Slc11a1 (NM_001031658) Rat Tagged ORF Clone

CAT#: RR205888

Slc11a1 (Myc-DDK-tagged ORF) - Rat solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (Slc11a1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc11a1
• Synonyms: Bcg; Itg; Lsh; Nramp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205888 representing NM_001031658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCGTGACAAAAACCCCCAGAGGGTGAACAGGCCCAGTTATGGCTCCATTTCCAGCCTGCCTAGCC
  CAGCACCTCAGCCAGAGCCTTCCAGGAACACCTACCTGAGTGAGAAGATTCCCATCCCCAGCACGGAACA
  GCTGCTCTGGGTGCTGCTCTGGGCCACTGTGCTAGGCTTGCTCTGCCAGCGGCTGGCTGCCCGGCTGGGC
  GTGGTGACAGGCAAGGATCTGGGTGAAATCTGCCATCTCTACTACCCTAAGGTGCCCCGCATCCTCCTCT
  GGCTGACCATTGAGCTGGCCATTGTGGGCTCAGACATGCAGGAAGTCATCGGAACGGCTATCTCCTTCAA
  TCTGCTCTCCGCTGGACGCATCCCCCTGTGGGGTGGCGTACTGATCACTATTGTGGATACCTTCTTCTTC
  CTCTTCCTGGATAACTATGGTTTGCGCAAGTTGGAAGCGTTTTTTGGGTTCCTCGTTACCATAATGGCTT
  TGACCTTCGGTTATGAGTATGTGGTGGCTCGTCCATCTCAGGGAGCCCTTCTTAAGGGCCTGTTCCTGCC
  CTCCTGCCCGGGCTGTGGGCAGCCTGAGCTGCTGCAGGCAGTGGGCATCGTTGGTGCCATCATCATGCCT
  CATAACATCTACCTGCACTCGGCCTTGGTCAAGTCTAGAGAGGTAGACAGAACTCGCCGGGGGGATGTTC
  GAGAAGCCAACATGTACTTCCTGACTGAGGCCACCATCGCCCTCTTCGTGTCATTCATCATCAACCTCTT
  CGTCATGGCTGTTTTTGGTCAGGCCTTCTACCAGCAAACCAATGAGGAAGCGTTCAACATCTGTGCCAAC
  AGCAGCCTCCACAACTATGCTAAGATCTTCCCCAGGGACAATAACACAGTGTCAGTGGATATTTATCAAG
  GAGGTGTGATCCTAGGCTGTCTCTTTGGCCCTGCGGCCCTCTACATCTGGGCAGTAGGTCTCCTGGCAGC
  GGGTCAGAGTTCTACTATGACCGGCACCTATGCAGGACAATTCGTGATGGAGGGTTTCCTTAAGCTGCGA
  TGGTCTCGATTTGCACGCGTCCTTCTCACTCGCTCTTGCGCTATCCTGCCCACTGTGCTGGTGGCTGTCT
  TCCGAGACCTGAGGGACCTGTCCGGCCTCAACGATCTACTCAATGTTCTGCAGAGTCTACTGCTGCCCTT
  TGCTGTGCTGCCCATTCTGACTTTCACCAGCATGCCAGCCGTCATGCAGGAGTTTGCCAATGGCTGGTTG
  AGCAAAGTCATTACTTCCTGCATCATGGCACTGGTCTGCGCCATCAACCTGTACTTCGTGATCAGCTACC
  TGCCCAGCCTCCCGCACCCTGCCTACTTTGGCCTTGTGGCTCTGCTCGCCATAGGTTACTTGGGCCTTAC
  TGCTTATCTGGCCTGGACCTGTTGCATTGCCCATGGAGCCAAGTTTCTGACCCACAGCTCCCACCAGCGC
  TTCCTGTATGGACTCCCTATCGAGGAGCAGGAAGGCAGGGAGGGTTCCGGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205888 representing NM_001031658
  Red=Cloning site Green=Tags(s)
  
  MIRDKNPQRVNRPSYGSISSLPSPAPQPEPSRNTYLSEKIPIPSTEQLLWVLLWATVLGLLCQRLAARLG
  VVTGKDLGEICHLYYPKVPRILLWLTIELAIVGSDMQEVIGTAISFNLLSAGRIPLWGGVLITIVDTFFF
  LFLDNYGLRKLEAFFGFLVTIMALTFGYEYVVARPSQGALLKGLFLPSCPGCGQPELLQAVGIVGAIIMP
  HNIYLHSALVKSREVDRTRRGDVREANMYFLTEATIALFVSFIINLFVMAVFGQAFYQQTNEEAFNICAN
  SSLHNYAKIFPRDNNTVSVDIYQGGVILGCLFGPAALYIWAVGLLAAGQSSTMTGTYAGQFVMEGFLKLR
  WSRFARVLLTRSCAILPTVLVAVFRDLRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPAVMQEFANGWL
  SKVITSCIMALVCAINLYFVISYLPSLPHPAYFGLVALLAIGYLGLTAYLAWTCCIAHGAKFLTHSSHQR
  FLYGLPIEEQEGREGSG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031658
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031658.1, NP_001026828.1
• RefSeq Size: 2012 bp
• RefSeq ORF: 1524 bp
• Locus ID: 316519
• Cytogenetics: 9q33
• MW: 55.9 kDa
• Gene Summary: putative metal ion transporter; mouse homolog is a macrophage protein associated with resistance or susceptibility to intracellular pathogens [RGD, Feb 2006]