Ak8 (NM_001004266) Rat Tagged ORF Clone

CAT#: RR205759

Ak8 (Myc-DDK-tagged ORF) - Rat HypB, AroK, ADK and rho factor domain containing protein RGD1303144 (RGD1303144), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ak8
• Synonyms: AK 8; RGD1303144
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205759 representing NM_001004266
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTACCTCGGCCCCGCACCGCATCCCCCCAGAAATGCCTCAGTACGGGGAAGACTACCACATCT
  TCGAGATGATGCAGAGCATGCTGGAGCAGCTCCTGATTCACCAGCCAGAGGACCCCATCAGCTTCATGAT
  CAGTCACCTTCGCAGGAACAACGATAATGTGCCGAGAGTTGTGATATTAGGTCCGCCTGCCTCAGGGAAA
  ACCACCATCGCGATGTGGCTCTGCAAACATCTGAACAGCAACCTCATCACCAAGGAGAACTTATTAGAGA
  GAGAGTTTTCCCTCCTGTCTCTGGAAGCCAAGAAACACTACCAGGTTTACAAGAGAGTGCCCAATTCGAC
  TCTTGTCAGCCTGGTCCAGGAGCGTCTGAATGAGGATGACTGCCTCAGAAGGGGCTGGATCCTGGATGGC
  ATCCCCGAGAGGCGGGAGCAGGCTCTGATGATCCAGACCCTGGGACTGGCACCCAAGCACGTCATCGTGC
  TTAGCGCTCCAGACTCCGTCCTGATCGAGAGAAATGTGGGGAAACGGATTGATCCTGTCACTGGAGAGAT
  CTATCACACCACCTTCGACTGGCCCCCTGAGCTTGAAATACAGAACAGGCTGATACAGCCAGAAGGCATC
  TCGGAGATAGACACAGCGAAGAAGCTGCTGGAATACCACAGGCACATCATCAGGATTCTTCCTTCCTACC
  CTAAAATCCTGAAAACTATCAGCTCAGACCAGCCATGTGTGGATGTCTTCTACCAGGCTCTGACCTACGT
  CCAATCTGGCCACCGATGTAATGCCCCGTTCACCCCCAAGGTGCTGCTGTGTGGGCCAATGGGCTGTGGG
  AAGAAACTGCAGGCTGCCCTTCTGTCCCAGAAATATGGCCTCGTGAACATCAGCTGCGGGCAGCTGCTGA
  AGGAAGCTATGGCAGCCGAATCGAGCCTTGGGGACCTCATAGAACCATTCTTTGAAAAGAGGATGACAGT
  TCCAGACAGTATCATCACCAGAGTTCTGACTGAGCGTCTGAAGCAACAAGATTGCATCCAGAAAGGGTGG
  GTGCTGCACGGCTTCCCAAGAGACCTGGACCAGGCCCGGCTGCTGAACTCTATGGGCTACAGTCCCAACA
  GGGTGTTCTTCCTGAATGTGCCGTTGGATTCCATCCTGGAGCGGCTCACACTGAGAAGGACAGACCCTGT
  CACAGGAGAAAGGTTCCATCTCATGTACAAGCCCCCTCCCACCATAGAGGTCCAGGCTCGCCTCCTGCAG
  AACCCCAAGGATTCAGAGGAGTACATCAAGCTCCAAACGGACTTGTTCTATAGGAACTCTGGGGACCTGG
  AGCAGTACTACGATCGGGCCATCATTGTCAACGGTGACCAGGACCCATACACAGTTTTTGAGTATATAGA
  GAGTGGAATCATTAATCCTCTGCCCAGGAAAGTTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205759 representing NM_001004266
  Red=Cloning site Green=Tags(s)
  
  MDATSAPHRIPPEMPQYGEDYHIFEMMQSMLEQLLIHQPEDPISFMISHLRRNNDNVPRVVILGPPASGK
  TTIAMWLCKHLNSNLITKENLLEREFSLLSLEAKKHYQVYKRVPNSTLVSLVQERLNEDDCLRRGWILDG
  IPERREQALMIQTLGLAPKHVIVLSAPDSVLIERNVGKRIDPVTGEIYHTTFDWPPELEIQNRLIQPEGI
  SEIDTAKKLLEYHRHIIRILPSYPKILKTISSDQPCVDVFYQALTYVQSGHRCNAPFTPKVLLCGPMGCG
  KKLQAALLSQKYGLVNISCGQLLKEAMAAESSLGDLIEPFFEKRMTVPDSIITRVLTERLKQQDCIQKGW
  VLHGFPRDLDQARLLNSMGYSPNRVFFLNVPLDSILERLTLRRTDPVTGERFHLMYKPPPTIEVQARLLQ
  NPKDSEEYIKLQTDLFYRNSGDLEQYYDRAIIVNGDQDPYTVFEYIESGIINPLPRKVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004266
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004266.1, NP_001004266.1
• RefSeq Size: 1581 bp
• RefSeq ORF: 1440 bp
• Locus ID: 311833
• Cytogenetics: 3p12
• MW: 55 kDa