSADIFIASLA
  VADLTFVVTLPLWATYTYREFDWPFGTFSCKLSSYLIFVNMYASVFCLTGLSFDRYLAIVRPVANARLRL
  RVSGAVATAVLWVLAALLAVPVMVFRSTDIPENSTKTQCYMDYSMVATSNSEWAWEVGLGVSSTAVGFVV
  PFIIMLTCYFFIAQTIAGHFRKERIEGLRKRRRLLSIIVVLVVTFALCWMPYHLVKTLYMLGNLLHWPCD
  FDSFLMNVFPYCTCISYVNSCLNPFLYAFFDPRFRRACTSMLCCDQSGCKGSPHSSSAEKSASYSSGHSQ
  GPGPNMCKGGEPMHEKSIPYSQETLVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031349
• ORF Size: 1131 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031349.1, NM_031349.2, NP_112639.1
• RefSeq Size: 3670
• RefSeq ORF: 1134
• Locus ID: 83518
• MW: 42.8 kDa
• Gene Summary: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity and plays a role in various processes in adults such as regulation of blood pressure, heart contractility, and heart failure. Also plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone. [UniProtKB/Swiss-Prot Function]