Mcm7 (NM_001004203) Rat Tagged ORF Clone

CAT#: RR205854

Mcm7 (Myc-DDK-tagged ORF) - Rat minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mcm7
• Synonyms: MGC93853
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205854 representing NM_001004203
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTTAAGGACTATGCGATTGAGAAAGAAAAAGTGAAGAAGTTCCTGCAAGAGTTTTATTATGATG
  ATGAGCTTGGAAAGAAGCAGTTCAAGTATGGGACCCAGTTGGTTCATCTGGCTCATCGGGAACAAGTGGC
  ATTGTACGTGGACCTAGATGATGTAGCTGAAGATGACCCTGAGTTGGTCGACTCAATTTGCGAGAATGCC
  AAGCGCTACTCGAGGCTGTTTGCTGACGTAGTACAGGAGCTTCTGCCTGAGTACAAGGAGAAGGAGGTGG
  TGAATAAGGATGTCCTGGATGTTTACATTGAACACCGATTGATGATGGAGCAGCGGAGCAGAGACCCTGG
  GGCAGTCCGGAACCCTCAAAACCAGTATCCTTCTGAGCTCATGCGTAGATTTGAGTTGTATTTCCAAGGC
  CCAAGTAGCAGCAAGCCTCGAGTGATCCGGGAAGTACGGGCTGATTCTGTAGGGAAATTGTTAACTGTGC
  GTGGGATTGTCACTCGTGTGTCTGAAGTCAAGCCCAGGATGGTGGTGGCCACATACACTTGCGATCAATG
  TGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACCTTCATGCCTCTGATCATGTGCCCCAGCCAGGAG
  TGCCAAACCAACCGCTCAGGGGGGCGGCTGTACCTACAGACTCGTGGCTCCAAATTTATTAAATTCCAGG
  AAATGAAGATTCAAGAACATAGTGACCAAGTTCCGGTGGGAAACATCCCTCGGAGCATCACAGTGGTTCT
  AGAAGGAGAGAACACAAGGATTGCCCAGCCTGGTGACCATGTCAGCGTCACTGGCATCTTCTTGCCAGTC
  TTGCGCACAGGGTTCCAACAGATGGCACAGGGCTTACTCTCGGAAACTTACCTGGAAGCCCATCGGGTTG
  TGAAGATGACGAAGAGCGAGGATGATGTGTCTGGGGCCGGAGAGCTGAGTGCCGAGGAGCTGAAGCAGAT
  TGCCGAGGAAGATTTCTATGAAAAGCTGGCTGCTTCTATTGCTCCGGAGATCTATGGTCATGAAGATGTG
  AAGAAGGCGCTCTTACTGCTCCTGGTGGGTGGTGTGGACCAGTCTCCTCAAGGCATGAAGATAAGAGGAA
  ACATCCACATCTGCCTTATGGGAGATCCTGGTGTGGCCAAGTCTCAGCTCCTGTCTTACATTGACCGTCT
  GGCGCCCCGAAGTCAGTACACAACGGGCCGGGGCTCCTCTGGTGTAGGGCTCACAGCAGCTGTGTTGAGA
  GACTCTGTGAGTGGGGAATTGACCTTAGAGGGTGGTGCCCTGGTGCTGGCTGACCAGGGCGTGTGCTGCA
  TTGATGAGTTTGACAAGATGGCAGAAGCTGACCGCACAGCCATCCATGAGGTTATGGAGCAGCAGACCAT
  CTCCATTGCCAAGGCAGGAATTCTCACCACACTCAATGCCCGATGCTCTATCCTGGCTGCTGCCAACCCT
  GCCTATGGACGGTATAACCCCCGCCGCAGTTTGGAGCAGAACATACAGCTTCCTGCTGCTCTGCTCTCCC
  GATTCGACCTCCTCTGGCTGATTCAGGACCGGCCCGACAGAGACAACGACCTCCGGTTGGCCCAGCACAT
  AACCTATGTCCACCAGCACAGCCGGCAGCCCCCTGCTCAATTTGAACCTTTGGACATGAAACTTATGAGA
  CGGTACATTGCCATGTGCCGCGAGAGGCAGCCCACAGTGCCTGACTCTCTGGCTGACTACATCACTGCAG
  CGTATGTGGAGATGAGGCGAGAGGCCCGGGCCAGTAAGGATGCCACCTACACTTCTGCCCGGACCCTGCT
  GGCCATTCTCCGACTTTCTACTGCTCTGGCTCGCCTTCGGATGGTAGATATTGTGGAGAAGGAAGATGTG
  AATGAAGCCATAAGGTTGATGGAGATGTCAAAGGACTCCCTTCTGGGTGAAAAGGGACAAACGGCTAGGA
  CCCAGAGGCCAGCAGATGTGATATTTGCTACCGTCCGTGAGTTGGTATCAGGGGGACGGAGTGTCCGCTT
  TTCTGAGGCTGAACAGCGCTGCATATCACGTGGCTTCACACCAGCCCAGTTCCAGGCAGCTCTGGATGAA
  TATGAGGAGCTAAATGTCTGGCAGGTCAATACTTCTCGGACACGGATCACCTTTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205854 representing NM_001004203
  Red=Cloning site Green=Tags(s)
  
  MALKDYAIEKEKVKKFLQEFYYDDELGKKQFKYGTQLVHLAHREQVALYVDLDDVAEDDPELVDSICENA
  KRYSRLFADVVQELLPEYKEKEVVNKDVLDVYIEHRLMMEQRSRDPGAVRNPQNQYPSELMRRFELYFQG
  PSSSKPRVIREVRADSVGKLLTVRGIVTRVSEVKPRMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQE
  CQTNRSGGRLYLQTRGSKFIKFQEMKIQEHSDQVPVGNIPRSITVVLEGENTRIAQPGDHVSVTGIFLPV
  LRTGFQQMAQGLLSETYLEAHRVVKMTKSEDDVSGAGELSAEELKQIAEEDFYEKLAASIAPEIYGHEDV
  KKALLLLLVGGVDQSPQGMKIRGNIHICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
  DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANP
  AYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPAQFEPLDMKLMR
  RYIAMCRERQPTVPDSLADYITAAYVEMRREARASKDATYTSARTLLAILRLSTALARLRMVDIVEKEDV
  NEAIRLMEMSKDSLLGEKGQTARTQRPADVIFATVRELVSGGRSVRFSEAEQRCISRGFTPAQFQAALDE
  YEELNVWQVNTSRTRITFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004203
• ORF Size: 2157 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004203.1, NM_001004203.2, NM_001004203.3, NP_001004203.3
• RefSeq Size: 2357 bp
• RefSeq ORF: 2160 bp
• Locus ID: 288532
• Cytogenetics: 12q11
• MW: 81.1 kDa
• Gene Summary: member of a family of mini-chromosome maintenance (MCM) proteins; required for the initiation and elongation stages of DNA replication; key component of the pre-replication complex (pre_RC); often used as a marker for cell proliferation [RGD, Feb 2006]