Erap1 (NM_030836) Rat Tagged ORF Clone

CAT#: RR205838

Erap1 (Myc-DDK-tagged ORF) - Rat endoplasmic reticulum aminopeptidase 1 (Erap1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Erap1
• Synonyms: Appils; Arts1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205838 representing NM_030836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCTCTTCTTTCCCTAGTATTGACCTTTTTAGCTGTGTCTTCTCCTTCTTGTTGTCAGAACAGTG
  ACACAGCATCTCCAAAAGCTAGTAATGGGGCCTCATTCCCTTGGAATAATATGCGACTTCCTGAGTATAT
  CACCCCAATTCATTATGATCTCATGATCCATGCAAACCTTAGCACTCTGACTTTCTGGGGGAAAACAGAA
  GTAGAAATCACAGTTAGCCAGCCCACCAGCACCATTATCATGCATAGTCACCAGCTGCAAATATCTAAGG
  CCACCCTCAGGAGAGGAGCTGAAGAGATGCTGCCTGAAGAACCACTGAAGCTCATGGAATATTCTGCTCA
  TGAGCAAGTTGCACTGCTGACTGCCCAGCCGCTCCTTGCTGGGTCCGTGTACACAGTTATCATCACCTAT
  GCTGCCAACCTGTCTGAGAATTTCCATGGATTCTATAAAAGCACCTACAGAACACAGGAGGGGGAAAGGA
  GAATACTTGCAGCAACACAGTTTGAACCCACAGCTGCTAGAATGGCTTTTCCCTGCTTTGATGAGCCTGC
  CCTCAAGGCAAGTTTTTCCATCAAGATAAAGAGGGATCCAAGGCACCTGGCCATCTCCAACATGCCCTTG
  GTGAAATCTGTGACTGTTGCTGAAGGACTCATAGAAGACCATTTTGACATCACTGTGAAAATGAGCACCT
  ACCTTGTGGCCTTCATTATTTCTGATTTTAAGTCTGTGAGCAAGATGACTAAGAGTGGAGTCAAGGTCTC
  TGTGTATGCTGTGCCAGACAAGATAAATCAAGCAGATTATGCTCTGGATGCTGCAGTGACTCTTCTAGAG
  TTTTATGAGGATTATTTCAGCATTCCATATCCTTTACCCAAGCAAGATCTTGCTGCAATTCCAGACTTTC
  AGTCTGGTGCTATGGAAAACTGGGGCCTGACCACATACAGAGAATCTGCTTTGTTATATGATAAAGAAAA
  GTCTTCTGCATCCAGTAAGCTTGGCATCACAATGACTGTATCCCATGAACTGGCTCACCAGTGGTTCGGG
  AACTTGGTCACAATGGAATGGTGGAATGACCTTTGGCTCAATGAAGGATTTGCCAAATTTATGGAGTTTG
  TGTCTGTCACTGTGACCCATCCTGAATTAAAAGTTGAAGAATATTTTTTTGGCAAGTGTTTTAATGCAAT
  GGAAGTTGATGCATTAAACTCCTCTCACCCTGTATCCACACCTGTGGAGAATCCTGCACAGATTCGGGAG
  ATGTTTGATGAGGTTTCTTATGAAAAGGGTGCTTGTATTCTGAATATGCTAAGAGATTATCTGAGTGCAG
  ATACATTTAAAAGAGGAATTGTACAGTATCTTCAAAAGTATAGTTATAAAAACACGAAAAATGAGGACCT
  GTGGAATAGCATGATGCATATTTGTCCCACAGATGGCACACAAACAATGGATGGCTTTTGCTCTAGGAAC
  CAACACTCATCATCAACCTCACACTGGCGTCAGGAGGTTATAGATATAAAGAGCATGATGAACACATGGA
  CACTGCAGAAGGGCTTTCCTCTGATAACCATCACTGTAAGAGGGCGGAATGTGCACTTGAAGCAAGAACA
  CTACATGAAAGGTTCTGAATGCTTCCCAGAGACTGGGTCTCTGTGGCATGTTCCGCTGACATTCATTACC
  AGCAAATCAGATTCAGTCCAGAGATTTTTGCTGAAGACAAAGACAGATGTGATCATCCTCCCAGAAGCAG
  TGGAATGGATCAAATTTAATGTAGGAATGAATGGCTATTACATTGTGCATTACGGGGATGATGGATGGGC
  TTCTCTGAATGGCCTCTTAAAAGAAGCACATACAACAATCAGCAGTAATGATCGGGCAAGTCTCATCAAC
  AATGCATTTCAGCTGGTCAGCATCGGAAAGTTATCAATTGAAAAAGCCCTGGATTTAATCCTGTACTTGA
  AAAATGAAACTGAAATTATGCCCATATTCCAAGGTTTGAATGAACTGATACCTATGTATAAGTTAATGGA
  GAAAAGAGATATGGTTGAGGTGGAAACTCAATTCAAGGACTTTCTCCTCAGGCTGCTAAAGGACCTTATT
  AATAAGCAGACCTGGACAGATGAAGGCTCTGTCTCAGAGAGGATGCTCAGGAGCCAGCTCTTACTCCTAG
  CATGTGTGCACAGGTATCAGCTCTGCGTGCAGAGGGCAGAGCGCTATTTCAGAGAGTGGAAGGCATCCAA
  TGGAAACATGAGCCTCCCTATTGATGTGACCTTGGCGGTGTTTGCTGTTGGGGCCCAGAACACAGAAGGC
  TGGGATTTTCTTTATAGTAAATATCAGTCTTCTTTGTCTAGTACCGAGAAAAGCCAAATTGAATTTTCCC
  TCTGCATAAGCCAAGACCCAGAAAAACTTCAGTGGCTTCTAGACCAAAGTTTTAAGGGAGAGATAATAAA
  AACTCAGGAATTCCCACATATTCTCACGCTCATTGGCAGAAACCCAGTGGGTTATCCATTGGCCTGGAAG
  TTTCTGAAGGAAAATTGGAACAAGATTGTACAGAAATTTGAACTTGGCTCATCATCCATAGCTCACATGG
  TAATGGGAACAACAAACCAGTTTTCCACCAGAGCACGTCTTGAAGAGGTAAAGGGGTGTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205838 representing NM_030836
  Red=Cloning site Green=Tags(s)
  
  MPSLLSLVLTFLAVSSPSCCQNSDTASPKASNGASFPWNNMRLPEYITPIHYDLMIHANLSTLTFWGKTE
  VEITVSQPTSTIIMHSHQLQISKATLRRGAEEMLPEEPLKLMEYSAHEQVALLTAQPLLAGSVYTVIITY
  AANLSENFHGFYKSTYRTQEGERRILAATQFEPTAARMAFPCFDEPALKASFSIKIKRDPRHLAISNMPL
  VKSVTVAEGLIEDHFDITVKMSTYLVAFIISDFKSVSKMTKSGVKVSVYAVPDKINQADYALDAAVTLLE
  FYEDYFSIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESALLYDKEKSSASSKLGITMTVSHELAHQWFG
  NLVTMEWWNDLWLNEGFAKFMEFVSVTVTHPELKVEEYFFGKCFNAMEVDALNSSHPVSTPVENPAQIRE
  MFDEVSYEKGACILNMLRDYLSADTFKRGIVQYLQKYSYKNTKNEDLWNSMMHICPTDGTQTMDGFCSRN
  QHSSSTSHWRQEVIDIKSMMNTWTLQKGFPLITITVRGRNVHLKQEHYMKGSECFPETGSLWHVPLTFIT
  SKSDSVQRFLLKTKTDVIILPEAVEWIKFNVGMNGYYIVHYGDDGWASLNGLLKEAHTTISSNDRASLIN
  NAFQLVSIGKLSIEKALDLILYLKNETEIMPIFQGLNELIPMYKLMEKRDMVEVETQFKDFLLRLLKDLI
  NKQTWTDEGSVSERMLRSQLLLLACVHRYQLCVQRAERYFREWKASNGNMSLPIDVTLAVFAVGAQNTEG
  WDFLYSKYQSSLSSTEKSQIEFSLCISQDPEKLQWLLDQSFKGEIIKTQEFPHILTLIGRNPVGYPLAWK
  FLKENWNKIVQKFELGSSSIAHMVMGTTNQFSTRARLEEVKGCM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030836
• ORF Size: 2652 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030836.1, NP_110463.1
• RefSeq Size: 5042
• RefSeq ORF: 2655
• Locus ID: 80897
• MW: 101.2 kDa
• Gene Summary: aminopeptidase enzyme; catalyzes the puromycin-insensitive Zn-dependent hydrolysis of N-terminal Leu; plays a role in processing antigenic precursors for MHC class I presentation [RGD, Feb 2006]