Clec14a (NM_001014077) Rat Tagged ORF Clone

CAT#: RR205904

Clec14a (Myc-DDK-tagged ORF) - Rat C-type lectin domain family 14, member a (Clec14a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clec14a
• Synonyms: RGD1306232
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205904 representing NM_001014077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCGGCTCTTGCCCTGTGCCTCCTCTGTCCTGCGTTCTGGCCCCGGCCAGGGAATGGCGAGCACC
  CCACGGCCGATCGCGCAGTTTGTTCGGTCCCGGGGGCTTGCTACAGCCTGCACCACGCAGCCATGAAGAG
  GAGGGCGGCGGAGGAGGCCTGTAGCTTGCGCGGCGGGACTCTCAGCACCGTGCAGTCCGGCTCTGAGTTG
  CAAGCTGTGCTCAAGCTCTTTCGTGCAGGTCCCGGGCCTGGTGGAGGCTCCAAAGACCTAATGTTCTGGG
  TGGCTCTGGAGCGTGACCGTTCACAGTGCACCCAGGAGAGAGAGCCTTTGAGGGGTTTCTCCTGGTTGTA
  CACGGACTCAGAAGACTCGGAGAACAGAACACTACCTTGGGTGGAAGAGCCACAACTTTCTTGCACTGTA
  CGAAAGTGCGCTGTGCTCCAGGCCACCGGGGGAGTGGAGCCTGCTGGCTGGAAGGAGATGCGCTGTCAAC
  AGCGCGCCGATGGCTACCTGTGCAAGTACCAGTTTGAGGCTCTGTGCCCTGCGCCTCGCCCGGGAGCCGC
  CTCTAACTTAAGTTTCCAAGCTCCCTTCCGGCTGAGCAGTTCTGCCCTGGACTTCAGCCCTCCTGGGACC
  GAGGTGAGTGCGATGTGTCCCAGGGACTTCTCAGTCTCCTCCATCTGCGTCCAGGAAGAGACAGGCGCAC
  GCTGGGATGGGCTTTTCCCTGGGAGCTTGCTCTGCCCCTGTTCTGGGAGGTACCTCCTTGCTGGCAAGTG
  CGTGGAGCTCGCTGACTGCCTAGACTACTTGGGAAACTTCGCCTGCGAATGTGCTGTGGGTTTTGAGCTA
  GGGAAGGACAAACGCTCTTGTGAGACCAAAGCGGAAGGCCAACTAACCCTCGAGGGGACCAAGTTGCCCA
  CTAGAAATGTATCAGCCACTCCAGCCAGTCCTGTGACAAACAAATCGTGGCCAGGTCAGGTCTATGACAA
  GCCAGGAGAGATGCCCCAGGTCACTGGACAAGACCGTATTGCGACATCTGTTCCTGAGATTCTTCAGTGG
  GGAACACAGAGTACTTTACCTACTGTTCAAACGTCCCCACAAACCAAGCCAAAAGTCACCATCACACCAT
  CAGGAAGTATGATGCCCACATTGAACTTCGCATCTTCCCCTCCTGTTTCTCTGACTTTCGACTCCTCCTC
  CACGGTGGTCTTCGTACTTGTGAGCATAGCAGTAATAGTTTTGGTACTCTTGACCATTACAGTGCTGGGA
  CTTTTCAAACTCTGCTTCCACAAAAGCCCTTCCTCCAGGACAAGGAAGGGAGCTTTGGACTCACAGGGTG
  TGGAATGTGATGCAGAGGCCACCTCTCTGCGGCACAATTCTGCACAGTGCACGGACACTGGAGGGAAGGG
  TGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205904 representing NM_001014077
  Red=Cloning site Green=Tags(s)
  
  MRPALALCLLCPAFWPRPGNGEHPTADRAVCSVPGACYSLHHAAMKRRAAEEACSLRGGTLSTVQSGSEL
  QAVLKLFRAGPGPGGGSKDLMFWVALERDRSQCTQEREPLRGFSWLYTDSEDSENRTLPWVEEPQLSCTV
  RKCAVLQATGGVEPAGWKEMRCQQRADGYLCKYQFEALCPAPRPGAASNLSFQAPFRLSSSALDFSPPGT
  EVSAMCPRDFSVSSICVQEETGARWDGLFPGSLLCPCSGRYLLAGKCVELADCLDYLGNFACECAVGFEL
  GKDKRSCETKAEGQLTLEGTKLPTRNVSATPASPVTNKSWPGQVYDKPGEMPQVTGQDRIATSVPEILQW
  GTQSTLPTVQTSPQTKPKVTITPSGSMMPTLNFASSPPVSLTFDSSSTVVFVLVSIAVIVLVLLTITVLG
  LFKLCFHKSPSSRTRKGALDSQGVECDAEATSLRHNSAQCTDTGGKGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014077
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014077.1, NP_001014099.1
• RefSeq Size: 3165 bp
• RefSeq ORF: 1407 bp
• Locus ID: 314148
• Cytogenetics: 6q23
• MW: 50.2 kDa